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Lasers in Medical Science

, Volume 35, Issue 2, pp 365–372 | Cite as

Prolonged post-stimulation response induced by 980-nm infrared neural stimulation in the rat primary motor cortex

  • Manqing Wang
  • Qingling Xia
  • Fei Peng
  • Bin Jiang
  • Lin Chen
  • Xiaoying Wu
  • Xiaolin Zheng
  • Xing Wang
  • Tian TianEmail author
  • Wensheng HouEmail author
Original Article
  • 130 Downloads

Abstract

The post-stimulation response of neural activities plays an important role to evaluate the effectiveness and safety of neural modulation techniques. Previous studies have established the capability of infrared neural modulation (INM) on neural firing regulation in the central nervous system (CNS); however, the dynamic neural activity after the laser offset has not been well characterized yet. We applied 980-nm infrared diode laser light to irradiate the primary motor cortex of rats, and tungsten electrode was inserted to record the single-unit activity of neurons at the depth of 800–1000 μm (layer V of primary motor cortex). The neural activities were assessed through the change of neural firing rate and firing pattern pre- and post-stimulation with various radiant exposures. The results showed that the 980-nm laser could modulate the firing properties of neurons in the deep layer of the cortex. More neurons with post-stimulation response (78% vs. 83%) were observed at higher stimulation intensity (0.803 J/cm2 vs. 1.071 J/cm2, respectively). The change of firing rate also increased with radiant exposures increasing, and the response lasted up to 4.5 s at 1.071 J/cm2, which was significantly longer than the theoretical thermal relaxation time. Moreover, the increasing Fano factors indicated the irregularity firing pattern of post-stimulation response. Our results confirmed that neural activity maintained a prolonged post-stimulation response after INM, which may provide necessary measurable data for optimization of INM applications in CNS.

Keywords

Infrared neural stimulation Short-wavelength infrared Primary motor cortex Post-stimulation response 

Notes

Funding information

This work was supported by grants from the National Natural Science Foundation of China (No. 31771069, 31872751, 31271060), the Fundamental Research Funds for the Central Universities (No.106112016CDJZR238807), and the China Scholarship Council.

Compliance with ethical standards

Ethical approval

All procedures performed in the studies involving animals were in accordance with the ethical standards of the institution at which the protocol was approved.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Manqing Wang
    • 1
  • Qingling Xia
    • 1
  • Fei Peng
    • 1
  • Bin Jiang
    • 1
  • Lin Chen
    • 1
    • 2
  • Xiaoying Wu
    • 1
    • 3
  • Xiaolin Zheng
    • 1
    • 2
  • Xing Wang
    • 1
    • 2
    • 3
  • Tian Tian
    • 1
    Email author
  • Wensheng Hou
    • 1
    • 2
    • 3
    Email author
  1. 1.Key Laboratory of Biorheological Science and Technology of Ministry of EducationChongqing UniversityChongqingChina
  2. 2.Chongqing Medical Electronics Engineering Technology Research CenterChongqing UniversityChongqing 400044China
  3. 3.Chongqing Collaborative Innovation Center for Brain ScienceChongqing UniversityChongqing 400044China

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