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Intravenous laser wavelength radiation effect on LCAT, PON1, catalase, and FRAP in diabetic rats

  • Ahmad AmjadiEmail author
  • Hossein Mirmiranpour
  • Seyed Omid Sobhani
  • Niloofar Moazami Goudarzi
Original Article
  • 25 Downloads

Abstract

The main purpose of this study is to evaluate the effect of intravenous irradiation of different low-level laser wavelengths on the activity of lecithin-cholesterol acyltransferase (LCAT), paraoxonase (PON1), catalase, and ferric reducing ability of plasma (FRAP) in diabetic rats. First, diabetes was induced in rats using streptozotocin (STZ). Enzymes’ activity was measured in the blood samples and compared before and after intravenous laser blood irradiation. We used four continuous-wave lasers—IR (λ = 808 nm), Red (λ = 638 nm), Green (λ = 532 nm), and Blue (λ = 450 nm)—to compare the wavelength’s effect on different enzymes’ activity. Laser power was fixed at 0.01 mW and laser energy was changed by 2-, 4-, 6-, and 8-min time of radiations.

The enzymes’ activity of blood samples was measured 2, 6, and 24 h after radiation. The results show an increase in the activity of different enzymes when compare with diabetic non-radiated samples. More importantly, with a constant laser energy, the enzymes’ activity increased with decreasing laser wavelength. It is important to note that with a constant laser energy, as the wavelength decreases, the photon energy increases and the number of photons decrease, while the enzyme’s activity elevation increases. As a result, we can conclude that in intravenous low-level laser therapy, photon energy is more important than the number of photons even if their product, energy, is kept constant.

Keywords

LCAT Catalase PON1 FRAP Activity Low-level laser therapy Laser wavelength 

Notes

Acknowledgments

Lasers were supplied by International Faran Tech Co. We thank Miss Salile Khandani and Miss Nafise Goli for their help. Special thanks to Dr. Marjaneh Hejazi for fruitful discussion.

Funding information

This project was supported by Sharif Applied Physics research centre at Sharif University of Technology.

Compliance with ethical standards

Ethical considerations

The study protocol was approved by the animal ethics review committee, in accordance with the guidelines for the care and use of laboratory animals prepared by Tehran University. Informed consent of this investigation has been ordered by Medical Physics and Laser Lab of Physic Department at Sharif University of Technology.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Ahmad Amjadi
    • 1
    Email author
  • Hossein Mirmiranpour
    • 2
  • Seyed Omid Sobhani
    • 1
  • Niloofar Moazami Goudarzi
    • 1
    • 3
  1. 1.Laser and Medical Physics Lab, Department of PhysicsSharif University of TechnologyTehranIran
  2. 2.Endocrinology and Metabolism Research Center (EMRC), Valiasr Hospital, School of MedicineTehran University of Medical ScienceTehranIran
  3. 3.Department of Physics and AstronomyGhent UniversityGhentBelgium

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