Lasers in Medical Science

, Volume 34, Issue 9, pp 1781–1790 | Cite as

Topical and intradermal delivery of PpIX precursors for photodynamic therapy with intense pulsed light on porcine skin model

  • Michelle Barreto RequenaEmail author
  • José Dirceu Vollet Filho
  • Andrigo Barboza de Nardi
  • Andre Escobar
  • Rozana da Rocha Adams
  • Vanderlei Salvador Bagnato
  • Priscila Fernanda Campos de Menezes
Original Article


In order to purposely decrease the time of the photodynamic therapy (PDT) sessions, this study evaluated the effects of PDT using topical and intradermal delivery of two protoporphyrin (PpIX) precursors with intense pulsed light (IPL) as irradiation source. This study was performed on porcine skin model, using an IPL commercial device (Intense Pulse Light, HKS801). IPL effect on different administration methods of two PpIX precursors (ALA and MAL) was investigated: a topical cream application and an intradermal application using a needle-free, high-pressure injection system. Fluorescence investigation showed that PpIX distribution by needle-free injection was more homogeneous than that by cream, suggesting that a shorter drug-light interval in PDT protocols is possible. The damage induced by IPL-PDT assessed by histological analysis mostly shows modifications in collagens fibers and inflammation signals, both expected for PDT. This study suggested an alternative protocol for the PDT treatment, possibility half of the incubation time and with just 3 min of irradiation, making the IPL-PDT, even more, promising for the clinical treatment.


Photodynamic therapy Intense pulsed light Porcine skin Aminolevulinic acid Methyl aminolevulinate 



We acknowledge the support from the Center for Research in Optics and Photonics (CEPOF - FAPESP CePID program grant 2013/07276-1).


This study was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and by grants 2013/07276-1 (CePOF), 2009/54035-4 (EMU) of São Paulo Research Foundation (FAPESP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Michelle Barreto Requena
    • 1
    Email author
  • José Dirceu Vollet Filho
    • 1
    • 2
  • Andrigo Barboza de Nardi
    • 3
  • Andre Escobar
    • 3
  • Rozana da Rocha Adams
    • 3
  • Vanderlei Salvador Bagnato
    • 1
  • Priscila Fernanda Campos de Menezes
    • 1
  1. 1.São Carlos Institute of PhysicsUniversity of São Paulo (USP)São CarlosBrazil
  2. 2.Institute of Geosciences and Exact SciencesSão Paulo State University (UNESP)Rio ClaroBrazil
  3. 3.College of Agricultural and Veterinary SciencesSão Paulo State University (UNESP)JaboticabalBrazil

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