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Lasers in Medical Science

, Volume 34, Issue 7, pp 1457–1464 | Cite as

Effectiveness of 405-nm blue LED light for degradation of Candida biofilms formed on PMMA denture base resin

  • Chiaki Tsutsumi-AraiEmail author
  • Yuki Arai
  • Chika Terada-Ito
  • Yusuke Takebe
  • Shinji Ide
  • Hirochika Umeki
  • Seiko Tatehara
  • Reiko Tokuyama-Toda
  • Noriyuki Wakabayashi
  • Kazuhito Satomura
Original Article
  • 163 Downloads

Abstract

This study investigated (i) the degradation effect of 405-nm blue light-emitting diode (LED) light irradiation on Candida albicans and C. glabrata biofilms formed on denture base resin and (ii) the effects of 405-nm blue LED light irradiation on the mechanical and surface characteristics of the resin. Polymethyl methacrylate denture base resin discs were prepared, and C. albicans or C. glabrata biofilms formed on the denture base resin discs. Each biofilm was irradiated with 405-nm blue LED light under a constant output power (280 mW/cm2) for different times in a moisture chamber with 100% relative humidity. Postirradiation, each biofilm was analyzed using a colony-forming unit assay, fluorescence microscopy, and scanning electron microscopy (SEM). Parallelepiped specimens of acrylic resin were prepared, and changes in their flexural strength (FS), flexural modulus (FM), and surface roughness (Ra) preirradiation and postirradiation with 405-nm blue LED light were evaluated. Irradiation for 30 min completely inhibited colony formation in both Candida species. Fluorescence microscopy showed that almost all Candida cells were killed because of irradiation. SEM images showed various cell damage patterns, such as wrinkles, shrinkage, and cell surface damage. An increase in FS was noted postirradiation, but no significant changes were observed in FM and Ra preirradiation and postirradiation. In conclusion, irradiation with 405-nm blue LED light induces degradation of C. albicans and C. glabrata biofilms on denture base resin, even in the absence of photosensitizers, without resin surface deterioration.

Keywords

405-nm LED Candida Biofilm Denture stomatitis Fungistatic 

Notes

Acknowledgments

The authors wish to thank the Osada Electric Co. Ltd. for its technical support concerning an irradiation device equipped with a 405-nm blue light-emitting diode (LED).

Funding

This work was supported by JSPS KAKENHI Grant Number 17K17156, 18K17136, and 17H04411.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Chiaki Tsutsumi-Arai
    • 1
    Email author
  • Yuki Arai
    • 2
  • Chika Terada-Ito
    • 1
  • Yusuke Takebe
    • 1
  • Shinji Ide
    • 1
  • Hirochika Umeki
    • 1
  • Seiko Tatehara
    • 1
  • Reiko Tokuyama-Toda
    • 1
  • Noriyuki Wakabayashi
    • 2
  • Kazuhito Satomura
    • 1
  1. 1.Department of Oral Medicine and StomatologyTsurumi University School of Dental MedicineYokohamaJapan
  2. 2.Department of Removable Partial Prosthodontics, Graduate SchoolTokyo Medical and Dental University (TMDU)TokyoJapan

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