Lasers in Medical Science

, Volume 34, Issue 6, pp 1261–1263 | Cite as

Methodological correctness of a recent publication on pain entitled “Evaluating the effect of photobiomodulation with a 940-nm diode laser on post-operative pain in periodontal flap surgery”

  • Vahid RakhshanEmail author
Letter to the Editor

Dear editor,

I read with great interest the excellent study of Heidari et al. [1], which was concerned with the efficacy of photobiomodulation in alleviating pain after periodontal surgery. It was a nice split-mouth randomized clinical trial with numerous advantages.

However, I had some questions and suggestions.
  1. 1.

    I would appreciate if the authors can explain how this particular sample size had been determined.

  2. 2.

    The current design looked like a repeated-measures design. So, it would be appropriate to use a repeated-measures ANOVA (or a similar test) instead of paired t tests. If it is not possible to conduct an RM-ANOVA now (for example, lack of raw data), at least, the multiple comparison problems should be addressed using methods such as the Bonferroni correction, i.e., by reducing the level of significance from 0.05 to 0.007, and considering any P values above 0.007 as non-significant.

  3. 3.

    If the level of significance is to be adjusted to 0.007, it would be needed for the P...


Source of funding

The study was self-funded by the author.

Compliance with ethical standards

Conflict of interest

The author declares that he has no conflict of interest.


  1. 1.
    Heidari M, Fekrazad R, Sobouti F, Moharrami M, Azizi S, Nokhbatolfoghahaei H, Khatami M (2018) Evaluating the effect of photobiomodulation with a 940-nm diode laser on post-operative pain in periodontal flap surgery. Lasers Med Sci 33:1639–1645CrossRefGoogle Scholar
  2. 2.
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    Cho H, David MC, Lynham AJ, Hsu E (2018) Effectiveness of irrigation with chlorhexidine after removal of mandibular third molars: a randomised controlled trial. Br J Oral Maxillofac Surg 56(1):54–59. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Cognitive NeuroscienceInstitute for Cognitive Science StudiesTehranIran
  2. 2.Department of Dental Anatomy, Dental FacultyAzad UniversityTehranIran

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