Lasers in Medical Science

, Volume 34, Issue 7, pp 1333–1343 | Cite as

In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts

  • Daíse Raquel Maldaner
  • Verônica Farina Azzolin
  • Fernanda BarbisanEmail author
  • Moisés Henrique Mastela
  • Cibele Ferreira Teixeira
  • Alexandre Dihel
  • Thiago Duarte
  • Neida Luiza Pellenz
  • Luiz Fernando Cuozzo Lemos
  • Carla Maria Uggeri Negretto
  • Ivana Beatrice Mânica da Cruz
  • Marta Maria Medeiros Frescura Duarte
Original Article


Skin aging is a complex biological process induced by intrinsic and extrinsic factors which is characterized by clinical and cellular changes, especially dermal fibroblasts. It is possible that, some procedures, such as low-level laser therapy (LLLT), could decelerate this process. To test this hypothesis, this study evaluated the in vitro LLLT on dermal fibroblast cell line (HFF-1) with premature senescence H2O2-induced. HFF-1 cells were cultured in standardized conditions, and initially H2O2 exposed at different concentrations. Fibroblasts were also just exposed at different LLLT (660 nm) doses. From these curves, the lowest H2O2 concentration that induced indicators of premature senescence and the lowest LLLT doses that triggered fibroblast proliferation were used in all assays. Cellular mortality, proliferation, and the levels of oxidative, inflammatory cytokines, apoptotic markers, and of two growth signaling molecules (FGF-1 and KGF) were compared among treatments. The H2O2 at 50 μM concentration induced some fibroblast senescence markers and for LLLT, the best dose for treatment was 4 J (p < 0.001). The interaction between H2O2 at 50 μM and LLLT at 4 J showed partially reversion of the higher levels of DNA oxidation, CASP 3, CASP 8, IL-1B, IL-6, and INFy induced by H2O2 exposure. LLLT also trigger increase of IL-10 anti-inflammatory cytokine, FGF-1 and KGF levels. Cellular proliferation was also improved when fibroblasts treated with H2O2 were exposed to LLLT (p < 0.001). These results suggest that in fibroblast with some senescence characteristics H2O2-induced, the LLLT presented an important protective and proliferative action, reverting partially or totally negative effects triggering by H2O2.


Skin Aging Fibroblast Low-level laser 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

Since the study used commercial cell lines, it is not necessary to submit to an ethics committee.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Daíse Raquel Maldaner
    • 1
  • Verônica Farina Azzolin
    • 2
  • Fernanda Barbisan
    • 2
    Email author
  • Moisés Henrique Mastela
    • 2
  • Cibele Ferreira Teixeira
    • 1
  • Alexandre Dihel
    • 3
  • Thiago Duarte
    • 1
  • Neida Luiza Pellenz
    • 1
  • Luiz Fernando Cuozzo Lemos
    • 2
  • Carla Maria Uggeri Negretto
    • 4
  • Ivana Beatrice Mânica da Cruz
    • 1
    • 2
  • Marta Maria Medeiros Frescura Duarte
    • 1
    • 4
  1. 1.Postgraduate Program of PharmacologyFederal University of Santa Maria (UFSM)Santa MariaBrazil
  2. 2.Postgraduate Program of GerontologyFederal University of Santa Maria (UFSM)Santa MariaBrazil
  3. 3.Biogenomic LaboratoryFederal University of Santa Maria (UFSM)Santa MariaBrazil
  4. 4.Lutheran University of BrazilSanta MariaBrazil

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