Raman spectroscopy applications in rheumatology

  • Corina D. Hosu
  • Vlad Moisoiu
  • Andrei Stefancu
  • Elisabeta Antonescu
  • Loredana F. Leopold
  • Nicolae LeopoldEmail author
  • Daniela Fodor
Review Article


Raman spectroscopy is a type of vibrational spectroscopy based on the inelastic scattering of photons, which has attracted much attention due to its potential clinical application in rheumatology. In this review, we discuss the typical spectral features of cartilage, bone, synovial fluid, and pathologic crystal deposits, as well as methods of amplifying the Raman signal of biofluids such as drop-coating deposition Raman spectroscopy. Further, applications of Raman and drop-coating deposition Raman spectroscopy in osteoarthritis are described, highlighting the clinical potential of these methods. We also discuss the role of Raman and related techniques in analyzing pathologic crystals such as monosodium urate, calcium pyrophosphate dihydrate, and hydroxyapatite. The results presented in this review demonstrate that Raman spectroscopy has grown past the stage of proof-of-concept, especially in the case of pathologies involving crystal depositions such as gout and calcium pyrophosphate deposition disease , for which the method has been validated on large number of samples. As the medical community becomes more and more aware of Raman spectroscopy, it is envisioned that it will become a standard technique in the near future.


Rheumatology Raman spectroscopy Osteoarthritis Gout Calcium pyrophosphate deposition disease Ectopic calcifications 



This work was supported by a grant from the Romanian Ministry of Research and Innovation, CCCDI-UEFISCDI, project number PN-III-P1–1.2-PCCDI-2017-0056, within PNCDI III. A.S. also acknowledges support from the College for Advanced Performance Studies, Babeș-Bolyai University, Cluj-Napoca, Romania.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Corina D. Hosu
    • 1
  • Vlad Moisoiu
    • 2
  • Andrei Stefancu
    • 2
  • Elisabeta Antonescu
    • 3
  • Loredana F. Leopold
    • 4
  • Nicolae Leopold
    • 2
    Email author
  • Daniela Fodor
    • 1
  1. 1.2nd Department of Internal Medicine, Iuliu Hatieganu University of Medicine and PharmacyCluj-NapocaRomania
  2. 2.Faculty of PhysicsBabeș-Bolyai UniversityCluj-NapocaRomania
  3. 3.Faculty of MedicineLucian Blaga UniversitySibiuRomania
  4. 4.Faculty of Food Science and TechnologyUniversity of Agricultural Sciences and Veterinary MedicineCluj-NapocaRomania

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