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Lasers in Medical Science

, Volume 34, Issue 3, pp 537–546 | Cite as

Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation

  • Luana C. Pires
  • Fernando P. S. Guastaldi
  • Andressa V. B. Nogueira
  • Nilson T. C. Oliveira
  • Antonio C. Guastaldi
  • Joni A. CirelliEmail author
Original Article
  • 73 Downloads

Abstract

Perform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy (FEG-SEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD). The wettability of the samples was evaluated by contact angle measurement. Murine preosteoblastic cells MC3T3-E1 were cultured onto the experimental disks for cell proliferation, morphology, and spreading analyses. Laser groups presented irregular-shaped cavities on its surface and a typical microstructured surface with large depressions (FEG-SEM). The contact angle for both laser groups was 0°, whereas for the polished groups was ≈ 77 and ≈ 78 for cpTi-pol and Ti-15Mo-pol, respectively. Cell proliferation analysis demonstrated a higher metabolic activity in the laser groups (p < 0.05). From the fluorescence microscopy, Ti-15Mo-L surface seems to induce greater cellular differentiation compared to the cpTi-L surface. The preliminary biological in vitro analyses suggested possible advantages of laser surface treatment in the Ti-15Mo alloy regarding cell proliferation and maturation.

Keywords

Dental implants Surface modification Titanium alloys Cell culture SEM 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

Not required.

Informed consent

Not required.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Diagnosis and Surgery, School of Dentistry at AraraquaraSao Paulo State University-UNESPAraraquaraBrazil
  2. 2.Interfacial Electrochemistry Laboratory, Institute of Chemistry of São CarlosUSPSão CarlosBrazil
  3. 3.Department of Physical Chemistry, Institute of Chemistry of AraraquaraUNESPAraraquaraBrazil
  4. 4.Departamento de Diagnóstico e CirurgiaFaculdade de Odontologia de Araraquara – UNESPAraraquaraBrazil

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