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Improved production of lipid contents by cultivating Chlorella pyrenoidosa in heterogeneous organic substrates

  • Hesam KamyabEmail author
  • Shreeshivadasan Chelliapan
  • Chew Tin Lee
  • Tayebeh Khademi
  • Ashok Kumar
  • Krishna Kumar Yadav
  • Shahabaldin Rezania
  • Sandeep Kumar
  • Shirin Shafiei Ebrahimi
Original Paper
  • 24 Downloads

Abstract

The study is aimed to enhance the productivity of microalgal culture by varying the organic and inorganic components during wastewater treatment. A model organism Chlorella pyrenoidosa (C. pyrenoidosa) was grown in four different sources of wastewater namely piggery, palm oil mill effluent (POME), mixed-kitchen, and domestic wastes. The growth efficacy of C. pyrenoidosa on POME was tested for their ability to remove nutrients. It was observed that POME showed the highest chemical oxygen demand of 700 mg L−1. Meanwhile, the piggery waste had the highest amount of total nitrogen of 590 mg L−1. C. pyrenoidosa species were reported to grow well with different nutrient sources and produce high levels of lipids. The highest content of chlorophyll a was obtained with POME (3 mg L−1) and domestic wastes (2.5 mg L−1). The optimum growth rate of C. pyrenoidosa was reported for POME as a substrate. Also, the results indicated the lipid content for POME (182 mg L−1), domestic sample (148 mg L−1), piggery (0.99 mg L−1), and mixed-kitchen wastes (117 mg L−1). The results above revealed that among the tested substrates, POME could be the best alternative for C. pyrenoidosa to improve the yield of lipids and ultimately, biofuels production. Therefore, the treatment of POME in wastewater using C. pyrenoidosa can boost clean technology and energy generation. In future studies, the screening of other waste effluents is needed to cultivate the microalgae and enhance biomass production to meet increasing energy demands and waste treatment applications.

Keywords

Chlorella pyrenoidosa Lipid content Organic substrate Wastewater 

Notes

Acknowledgements

The authors would like to thank anonymous referees and editors for their constructive comments on the initial draft of the article. Also, the authors are grateful to IPASA, RAZAK School, and MJIT at Universiti Teknologi Malaysia (UTM) for providing adequate facilities to conduct this research; as well as Algaetech Sdn. Bhd. for their generous support. The first author is a researcher of UTM under the Post-Doctoral Fellowship Scheme (PDRU Grant) for the project: “Enhancing the lipid growth in algae cultivation for biodiesel production with Vote Number: Q. J130000.21A2.03E95 and Fundamental Research Grant Scheme (FRGS) with Vote Number: R. K130000.7856.5F049”. The authors would also like to acknowledge the supports from Dr.Amirreza Talaeikhozani, Mr.Rahim Kamyab and Mr.Parsa Kamyab in the research.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

References

  1. Abou-Shanab RA, Ji MK, Kim HC et al (2013) Microalgal species growing on piggery wastewater as a valuable candidate for nutrient removal and biodiesel production. J Environ Manag 115:257–264CrossRefGoogle Scholar
  2. Apha A (1995) WPCF Standard methods for the examination of water and wastewater. American Public Health Association/American Water Works Association/Water Environment Federation, WashingtonGoogle Scholar
  3. Avagyan AB (2008) A contribution to global sustainable development: inclusion of microalgae and their biomass in production and bio cycles. Clean Technol Environ Policy 10:313–317CrossRefGoogle Scholar
  4. Avagyan AB (2011) Water global recourse management through the use of microalgae addressed to sustainable development. Clean Technol Environ Policy 13:431–445CrossRefGoogle Scholar
  5. Avagyan AB (2018) Algae to energy and sustainable development. Technologies, resources, economics and system analyses. New Design of Global Environmental Policy and Live Conserve Industry. Amazon, ISBN-13: 978–1718722552, ISBN-10: 1718722559, 220pGoogle Scholar
  6. Bertanza G, Canato M, Laera G (2018) Towards energy self-sufficiency and integral material recovery in wastewater treatment plants: assessment of upgrading options. J Cleaner Prod 170:1206–1218CrossRefGoogle Scholar
  7. Beuckels A, Smolders E, Muylaert K (2015) Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Res 77:98–106CrossRefGoogle Scholar
  8. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  9. Cheirsilp B, Torpee S (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol 110:510–516CrossRefGoogle Scholar
  10. Chen CY, Yeh KL, Aisyah R et al (2011) Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresour Technol 102:71–81CrossRefGoogle Scholar
  11. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefGoogle Scholar
  12. Clesceri LS, Greenberg AE, Trussell RR (1989) Standard methods for the examination of water and wastewater. American Public Health Association Yearbook, WashingtonGoogle Scholar
  13. Cloern JE, Dufford R (2005) Phytoplankton community ecology: principles applied in San Francisco Bay. Mar Ecol Prog Ser 285:11–28CrossRefGoogle Scholar
  14. Converti A, Casazza A, Ortiz EY et al (2009) Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process Intensif 48:1146–1151CrossRefGoogle Scholar
  15. Costa JAV, Cozza KL, Oliveira L et al (2001) Different nitrogen sources and growth responses of Spirulina platensis in microenvironments. World J Microbiol Biotechnol 17:439–442CrossRefGoogle Scholar
  16. Crini G, Lichtfouse E (2019) Advantages and disadvantages of techniques used for wastewater treatment. Environ Chem Lett 17:145–155CrossRefGoogle Scholar
  17. El Asli A, El Mesbahi N, Oubakalla R et al (2019) Domestic wastewater treatment and lipid accumulation for biodiesel production by an isolated heterotrophic microalgae from an arid climate zone. Asia J Appl Microbiol 6:1–9CrossRefGoogle Scholar
  18. Ende S, Noke A (2019) Heterotrophic microalgae production on food waste and by-products. J Appl Phycol 31:1565–1571CrossRefGoogle Scholar
  19. Goh PS, Ong CS, Ng BC et al (2019) Applications of emerging nanomaterials for oily wastewater treatment. In: Nanotechnology water waste treat, pp 101–113.  https://doi.org/10.1016/B978-0-12-813902-8.00005-8
  20. Hossain MS, Omar F, Asis AJ et al (2019) Effective treatment of palm oil mill effluent using FeSO4·7H2O waste from titanium oxide industry: coagulation adsorption isotherm and kinetics studies. J Cleaner Prod 219:86–98CrossRefGoogle Scholar
  21. Huang Y, Cheng J, Lu H et al (2015) Simultaneous enhancement of microalgae biomass growth and lipid accumulation under continuous aeration with 15% CO2. RSC Adv 5:50851–50858CrossRefGoogle Scholar
  22. Ji MK, Kim HC, Sapireddy VR et al (2013) Simultaneous nutrient removal and lipid production from pretreated piggery wastewater by Chlorella vulgaris YSW-04. Appl Microbiol Biotechnol 97:2701–2710CrossRefGoogle Scholar
  23. Kamarudin KF, Tao DG, Yaakob Z et al (2015) A review on wastewater treatment and microalgal by-product production with a prospect of palm oil mill effluent (POME) utilization for algae. Der Pharma Chemica 7:73–89Google Scholar
  24. Kamyab H, Lim JS, Khademi T et al (2015) Greenhouse gas emission of organic waste composting: a case study of Universiti Teknologi Malaysia Green Campus Flagship Project. J Teknol 74:113–117Google Scholar
  25. Kamyab H, Din MFM, Ghoshal SK et al (2016a) Chlorella pyrenoidosa mediated lipid production using Malaysian agricultural wastewater: effects of photon and carbon. Waste Biomass Valoriz 7:779–788CrossRefGoogle Scholar
  26. Kamyab H, Din MFM, Hosseini SE et al (2016b) Optimum lipid production using agro-industrial wastewater treated microalgae as biofuel substrate. Clean Technol Environ Policy 18:2513–2523CrossRefGoogle Scholar
  27. Kamyab H, Md Din MF, Ponraj M et al (2016c) Isolation and screening of microalgae from agro-industrial wastewater (POME) for biomass and biodiesel sources. Desalin Water Treat 57:29118–29125CrossRefGoogle Scholar
  28. Kamyab H, Chelliapan S, Shahbazian-Yassar R, Din MFM, Khademi T, Kumar A, Rezania S (2017) Evaluation of lipid content in microalgae biomass using palm oil mill effluent (Pome). JOM 69:1361–1367CrossRefGoogle Scholar
  29. Kamyab H, Chelliapan S, Din MFM et al (2018a) Isolate new microalgal strain for biodiesel production and using FTIR spectroscopy for assessment of pollutant removal from palm oil mill effluent (POME). Chem Eng Trans 63:91–96Google Scholar
  30. Kamyab H, Chelliapan S, Lee CT et al (2018b) Microalgae cultivation using various sources of organic substrate for high lipid content. In: International conference on urban drainage modelling. Springer, Cham, pp 893–898Google Scholar
  31. Kothari R, Pathak VV, Kumar V et al (2012) Experimental study for growth potential of unicellular alga Chlorella pyrenoidosa on dairy wastewater: an integrated approach for treatment and biofuel production. Bioresour Technol 116:466–470CrossRefGoogle Scholar
  32. Lalucat J, Imperial J, Pares R (1984) Utilization of light for the assimilation of organic matter in Chlorella sp. VJ79. Biotechnol Bioeng 26:677–681CrossRefGoogle Scholar
  33. Lau PS, Tam NFY, Wong YS (1995) Effect of algal density on nutrient removal from primary settled wastewater. Environ Pollut 89:59–66CrossRefGoogle Scholar
  34. Li Y, Chen YF, Chen P et al (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102:5138–5144CrossRefGoogle Scholar
  35. Liu AY, Wei CHEN, Zheng L et al (2011) Identification of high-lipid producers for biodiesel production from forty-three green algal isolates in China. Prog Nat Sci Mater Int 21:269–276CrossRefGoogle Scholar
  36. Neoh CH, Lam CY, Ghani SM et al (2016) Bioremediation of high-strength agricultural wastewater using Ochrobactrum sp. strain SZ1. 3 Biotech 6:143CrossRefGoogle Scholar
  37. Órpez R, Martínez ME, Hodaifa G et al (2009) Growth of the microalga Botryococcus braunii in secondarily treated sewage. Desalination 246:625–630CrossRefGoogle Scholar
  38. Osundeko O, Ansolia P, Gupta SK et al (2019) Promises and challenges of growing microalgae in wastewater. In: Water conservation, recycling and reuse: issues and challenges. Springer, Singapore, pp 29–53Google Scholar
  39. Procházková G, Brányiková I, Zachleder V et al (2014) Effect of nutrient supply status on biomass composition of eukaryotic green microalgae. J Appl Phycol 26:1359–1377CrossRefGoogle Scholar
  40. Raeisossadati M, Vadiveloo A, Bahri PA et al (2019) Treating anaerobically digested piggery effluent (ADPE) using microalgae in thin layer reactor and raceway pond. J Appl Phycol 1–9Google Scholar
  41. Rai MP, Nigam S, Sharma R (2013) Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application. Biomass Bioenergy 58:251–257CrossRefGoogle Scholar
  42. Rao AR, Dayananda C, Sarada R et al (2007) Effect of salinity on growth of green alga Botryococcus braunii and its constituents. Bioresour Technol 98:560–564CrossRefGoogle Scholar
  43. Resdi R, Lim JS, Kamyab H et al (2016) Review of microalgae growth in palm oil mill effluent for lipid production. Clean Technol Environ Policy 18:2347–2361CrossRefGoogle Scholar
  44. Rezania S, Din MFM, Taib SM et al (2016) The efficient role of aquatic plant (water hyacinth) in treating domestic wastewater in continuous system. Int J Phytorem 18:679–685CrossRefGoogle Scholar
  45. Shelef G (2018) The engineering of microalgae mass cultures for treatment of agricultural wastewater, with special emphasis on selenium removal from drainage waters. In: Huntley ME (ed) Biotreatment of agricultural wastewater. CRC Press, Boca Raton, pp 143–148CrossRefGoogle Scholar
  46. Tan YD, Lim JS (2019) Feasibility of palm oil mill effluent elimination towards sustainable Malaysian palm oil industry. Renew Sustain Energy Rev 111:507–522CrossRefGoogle Scholar
  47. Vo HNP, Ngo H, Guo W et al (2019) Identification of the pollutants’ removal and mechanism by microalgae in saline wastewater. Bioresour Technol 275:44–52CrossRefGoogle Scholar
  48. Wang L, Min M, Li Y et al (2010) Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl Biochem Biotechnol 162:1174–1186CrossRefGoogle Scholar
  49. Wang H, Xiong Hui Z et al (2012) Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresour Technol 104:215–220CrossRefGoogle Scholar
  50. Widjaja A, Chien C, Ju YH (2009) Study of increasing lipid production from freshwater microalgae Chlorella vulgaris. J Taiwan Inst Chem Eng 40:13–20CrossRefGoogle Scholar
  51. Wu TY, Mohammad AW, Jahim JM et al (2009) A holistic approach to managing palm oil mill effluent (POME): biotechnological advances in the sustainable reuse of POME. Biotechnol Adv 27:40–52CrossRefGoogle Scholar
  52. Yang C, Hua Q, Shimizu K (2000) Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions. Biochem Eng J 6:87–102CrossRefGoogle Scholar
  53. Yoon JH, Shin JH, Ahn EK et al (2008) High cell density culture of Anabaena variabilis with controlled light intensity and nutrient supply. J Microbiol Biotechnol 18:918–925Google Scholar
  54. Yu H, Kim J, Lee C (2019) Nutrient removal and microalgal biomass production from different anaerobic digestion effluents with Chlorella species. Sci Rep 9:6123CrossRefGoogle Scholar
  55. Zabed HM, Akter S, Yun J et al (2019) Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production. Renew Sustain Energy Rev 105:105–128CrossRefGoogle Scholar
  56. Zainal BS, Zinatizadeh AA, Chyuan OH et al (2018) Effects of process, operational and environmental variables on biohydrogen production using palm oil mill effluent (POME). Int J Hydrog Energy 43:10637–10644CrossRefGoogle Scholar
  57. Zhu L, Wang Z, Shu Q et al (2013) Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Res 47:4294–4302CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Hesam Kamyab
    • 1
    • 2
    Email author
  • Shreeshivadasan Chelliapan
    • 1
  • Chew Tin Lee
    • 3
    • 4
  • Tayebeh Khademi
    • 5
  • Ashok Kumar
    • 6
  • Krishna Kumar Yadav
    • 7
  • Shahabaldin Rezania
    • 8
  • Sandeep Kumar
    • 9
  • Shirin Shafiei Ebrahimi
    • 10
  1. 1.Engineering Department, Razak Faculty of Technology and InformaticsUniversiti Teknologi MalaysiaKuala LumpurMalaysia
  2. 2.Department of Mechanical and Industrial EngineeringUniversity of Illinois at ChicagoChicagoUSA
  3. 3.School of Chemical and Energy EngineeringUniversiti Teknologi MalaysiaSkudaiMalaysia
  4. 4.Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA) PagohUniversiti Teknologi MalaysiaPagohMalaysia
  5. 5.Azman Hashim International Business SchoolUniversiti Teknologi Malaysia (UTM)SkudaiMalaysia
  6. 6.Department of Biotechnology and BioinformaticsJaypee University of Information TechnologyWaknaghat, SolanIndia
  7. 7.Institute of Environment and Development StudiesBundelkhand UniversityJhansiIndia
  8. 8.Department of Environment and EnergySejong University, Seoul 05006SeoulRepublic of Korea
  9. 9.Centre for Environment Science and Climate Resilient AgricultureIndian Agricultural Research InstituteNew DelhiIndia
  10. 10.School of EducationUniversiti Teknologi MalaysiaJohor BahruMalaysia

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