Could aflatoxin B1 production by Aspergillus flavus affect the severity of keratitis: an experience in two tertiary health care centers, Egypt
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To investigate the expression of AFB1 gene in isolates obtained from corneal scrapping samples from keratitis patients and to correlate the quantity of AFB1 to the severity of keratitis. An observational study was undertaken in Medical Microbiology and Immunology department, Mansoura University, Egypt, over corneal scrapping samples that were cultured aiming to isolate fungal causes of infective keratitis followed by AFB1 gene detection in Aspergillus flavus isolates by nested PCR then quantitation of the toxin by TLC. Out of 843 corneal scrapping samples collected from patients with infective keratitis, positive fungal growth was identified in 277 cases (32.9%). A. flavus was the commonest fungal agent isolated in 93 cases (33.6%). The AFB1 toxin–encoding gene was detected in 63.4% of A. flavus isolates. There was a positive correlation between the quantity of produced AFB1 toxin and the degree of severity of keratitis (P value < 0.0001*). Aspergillus flavus was the most common cause of fungal keratitis, with the AFB1 toxin–encoding gene detected in more than half of the isolates. A significant correlation between the degree of severity of keratitis and the quantity of produced AFB1 toxin was detected. Therefore, exploring presence or absence of AFB1 toxin is important for the clinicians in their diagnostic assessment and selection of proper treatment choices.
KeywordsA. flavus Aflatoxin B1 Fungal keratitis Nested PCR Thin-line chromatography
- A. flavus
- Nested PCR
Nested polymerase chain reaction
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The study protocol acquired an approval from our institutional review board (MS.18.11.360).
An informative consent was received from each study participant.
- 7.Hara S, Murakami H, Ohba T, Sugama S (1973) Studies on the mycological characters of aflatoxin-producing strains belonging to the A. flavus group. E. Production of moldy rice containing aflatoxin from rice and saki brewing with the moldy rice. Rep Res Inst Brew 145:13–18Google Scholar
- 24.Selvam et al. (2014) Biosci Biotech Res Asia 11(2):479–490Google Scholar
- 28.Shri K, Srivastava D, Mishra RN (1984) Toxic effects of aflatoxins on eyes – an experimental clinic-histopathological evaluation. Indian J Ophthalmol 32:242–246Google Scholar