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Multi-step optimization of the filtration method for the isolation of Campylobacter species from stool samples

  • Anne TilmanneEmail author
  • Helga Marisca Kandet Yattara
  • Margaux Herpol
  • Linda Vlaes
  • Patricia Retore
  • Caroline Quach
  • Olivier Vandenberg
  • Marie Hallin
  • Delphine Martiny
Original Article
  • 19 Downloads

Abstract

The filtration method (FM) is the most effective isolation technique for Epsilobacteriaceae from stool samples. FM’s different adaptations make it difficult to compare data between studies. This study was performed in three phases to optimize FM from a routine laboratory perspective. In July–September 2014 (part I), FM was performed on Mueller–Hinton agar containing 5% sheep blood and Columbia agar containing 5% sheep blood. In July 2016 (part II), FM was performed using 0.60-μm pore size polycarbonate filters (0.6-PC filter) and 0.45-μm pore size cellulose acetate filters (0.45-AC filter); in January 2018 (part III), the addition of hydrogen to incubators was studied. On 1146 stools analyzed in part I, the positive samples that showed no growth on the Butzler medium (n = 22/72, 30.6%) had improved growth of Epsilobacteriaceae when using the Columbia instead of the Mueller–Hinton medium (21/22 strains vs. 11/22, p < 0.05). In part II, on 718 stools, 91 strains grew with FM (12.7%), more with 0.6-PC filter (90/91) than with 0.45-AC filter (44/91) (p < 0.05). In part III, 578 stools were cultured, 98 Epsilobacteriaceae strains grew with FM, and 7% hydrogen finding significantly more Epsilobacteriaceae than without hydrogen (90/98, 91.8%, vs. 72/98, 73.5%; p < 0.05). The use of a Columbia medium containing 5% sheep blood with 0.6-PC filters incubated at 37 °C in a 7% hydrogen-enriched atmosphere led to an almost fourfold increase in the isolation rate of Epsilobacteriaceae among the studied combinations. Reference centers for Campylobacter should use standardized protocols to enable the comparison of prevalence in space and time.

Keywords

Campylobacter Filtration method Filter Hydrogen Concisus Gastroenteritis 

Notes

Acknowledgments

This work was supported by The Belgian Kids’ Fund for Pediatric Research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed were in accordance with the ethical standards of our institutional research committee.

Informed consent

Data were totally anonymized before analysis, no consent had to be obtained considering the methodology of the present work.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Anne Tilmanne
    • 1
    • 2
    Email author
  • Helga Marisca Kandet Yattara
    • 3
  • Margaux Herpol
    • 3
    • 4
  • Linda Vlaes
    • 3
    • 4
  • Patricia Retore
    • 3
    • 4
  • Caroline Quach
    • 2
    • 5
  • Olivier Vandenberg
    • 4
    • 6
    • 7
  • Marie Hallin
    • 3
  • Delphine Martiny
    • 3
    • 4
    • 8
  1. 1.Division of Infection Prevention and ControlHôpital Universitaire des Enfants Reine FabiolaBrusselsBelgium
  2. 2.Division of Pediatric Infectious DiseasesCHU Sainte JustineMontrealCanada
  3. 3.Department of MicrobiologyLaboratoire Hospitalier Universitaire de Bruxelles – Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles (ULB)BrusselsBelgium
  4. 4.National Reference Centre for CampylobacterCHU Saint-PierreBrusselsBelgium
  5. 5.Department of Microbiology, Infectious Diseases & ImmunologyUniversité de MontréalMontrealCanada
  6. 6.Innovation and Business Development Unit, LHUB-ULBPole Hospitalier Universitaire de Bruxelles, Université Libre de BruxellesBrusselsBelgium
  7. 7.Centre for Environmental Health and Occupational Health, School of Public HealthUniversité Libre de Bruxelles (ULB)BrusselsBelgium
  8. 8.Faculté de Médecine et PharmacieUniversité de Mons (UMONS)MonsBelgium

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