Role of prolonged blood culture incubation in infective endocarditis diagnosis

  • Madiha FidaEmail author
  • Brenda L. Dylla
  • M. Rizwan Sohail
  • Bobbi S. Pritt
  • Audrey N. Schuetz
  • Robin Patel
Letter to the Editor

We read with interest the recent publication by Lindell et al. describing a large cohort of Cutibacterium/Propionibacterium prosthetic valve endocarditis (PVE) [1]. Cutibacterium/Propioniobacterium species are facultative anaerobes which are part of the normal skin microbiota and are increasingly recognized as a cause of endovascular infection, in recent reports, constituting 3.8–8% of PVE cases [1, 2]. As slow-growing organisms, Cutibacterium/Propionibacteriumspecies may require prolonged durations of incubation for isolation. Accordingly, they may be missed with conventional durations of blood culture incubation. Prolonged blood culture incubation is frequently requested by the Infectious Diseases clinicians at our institution in cases of suspected endocarditis; however, the clinical value of this practice has been incompletely defined. Likewise, the clinical value of blind subcultures and terminal Gram or acridine orange staining of negative blood culture bottles in such...


  1. 1.
    Lindell F et al (2018) Prosthetic valve endocarditis caused by Propionibacterium species: a national registry-based study of 51 Swedish cases. Eur J Clin Microbiol Infect Dis 37(4):765–771CrossRefGoogle Scholar
  2. 2.
    Banzon JM et al (2017) Propionibacterium acnes endocarditis: a case series. Clin Microbiol Infect 23(6):396–399CrossRefGoogle Scholar
  3. 3.
    CLSI (2007) Principles and procedures for blood cultures; Approved Guideline. CLSI document M47-A. Clinical and Laboratory Standards Institute, Wayne, p 67Google Scholar
  4. 4.
    Sohail MR et al (2009) Infective endocarditis due to Propionibacterium species. Clin Microbiol Infect 15(4):387–394CrossRefGoogle Scholar
  5. 5.
    Piper KE et al (2009) Microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication. J Clin Microbiol 47(6):1878–1884CrossRefGoogle Scholar
  6. 6.
    Baron EJ, Scott JD, Tompkins LS (2005) Prolonged incubation and extensive subculturing do not increase recovery of clinically significant microorganisms from standard automated blood cultures. Clin Infect Dis 41(11):1677–1680CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Infectious Diseases, Department of Internal MedicineMayo ClinicRochesterUSA
  2. 2.Division of Clinical Microbiology, Department of Laboratory Medicine and PathologyMayo ClinicRochesterUSA

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