Role of prolonged blood culture incubation in infective endocarditis diagnosis
We read with interest the recent publication by Lindell et al. describing a large cohort of Cutibacterium/Propionibacterium prosthetic valve endocarditis (PVE) . Cutibacterium/Propioniobacterium species are facultative anaerobes which are part of the normal skin microbiota and are increasingly recognized as a cause of endovascular infection, in recent reports, constituting 3.8–8% of PVE cases [1, 2]. As slow-growing organisms, Cutibacterium/Propionibacteriumspecies may require prolonged durations of incubation for isolation. Accordingly, they may be missed with conventional durations of blood culture incubation. Prolonged blood culture incubation is frequently requested by the Infectious Diseases clinicians at our institution in cases of suspected endocarditis; however, the clinical value of this practice has been incompletely defined. Likewise, the clinical value of blind subcultures and terminal Gram or acridine orange staining of negative blood culture bottles in such...
- 3.CLSI (2007) Principles and procedures for blood cultures; Approved Guideline. CLSI document M47-A. Clinical and Laboratory Standards Institute, Wayne, p 67Google Scholar