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Detection of toxoplasma-specific immunoglobulin G in human sera: performance comparison of in house Dot-ELISA with ECLIA and ELISA

  • Aref Teimouri
  • Mohammad Hossein Modarressi
  • Saeedeh Shojaee
  • Mehdi Mohebali
  • Nima Zouei
  • Mostafa Rezaian
  • Hossein Keshavarz
Original Article

Abstract

In the current study, performance of electrochemiluminescence immunoassay (ECLIA) in detection of anti-toxoplasma IgG in human sera was compared with that of enzyme-linked immunosorbent assay (ELISA). Furthermore, performance of an in house Dot-ELISA in detection of anti-toxoplasma IgG was compared with that of ECLIA and ELISA. In total, 219 human sera were tested to detect anti-toxoplasma IgG using Dynex DS2® and Roche Cobas® e411 Automated Analyzers. Discordant results rechecked using immunofluorescence assay (IFA). Then, sera were used in an in house Dot-ELISA to assess toxoplasma-specific IgG. Of the 219 samples, two samples were found undetermined using ECLIA but reactive using ELISA. Using IFA, the two sera were reported unreactive. Furthermore, two samples were found reactive using ECLIA and unreactive using ELISA. These samples were reported reactive using IFA. The overall agreement for the two former methods was 98% (rZ0.98.1; P < 0.001). The intrinsic parameters calculated for in house Dot-ELISA included sensitivity of 79.5, specificity of 78.2, and accuracy of 78.9%, compared to ECLIA and ELISA. Positive and negative predictive values included 82.9 and 74.2%, respectively. A 100% sensitivity was found in in house Dot-ELISA for highly reactive sera in ECLIA and ELISA. ECLIA is appropriate for the first-line serological screening tests and can replace ELISA due to high speed, sensitivity, and specificity, particularly in large laboratories. Dot-ELISA is a rapid, sensitive, specific, cost-effective, user-friendly, and field-portable technique and hence can be used for screening toxoplasmosis, especially in rural fields or less equipped laboratories.

Keywords

Anti-toxoplasma IgG In house Dot-ELISA ECLIA ELISA 

Abbreviations

ECLIA

electrochemiluminescence immunoassay

ELISA

enzyme-linked immunosorbent assay

IgG

immunoglobulin G

DT

dye test

IFA

immunofluorescence assay

CFT

complement fixation test

MAT

modified agglutination test

LAT

latex agglutination test

DAT

direct agglutination test

IFAT

indirect fluorescent antibody test

IHA

indirect hemagglutination assay

PBS-M 3%

phosphate-buffered saline containing 3% of non-fat dry milk

PBS-T

phosphate-buffered saline containing tween 20

HRP

horseradish peroxidase

OPD

ortho phenylenediamine

OD

optical density

NC

nitrocellulose membrane

IBM

International Business Machines Corporation

SPSS

Statistical Package for the Social Sciences

Notes

Acknowledgements

We would like to acknowledge all staff from the toxoplasmosis laboratory (Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran) for their useful collaboration.

Authors’ contribution

HK and MHM conceived the study. NZ collected and prepared the samples. AT performed the experiments with substantial contributions by SS. AT drafted the manuscript and revised by SS, MM, and MR. All authors read and approved the final version of the manuscript.

Funding information

This study was supported by the Tehran University of Medical Sciences and Health Services (Project Number: 29999-160-03-94), Tehran, Iran.

Compliance with ethical standards

Ethics approval and consent to participate

The study was approved by the Ethical Committee of Tehran University of Medical Sciences.

Consent for publication

Not applicable (no individual person’s data).

Availability of data and material

The data that support the findings of this study are available on reasonable request to the corresponding author.

Competing interests

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Aref Teimouri
    • 1
    • 2
  • Mohammad Hossein Modarressi
    • 3
  • Saeedeh Shojaee
    • 1
  • Mehdi Mohebali
    • 1
  • Nima Zouei
    • 1
  • Mostafa Rezaian
    • 1
  • Hossein Keshavarz
    • 1
  1. 1.Department of Medical Parasitology and Mycology, School of Public HealthTehran University of Medical SciencesTehranIran
  2. 2.Students Scientific Research CenterTehran University of Medical SciencesTehranIran
  3. 3.Department of Medical Genetics, School of MedicineTehran University of Medical SciencesTehranIran

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