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Evaluation of a rapid diagnostic test for detection of dengue infection using a single-tag hybridization chromatographic-printed array strip format

  • Veni R. Liles
  • Lady-Anne S. Pangilinan
  • Maria Luisa G. Daroy
  • Maria Terrese A. Dimamay
  • Rachel S. Reyes
  • Mick Kelvin Bulusan
  • Mark Pierre S. Dimamay
  • Paolo Antonio S. Luna
  • Abigail Mercado
  • Gaowa Bai
  • Haorile Chagan-Yasutan
  • Yutaka Takarada
  • Mitsuo Kawase
  • Toshio HattoriEmail author
Original Article
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Abstract

A dipstick DNA chromatography assay, a single-tag hybridization-printed array strip (STH-PAS), was evaluated for its efficacy to detect dengue virus (DENV). Reverse-transcribed DNA was amplified by PCR, and the amplified DNA was detected using the STH-PAS system. The method was evaluated using stored RNA samples previously identified to carry all 4 serotypes of dengue, chikungunya, and influenza viruses. Clinical performance was also assessed in a prospective study using plasma from 269 febrile cases from the Emergency Department of St. Luke’s Medical Center, Quezon City, Philippines, and 30 afebrile normal healthy volunteers. A Taqman real-time PCR (RT-PCR) assay and a rapid Dengue NS1 test, SD Bioline, were used for comparison. The STH-PAS system was more sensitive in detecting dengue infection compared to Taqman RT-PCR. For DENV serotypes 1, 2, and 3, the detection was 1 to 2 dilutions (10-fold) higher, and for DENV serotype 4, the detection was 2–4 dilutions higher. In clinical studies, the STH-PAS system showed 100% sensitivity with 88.9% and 86.6% specificities compared to Taqman RT-PCR and SD Dengue Duo NS1 test, respectively. The STH-PAS system was found to have a superior sensitivity than the Taqman system. Further evaluation of its performance in the field may provide important data to extend its usefulness for surveillance and epidemiological research in outbreak situations.

Keywords

Dengue Rapid diagnostics Polymerase chain reaction STH-PAS 

Notes

Acknowledgements

We acknowledge the valuable assistance of Sheena Marie Asur in patient recruitment.

Authors’ contributions

Conceived and designed the experiments: YT, TH, MK, HCY, MLGD, MTAD.

Performed the experiments: VRL, LASP, RSR, MKB.

Analyzed the data: VRL, LASP, RSR, MLGD, MTAD, MPSD, YT, TH, MK, HCY.

Contributed reagents/materials/analysis tools: MK, YT, TH, PASL, AM, GB.

Wrote the paper: MLGD, VRL, LASP, HCY, TH.

Contributed to the logistics of kits: YT, TH.

Funding

This research was financially supported by Tohoku Bureau of Economy, Trade, and Industry and the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) grant number JP17H01690.

Compliance with ethical standards

The study was approved by the Institutional Ethics Review Committee, EC No. CT-17010, and conducted in St. Luke’s Medical Center, Quezon City, Philippines.

Competing interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496(7446):504–507CrossRefGoogle Scholar
  2. 2.
    Brady OJ, Gething PW, Bhatt S, Messina JP, Brownstein JS, Hoen AG, Moyes CL, Farlow AW, Scott TW, Hay SI (2012) Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl Trop Dis 6(8):e1760CrossRefGoogle Scholar
  3. 3.
    Simmons CP, Farrar JJ, Nguyen V, Wills B (2012) Dengue. N Engl J Med 366(15):1423–1432CrossRefGoogle Scholar
  4. 4.
    World Health Organization (2009) Dengue. Guidelines for diagnosis, treatment, prevention and control. http://www.who.int/tdr/publications/documents/dengue-diagnosis.pdf Accessed 01 Oct 2018
  5. 5.
    Hunsperger EA, Yoksan S, Buchy P, Nguyen VC, Sekaran SD, Enria DA, Vazquez S, Cartozian E, Pelegrino JL, Artsob H, Guzman MG, Olliaro P, Zwang J, Guillerm M, Kliks S, Halstead S, Peeling RW, Margolis HS (2014) Evaluation of commercially available diagnostic tests for the detection of dengue virus NS1 antigen and anti-dengue virus IgM antibody. PLoS Negl Trop Dis 8(10):e3171CrossRefGoogle Scholar
  6. 6.
    Pal S, Dauner AL, Valks A, Forshey BM, Long KC, Thaisomboonsuk B, Sierra G, Picos V, Talmage S, Morrison AC, Halsey ES, Comach G, Yasuda C, Loeffelholz M, Jarman RG, Fernandez S, An US, Kochel TJ, Jasper LE, Wu SJ (2015) Multicountry prospective clinical evaluation of two enzyme-linked immunosorbent assays and two rapid diagnostic tests for diagnosing dengue fever. J Clin Microbiol 53(4):1092–1102CrossRefGoogle Scholar
  7. 7.
    Alm E, Lesko B, Lindegren G, Ahlm C, Soderholm S, Falk KI, Lagerqvist N (2014) Universal single-probe RT-PCR assay for diagnosis of dengue virus infections. PLoS Negl Trop Dis 8(12):e3416CrossRefGoogle Scholar
  8. 8.
    Hayashi M, Natori T, Kubota-Hayashi S, Miyata M, Ohkusu K, Kawamoto K, Kurazono H, Makino S, Ezaki T (2013) A new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth. Biomed Res Int 2013:295050Google Scholar
  9. 9.
    Tian L, Sato T, Niwa K, Kawase M, Tanner AC, Takahashi N (2014) Rapid and sensitive PCR-dipstick DNA chromatography for multiplex analysis of the oral microbiota. Biomed Res Int 2014:180323Google Scholar
  10. 10.
    Koiwai K, Kodera T, Thawonsuwan J, Kawase M, Kondo H, Hirono I (2018) A rapid method for simultaneously diagnosing four shrimp diseases using PCR-DNA chromatography method. J Fish Dis 41(2):395–399CrossRefGoogle Scholar
  11. 11.
    Koiwai K, Kodera T, Thawonsuwan J, Riani S, Kawase M, Kondo H, Hirono I (2018) Rapid diagnosis of three shrimp RNA viruses using RT-PCR-DNA chromatography. J Fish Dis 41(8):1309–1312.  https://doi.org/10.1111/jfd.12821 CrossRefGoogle Scholar
  12. 12.
    Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV (1992) Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30(3):545–551Google Scholar
  13. 13.
    Furuya-Kanamori L, Liang S, Milinovich G, Soares Magalhaes RJ, Clements AC, Hu W, Brasil P, Frentiu FD, Dunning R, Yakob L (2016) Co-distribution and co-infection of chikungunya and dengue viruses. BMC Infect Dis 16:84CrossRefGoogle Scholar
  14. 14.
    Salam N, Mustafa S, Hafiz A, Chaudhary AA, Deeba F, Parveen S (2018) Global prevalence and distribution of coinfection of malaria, dengue and chikungunya: a systematic review. BMC Public Health 18(1):710CrossRefGoogle Scholar
  15. 15.
    Villamil-Gomez WE, Rodriguez-Morales AJ, Uribe-Garcia AM, Gonzalez-Arismendy E, Castellanos JE, Calvo EP, Alvarez-Mon M, Musso D (2016) Zika, dengue, and chikungunya co-infection in a pregnant woman from Colombia. Int J Infect Dis 51:135–138CrossRefGoogle Scholar
  16. 16.
    Carter MJ, Emary KR, Moore CE, Parry CM, Sona S, Putchhat H, Reaksmey S, Chanpheaktra N, Stoesser N, Dobson AD, Day NP, Kumar V, Blacksell SD (2016) Correction: rapid diagnostic tests for dengue virus infection in febrile Cambodian children: diagnostic accuracy and incorporation into diagnostic algorithms. PLoS Negl Trop Dis 10(2):e0004453CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Veni R. Liles
    • 1
  • Lady-Anne S. Pangilinan
    • 1
  • Maria Luisa G. Daroy
    • 1
  • Maria Terrese A. Dimamay
    • 1
  • Rachel S. Reyes
    • 1
  • Mick Kelvin Bulusan
    • 1
  • Mark Pierre S. Dimamay
    • 1
  • Paolo Antonio S. Luna
    • 2
  • Abigail Mercado
    • 2
  • Gaowa Bai
    • 3
  • Haorile Chagan-Yasutan
    • 3
    • 4
  • Yutaka Takarada
    • 5
  • Mitsuo Kawase
    • 5
    • 6
  • Toshio Hattori
    • 3
    Email author
  1. 1.Research and Biotechnology GroupSt. Luke’s Medical CenterQuezon CityPhilippines
  2. 2.Department of Emergency MedicineSt. Luke’s Medical CenterQuezon CityPhilippines
  3. 3.Department of Health Science and Social WelfareKibi International UniversityTakahashiJapan
  4. 4.Mongolian Psychosomatic Medicine DepartmentInternational Mongolian Medicine Hospital of Inner MongoliaHohhotChina
  5. 5.Tohoku Bio-Array Co., Ltd.SendaiJapan
  6. 6.Graduate School of Biomedical EngineeringTohoku UniversitySendaiJapan

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