Advertisement

Comparison between Vitek MS, Bruker Biotyper, Vitek2, and API20E for differentiation of species of the genus Raoultella

  • Carlos Ruiz de Alegría PuigEmail author
  • Marina Fernández Torres
  • Eduardo Marfil-Pérez
  • María Isabel Rodríguez Ferández
  • Manuel Causse Del Río
  • Jesús Agüero Balbín
  • Luis Martínez-Martínez
Original Article
  • 51 Downloads

Abstract

Rapid and reliable identification of microorganisms in the clinical laboratory is essential for an early and accurate diagnosis guiding timely therapy. However, conventional methods are sometimes unreliable and show controversial outcomes. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a rapid and reliable method for identification of bacteria and fungi isolated from clinical samples. Members of the genus Raoultella are increasingly recognized as clinically relevant. There are difficulties in their identification at the species level since sequencing the 16S rRNA or the rpoB genes does not show conclusive results. The aim of this study has been to compare two MALDI-TOF MS systems (Vitek MS and Bruker Biotyper) with Vitek2 and API20E systems for differentiation of Raoultella species. A collection of 97 clinical isolates of Raoultella species was identified with Vitek MS, in parallel with Vitek2 and API, and finally with Bruker Biotyper. Among the two most widely used MALDI-TOF MS platforms, results obtained with Vitek MS were slightly superior to those obtained with the Bruker Biotyper system, with sensitivities and specificities of 98.9/57.9% and 98.8/37.0%, respectively. The current commercial phenotypic identification systems are not optimized for the identification of Raoultella species. Our results indicate that MALDI-TOF-based identification is more accurate and sensitive than that provided by phenotypic methods.

Keywords

MALDI-TOF MS Raoultella Vitek MS Bruker Biotyper 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This work has gone through the ethics committee of our hospital without receiving any kind of objection.

Informed consent

This work has not needed any informed consent.

References

  1. 1.
    Adeolu M, Alnajar S, Naushad SS, Gupta R (2016) Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol 66:5575–5599.  https://doi.org/10.1099/ijsem.0.001485 CrossRefGoogle Scholar
  2. 2.
    Drancourt M, Bollet C, Carta A, Rousselier P (2001) Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51:925–932.  https://doi.org/10.1099/00207713-51-3-925 CrossRefGoogle Scholar
  3. 3.
    Ulukent SC, Sarici İS, Alper Sahbaz N, Ozgun YM, Akca O, Sanlı K (2017) Is it necessary to specifically define the cause of surgically treated biliary tract infections? A rare case of Raoultella planticola cholecystitis and literature review. Case Rep Infect Dis 2017.  https://doi.org/10.1155/2017/4181582
  4. 4.
    Alves MS, Moreira BM, Riley LW (2007) A case of severe pancreatitis complicated by Raoultella planticola infection. J Med Microbiol 56:696–698.  https://doi.org/10.1099/jmm.0.46889-0 CrossRefGoogle Scholar
  5. 5.
    Seng P, Boushab BM, Romain F, Gouriet F, Bruder N, Martin C, Paganelli F, Bernit E, Le Treut YP, Thomas P, Papazian L, Raoult D, Stein A (2016) Emerging role of Raoultella ornithinolytica in human infections: a series of cases and review of the literature. Int J Infect Dis 45:65–71.  https://doi.org/10.1016/j.ijid.2016.02.014 CrossRefGoogle Scholar
  6. 6.
    Demiray T, Köroğlu M, Özbek A, Hafizoğlu T, Altındiş M (2015) The first case of Raoultella terrigena infection in an infant. Turk J Pediatr 57(6):624–628Google Scholar
  7. 7.
    Koukoulaki M, Bakalis A, Kalatzis V, Belesiotou E, Papastamopoulos V, Skoutelis A, Drakopoulos S (2014) Acute prostatitis caused by Raoultella planticola in a renal transplant recipient: a novel case. Transpl Infect Dis 16(3):461–464.  https://doi.org/10.1111/tid.12213 CrossRefGoogle Scholar
  8. 8.
    Kalaria SS, Elliott K, Combs N, Phillips LG (2017) Raoultella planticola: a rare cause of wound infection. Wounds 29(11):E103–E105Google Scholar
  9. 9.
    O' Connell K, Kelly J, Niriain U (2010) A rare case of soft-tissue infection caused by Raoultella planticola. Case Rep Med 2010.  https://doi.org/10.1155/2010/134086
  10. 10.
    Jain AK, Yadav R (2017) First report of isolation and antibiotic susceptibility pattern of Raoultella electrica from table eggs in Jaipur, India. New Microbes New Infect 21:95–99.  https://doi.org/10.1016/j.nmni.2017.11.004 CrossRefGoogle Scholar
  11. 11.
    Chun S, Yun JW, Huh HJ, Lee NY (2015) Clinical characteristics of Raoultella ornithinolytica bacteremia. Infection 43:59–64.  https://doi.org/10.1007/s15010-014-0696-z CrossRefGoogle Scholar
  12. 12.
    Chun S, Yun JW, Huh HJ, Lee NY (2014) Low virulence? Clinical characteristics of Raoultella planticola bacteremia. Infection 42:899–904.  https://doi.org/10.1007/s15010-014-0664-7 CrossRefGoogle Scholar
  13. 13.
    Park JS, Hong KH, Lee HJ, Choi SH, Song SH, Song KH, Kim HB, Park KU, Song J, Kim EC (2011) Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica. J Med Microbiol 60(4):492–499.  https://doi.org/10.1099/jmm.0.020768-0 CrossRefGoogle Scholar
  14. 14.
    Ponce-Alonso M, Rodríguez-Rojas L, Del Campo R, Cantón R, Morosini MI (2016) Comparison of different methods for identification of species of the genus Raoultella: report of 11 cases of Raoultella causing bacteremia and literature review. Clin Microbiol Infect 22(3):252–257.  https://doi.org/10.1016/j.cmi.2015.10.035 CrossRefGoogle Scholar
  15. 15.
    De Jong E, De Jong AS, Smidts-van den Berg N, Rentenaar RJ (2013) Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Diagn Microbiol Infect Dis 75(4):431–433.  https://doi.org/10.1016/j.diagmicrobio.2012.12.009 CrossRefGoogle Scholar
  16. 16.
    Kovtunovych G, Lytvynenko T, Negrutska V, Lar O, Brisse S, Kozyrovska N (2003) Identification of Klebsiella oxytoca using a specific PCR assay targeting the polygalacturonase pehX gene. Res Microbiol 154(8):587–592.  https://doi.org/10.1016/S0923-2508(03)00148-7 CrossRefGoogle Scholar
  17. 17.
    Walckenaer E, Leflon-Guibout V, Nicolas-Chanoine MH (2008) How to identify Raoultella spp. including R. ornithinolytica isolates negative for ornithine decarboxylase? The reliability of the chromosomal bla gene. J Microbiol Methods 75(3):405–410.  https://doi.org/10.1016/j.mimet.2008.07.011 CrossRefGoogle Scholar
  18. 18.
    Kimura Z, Chung KM, Itoh H, Hiraishi A, Okabe S (2014) Raoultella electrica sp. nov., isolated from anodic biofilms of a glucose-fed microbial fuel cell. Int J Syst Evol Microbiol 64:1384–1388.  https://doi.org/10.1099/ijs.0.058826-0 CrossRefGoogle Scholar
  19. 19.
    Patrick R. Murray et al. (2007) Manual of clinical microbiology. 9rd edn. Washington, D.C., pp 700–706Google Scholar
  20. 20.
    Richter SS, Sercia L, Branda JA, Burnham CA, Bythrow M, Ferraro MJ, Garner OB, Ginocchio CC, Jennemann R, Lewinski MA, Manji R, Mochon AB, Rychert JA, Westblade LF, Procop GW (2013) Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system. Eur J Clin Microbiol Infect Dis 32(12):1571–1578.  https://doi.org/10.1007/s10096-013-1912-y CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Carlos Ruiz de Alegría Puig
    • 1
    Email author
  • Marina Fernández Torres
    • 1
  • Eduardo Marfil-Pérez
    • 2
    • 3
  • María Isabel Rodríguez Ferández
    • 1
  • Manuel Causse Del Río
    • 2
    • 3
  • Jesús Agüero Balbín
    • 1
    • 4
  • Luis Martínez-Martínez
    • 2
    • 3
    • 5
  1. 1.Microbiology ServiceUniversity Hospital Marqués de Valdecilla-IDIVALSantanderSpain
  2. 2.Clinical UnitUniversity Hospital Reina SofíaCórdobaSpain
  3. 3.Maimónides Institute for Biomedical Research of Córdoba (IMIBIC)CórdobaSpain
  4. 4.Department of Molecular BiologyUniversity of CantabriaSantanderSpain
  5. 5.Department of MicrobiologyUniversity of CórdobaCórdobaSpain

Personalised recommendations