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Expression analysis of long non-coding RNAs and their target genes in multiple sclerosis patients

  • Maziar Ganji
  • Arezou SayadEmail author
  • Mir Davood Omrani
  • Shahram Arsang-Jang
  • Mehrdokht Mazdeh
  • Mohammad TaheriEmail author
Original Article

Abstract

Multiple sclerosis (MS) is a progressive chronic autoimmune-mediated disease. Recently, long non-coding RNAs (lncRNAs) are characterized to participate in the adjustment of immune responses. Here, we evaluated the expression levels of GSTT1-AS1 and IFNG-AS1 lncRNAs and their targets (TNF and IFNG, respectively) in Iranian MS patients.

In this case-control study, 50 relapsing-remitting MS patients and 50 healthy subjects were recruited. Expressions of GSTT1-AS1 and IFNG-AS1 lncRNAs, as well as TNF and IFNG genes, were assessed in their peripheral blood samples by SYBR Green-based Real-time quantitative PCR.

Expression levels of GSTT1-AS1 and IFNG-AS1 lncRNAs were both significantly downregulated (p values 0.032 and 0.013, respectively). On the other hand, the expression of TNF and IFNG showed increased levels, however, did not reach statistical significance after our analysis (p > 0.05). Spearman correlation analysis showed that GSTT1-AS1 had a significant positive moderate correlation with IFNG-AS1 (r = 0.541, p < 0.0001), IFNG (r = 0.329, p = 0.001), and TNF (r = 0.204, p = 0.041). Also, IFNG-AS1 revealed the same correlation with IFNG (r = 0.475, p < 0.0001) as well as TNF (r = 0.399, p < 0.0001). Furthermore, GSTT1-AS1 (r = 0.313, p = 0.027) and (IFNG r = 0.478, p < 0.0001) demonstrated a significant positive correlation with age at onset.

Briefly, the current study provided for the first time dysregulation of GSTT1-AS1 and IFNG-AS lncRNAs network in MS, which highlights the significant role of epigenetic pathways in this autoimmune disorder. Larger sample size and further investigation assays could shed light on the underlying mechanisms in this area of science.

Keywords

Multiple sclerosis IFNG TNF GSTT1-AS1 IFNG-AS 

Notes

Funding information

The present article is financially supported by “Research Department of the School of Medicine Shahid Beheshti University of Medical Sciences.”

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Fondazione Società Italiana di Neurologia 2019

Authors and Affiliations

  1. 1.Department of Medical GeneticsShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Department of Medical Genetics, School of MedicineShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Urogenital Stem Cell Research CenterShahid Beheshti University of Medical SciencesTehranIran
  4. 4.Clinical Research Development Center (CRDU)Qom University of Medical SciencesQomIran
  5. 5.Neurophysiology Research CenterHamadan University of Medical SciencesHamadanIran

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