ERCC6L2 rs591486 polymorphism and risk for amyotrophic lateral sclerosis in Greek population

  • Efthimios DardiotisEmail author
  • Emmanouil Karampinis
  • Vasileios Siokas
  • Athina-Maria Aloizou
  • Dimitrios Rikos
  • Styliani Ralli
  • Dimitra Papadimitriou
  • Dimitrios P. Bogdanos
  • Georgios M. Hadjigeorgiou
Original Article



Α number of genetic variants have been associated with amyotrophic lateral sclerosis (ALS). A recent study supports that rs591486 across the ERCC6L2 gene and exposure to pesticides seem to have a joint effect on the development of Parkinson’s disease, a disease which shares a few common characteristics with ALS.


To detect a possible contribution of rs591486 ERCC6L2 to ALS.


A total of 155 patients with ALS and 155 healthy controls were included in the study and genotyped for rs591486. Using logistic regression analyses (crude and adjusted for age and sex), rs591486 was tested for association with ALS risk. Subgroup analysis based on ALS site of onset was also performed. Cox regression analysis was applied in order for the effect of ERCC6L2 rs591486 on ALS age of onset to be tested.


Adjusted analysis showed that ERCC6L2 rs591486 was associated with an increased risk of ALS development, in dominant [odds ratio, OR (95% confidence interval, CI) 2.15 (1.04–4.46), p = 0.037] and over-dominant [OR (95%CI) = 1.91 (1.01–3.60), p = 0.043], modes. Subgroup analysis based on ALS site of onset revealed an association between ERCC6L2 rs591486 and ALS with limb onset. Results for Cox regression analysis indicated that G/A carriers had a lower age of ALS limb onset when compared to G/G carriers.


The current study provides preliminary indication for an implication of ERCC6L2 rs591486 in ALS development, as a possible genetic risk factor. These results possibly suggest that oxidative stress may be the main contributor in the pathophysiology of ALS.


ALS Oxidative stress Pesticides Polymorphism ERCC6L2 



The study was supported in part by a research grant of the Research Committee of the University of Thessaly, Greece (Code: 5287).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

Supplementary material

10072_2019_3825_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 15 kb)


  1. 1.
    Al-Chalabi A, Hardiman O, Kiernan MC, Chio A, Rix-Brooks B, van den Berg LH (2016) Amyotrophic lateral sclerosis: moving towards a new classification system. Lancet Neurol 15(11):1182–1194. CrossRefGoogle Scholar
  2. 2.
    Liu J, Zhang X, Ding X, Song M, Sui K (2019) Analysis of clinical and electrophysiological characteristics of 150 patients with amyotrophic lateral sclerosis in China. Neurol Sci 40(2):363–369. CrossRefGoogle Scholar
  3. 3.
    Tortarolo M, Lo Coco D, Veglianese P, Vallarola A, Giordana MT, Marcon G, Beghi E, Poloni M, Strong MJ, Iyer AM, Aronica E, Bendotti C (2017) Amyotrophic lateral sclerosis, a multisystem pathology: insights into the role of TNFalpha. Mediat Inflamm 2017:2985051. CrossRefGoogle Scholar
  4. 4.
    Dardiotis E, Siokas V, Sokratous M, Tsouris Z, Aloizou AM, Florou D, Dastamani M, Mentis AA, Brotis AG (2018) Body mass index and survival from amyotrophic lateral sclerosis: a meta-analysis. Neurol Clin Pract 8(5):437–444. CrossRefGoogle Scholar
  5. 5.
    Dardiotis E, Siokas V, Sokratous M, Tsouris Z, Michalopoulou A, Andravizou A, Dastamani M, Ralli S, Vinceti M, Tsatsakis A, Hadjigeorgiou GM (2018) Genetic polymorphisms in amyotrophic lateral sclerosis: evidence for implication in detoxification pathways of environmental toxicants. Environ Int 116:122–135. CrossRefGoogle Scholar
  6. 6.
    Chen Y, Zhou Q, Gu X, Wei Q, Cao B, Liu H, Hou Y, Shang H (2018) An association study between SCFD1 rs10139154 variant and amyotrophic lateral sclerosis in a Chinese cohort. Amyotroph Lateral Scler Frontotemporal Degener 19(5–6):413–418. CrossRefGoogle Scholar
  7. 7.
    Dardiotis E, Aloizou AM, Siokas V, Patrinos GP, Deretzi G, Mitsias P, Aschner M, Tsatsakis A (2018) The role of microRNAs in patients with amyotrophic lateral sclerosis. J Mol Neurosci 66(4):617–628. CrossRefGoogle Scholar
  8. 8.
    Cui R, Tuo M, Li P, Zhou C (2018) Association between TBK1 mutations and risk of amyotrophic lateral sclerosis/frontotemporal dementia spectrum: a meta-analysis. Neurol Sci 39(5):811–820. CrossRefGoogle Scholar
  9. 9.
    Ning P, Yang X, Yang B, Zhao Q, Huang H, An R, Chen Y, Hu F, Xu Z, Xu Y (2018) Meta-analysis of the association between ZNF512B polymorphism rs2275294 and risk of amyotrophic lateral sclerosis. Neurol Sci 39(7):1261–1266. CrossRefGoogle Scholar
  10. 10.
    Da Cruz S, Bui A, Saberi S, Lee SK, Stauffer J, McAlonis-Downes M, Schulte D, Pizzo DP, Parone PA, Cleveland DW, Ravits J (2017) Misfolded SOD1 is not a primary component of sporadic ALS. Acta Neuropathol 134(1):97–111. CrossRefGoogle Scholar
  11. 11.
    Turner MR, Bowser R, Bruijn L, Dupuis L, Ludolph A, McGrath M, Manfredi G, Maragakis N, Miller RG, Pullman SL, Rutkove SB, Shaw PJ, Shefner J, Fischbeck KH (2013) Mechanisms, models and biomarkers in amyotrophic lateral sclerosis. Amyotroph Lateral Scler Frontotemporal Degener 14(Suppl 1):19–32. CrossRefGoogle Scholar
  12. 12.
    Salim S (2017) Oxidative stress and the central nervous system. J Pharmacol Exp Ther 360(1):201–205. CrossRefGoogle Scholar
  13. 13.
    Contestabile A (2001) Oxidative stress in neurodegeneration: mechanisms and therapeutic perspectives. Curr Top Med Chem 1(6):553–568CrossRefGoogle Scholar
  14. 14.
    Bond L, Bernhardt K, Madria P, Sorrentino K, Scelsi H, Mitchell CS (2018) A metadata analysis of oxidative stress etiology in preclinical amyotrophic lateral sclerosis: benefits of antioxidant therapy. Front Neurosci 12:10. CrossRefGoogle Scholar
  15. 15.
    Nicolas A, Kenna KP, Renton AE, Ticozzi N, Faghri F, Chia R, Dominov JA, Kenna BJ, Nalls MA, Keagle P, Rivera AM, van Rheenen W, Murphy NA, van Vugt J, Geiger JT, Van der Spek RA, Pliner HA, Shankaracharya, Smith BN, Marangi G, Topp SD, Abramzon Y, Gkazi AS, Eicher JD, Kenna A, Mora G, Calvo A, Mazzini L, Riva N, Mandrioli J, Caponnetto C, Battistini S, Volanti P, La Bella V, Conforti FL, Borghero G, Messina S, Simone IL, Trojsi F, Salvi F, Logullo FO, D'Alfonso S, Corrado L, Capasso M, Ferrucci L, Moreno CAM, Kamalakaran S, Goldstein DB, Gitler AD, Harris T, Myers RM, Phatnani H, Musunuri RL, Evani US, Abhyankar A, Zody MC, Kaye J, Finkbeiner S, Wyman SK, LeNail A, Lima L, Fraenkel E, Svendsen CN, Thompson LM, Van Eyk JE, Berry JD, Miller TM, Kolb SJ, Cudkowicz M, Baxi E, Benatar M, Taylor JP, Rampersaud E, Wu G, Wuu J, Lauria G, Verde F, Fogh I, Tiloca C, Comi GP, Soraru G, Cereda C, Corcia P, Laaksovirta H, Myllykangas L, Jansson L, Valori M, Ealing J, Hamdalla H, Rollinson S, Pickering-Brown S, Orrell RW, Sidle KC, Malaspina A, Hardy J, Singleton AB, Johnson JO, Arepalli S, Sapp PC, McKenna-Yasek D, Polak M, Asress S, Al-Sarraj S, King A, Troakes C, Vance C, de Belleroche J, Baas F, Ten Asbroek A, Munoz-Blanco JL, Hernandez DG, Ding J, Gibbs JR, Scholz SW, Floeter MK, Campbell RH, Landi F, Bowser R, Pulst SM, Ravits JM, MacGowan DJL, Kirby J, Pioro EP, Pamphlett R, Broach J, Gerhard G, Dunckley TL, Brady CB, Kowall NW, Troncoso JC, Le Ber I, Mouzat K, Lumbroso S, Heiman-Patterson TD, Kamel F, Van Den Bosch L, Baloh RH, Strom TM, Meitinger T, Shatunov A, Van Eijk KR, de Carvalho M, Kooyman M, Middelkoop B, Moisse M, McLaughlin RL, Van Es MA, Weber M, Boylan KB, Van Blitterswijk M, Rademakers R, Morrison KE, Basak AN, Mora JS, Drory VE, Shaw PJ, Turner MR, Talbot K, Hardiman O, Williams KL, Fifita JA, Nicholson GA, Blair IP, Rouleau GA, Esteban-Perez J, Garcia-Redondo A, Al-Chalabi A, Rogaeva E, Zinman L, Ostrow LW, Maragakis NJ, Rothstein JD, Simmons Z, Cooper-Knock J, Brice A, Goutman SA, Feldman EL, Gibson SB, Taroni F, Ratti A, Gellera C, Van Damme P, Robberecht W, Fratta P, Sabatelli M, Lunetta C, Ludolph AC, Andersen PM, Weishaupt JH, Camu W, Trojanowski JQ, Van Deerlin VM, Brown RH Jr, van den Berg LH, Veldink JH, Harms MB, Glass JD, Stone DJ, Tienari P, Silani V, Chio A, Shaw CE, Traynor BJ, Landers JE (2018) Genome-wide analyses identify KIF5A as a novel ALS gene. Neuron 97(6):1268–1283.e1266. CrossRefGoogle Scholar
  16. 16.
    Taylor JP, Brown RH Jr, Cleveland DW (2016) Decoding ALS: from genes to mechanism. Nature 539(7628):197–206. CrossRefGoogle Scholar
  17. 17.
    Vinceti M, Filippini T, Violi F, Rothman KJ, Costanzini S, Malagoli C, Wise LA, Odone A, Signorelli C, Iacuzio L, Arcolin E, Mandrioli J, Fini N, Patti F, Lo Fermo S, Pietrini V, Teggi S, Ghermandi G, Scillieri R, Ledda C, Mauceri C, Sciacca S, Fiore M, Ferrante M (2017) Pesticide exposure assessed through agricultural crop proximity and risk of amyotrophic lateral sclerosis. Environ Health 16(1):91. CrossRefGoogle Scholar
  18. 18.
    De Marchi F, Corrado L, Bersano E, Sarnelli MF, Solara V, D'Alfonso S, Cantello R, Mazzini L (2018) Ptosis and bulbar onset: an unusual phenotype of familial ALS? Neurol Sci 39(2):377–378. CrossRefGoogle Scholar
  19. 19.
    Tummala H, Kirwan M, Walne AJ, Hossain U, Jackson N, Pondarre C, Plagnol V, Vulliamy T, Dokal I (2014) ERCC6L2 mutations link a distinct bone-marrow-failure syndrome to DNA repair and mitochondrial function. Am J Hum Genet 94(2):246–256. CrossRefGoogle Scholar
  20. 20.
    Dutta A, Sardiu M, Gogol M, Gilmore J, Zhang D, Florens L, Abmayr SM, Washburn MP, Workman JL (2017) Composition and function of mutant Swi/Snf complexes. Cell Rep 18(9):2124–2134. CrossRefGoogle Scholar
  21. 21.
    Flaus A, Martin DM, Barton GJ, Owen-Hughes T (2006) Identification of multiple distinct Snf2 subfamilies with conserved structural motifs. Nucleic Acids Res 34(10):2887–2905. CrossRefGoogle Scholar
  22. 22.
    Biernacka JM, Chung SJ, Armasu SM, Anderson KS, Lill CM, Bertram L, Ahlskog JE, Brighina L, Frigerio R, Maraganore DM (2016) Genome-wide gene-environment interaction analysis of pesticide exposure and risk of Parkinson’s disease. Parkinsonism Relat Disord 32:25–30. CrossRefGoogle Scholar
  23. 23.
    Theuns J, Verstraeten A, Sleegers K, Wauters E, Gijselinck I, Smolders S, Crosiers D, Corsmit E, Elinck E, Sharma M, Kruger R, Lesage S, Brice A, Chung SJ, Kim MJ, Kim YJ, Ross OA, Wszolek ZK, Rogaeva E, Xi Z, Lang AE, Klein C, Weissbach A, Mellick GD, Silburn PA, Hadjigeorgiou GM, Dardiotis E, Hattori N, Ogaki K, Tan EK, Zhao Y, Aasly J, Valente EM, Petrucci S, Annesi G, Quattrone A, Ferrarese C, Brighina L, Deutschlander A, Puschmann A, Nilsson C, Garraux G, LeDoux MS, Pfeiffer RF, Boczarska-Jedynak M, Opala G, Maraganore DM, Engelborghs S, De Deyn PP, Cras P, Cruts M, Van Broeckhoven C (2014) Global investigation and meta-analysis of the C9orf72 (G4C2)n repeat in Parkinson disease. Neurology 83(21):1906–1913. CrossRefGoogle Scholar
  24. 24.
    Ludolph A, Drory V, Hardiman O, Nakano I, Ravits J, Robberecht W, Shefner J (2015) A revision of the El Escorial criteria - 2015. Amyotroph Lateral Scler Frontotemporal Degener 16(5–6):291–292. CrossRefGoogle Scholar
  25. 25.
    Dardiotis E, Paterakis K, Siokas V, Tsivgoulis G, Dardioti M, Grigoriadis S, Simeonidou C, Komnos A, Kapsalaki E, Fountas K, Hadjigeorgiou GM (2015) Effect of angiotensin-converting enzyme tag single nucleotide polymorphisms on the outcome of patients with traumatic brain injury. Pharmacogenet Genomics 25(10):485–490. CrossRefGoogle Scholar
  26. 26.
    Siokas V, Kardaras D, Aloizou AM, Asproudis I, Boboridis KG, Papageorgiou E, Hadjigeorgiou GM, Tsironi EE, Dardiotis E (2018) BDNF rs6265 (Val66Met) polymorphism as a risk factor for blepharospasm. NeuroMolecular Med 21:68–74. CrossRefGoogle Scholar
  27. 27.
    Siokas V, Kardaras D, Aloizou AM, Asproudis I, Boboridis KG, Papageorgiou E, Spandidos DA, Tsatsakis A, Tsironi EE, Dardiotis E (2019) Lack of association of the rs11655081 ARSG gene with blepharospasm. J Mol Neurosci.
  28. 28.
    Dardiotis E, Paterakis K, Tsivgoulis G, Tsintou M, Hadjigeorgiou GF, Dardioti M, Grigoriadis S, Simeonidou C, Komnos A, Kapsalaki E, Fountas K, Hadjigeorgiou GM (2014) AQP4 tag single nucleotide polymorphisms in patients with traumatic brain injury. J Neurotrauma 31(23):1920–1926. CrossRefGoogle Scholar
  29. 29.
    Skol AD, Scott LJ, Abecasis GR, Boehnke M (2006) Joint analysis is more efficient than replication-based analysis for two-stage genome-wide association studies. Nat Genet 38(2):209–213. CrossRefGoogle Scholar
  30. 30.
    Sole X, Guino E, Valls J, Iniesta R, Moreno V (2006) SNPStats: a web tool for the analysis of association studies. Bioinformatics 22(15):1928–1929. CrossRefGoogle Scholar
  31. 31.
    Dardiotis E, Siokas V, Marogianni C, Aloizou AM, Sokratous M, Paterakis K, Dardioti M, Grigoriadis S, Brotis A, Kapsalaki E, Fountas K, Jagiella J, Hadjigeorgiou GM (2018) AQP4 tag SNPs in patients with intracerebral hemorrhage in Greek and Polish population. Neurosci Lett 696:156–161. CrossRefGoogle Scholar
  32. 32.
    Di Meo S, Reed TT, Venditti P, Victor VM (2016) Role of ROS and RNS sources in physiological and pathological conditions. Oxidative Med Cell Longev 2016:1245049–1245044. Google Scholar
  33. 33.
    Giasson BI, Duda JE, Quinn SM, Zhang B, Trojanowski JQ, Lee VM (2002) Neuronal alpha-synucleinopathy with severe movement disorder in mice expressing A53T human alpha-synuclein. Neuron 34(4):521–533CrossRefGoogle Scholar
  34. 34.
    Giasson BI, Ischiropoulos H, Lee VM, Trojanowski JQ (2002) The relationship between oxidative/nitrative stress and pathological inclusions in Alzheimer’s and Parkinson’s diseases. Free Radic Biol Med 32(12):1264–1275CrossRefGoogle Scholar
  35. 35.
    Zintzaras E (2010) Impact of Hardy-Weinberg equilibrium deviation on allele-based risk effect of genetic association studies and meta-analysis. Eur J Epidemiol 25(8):553–560. CrossRefGoogle Scholar
  36. 36.
    Rasmussen L, Delabio R, Horiguchi L, Mizumoto I, Terazaki CR, Mazzotti D, Bertolucci PH, Pinhel MA, Souza D, Krieger H, Kawamata C, Minett T, Smith MC, Payao SL (2013) Association between interleukin 6 gene haplotype and Alzheimer’s disease: a Brazilian case-control study. J Alzheimers Dis 36(4):733–738. CrossRefGoogle Scholar
  37. 37.
    Mitropoulos K, Merkouri Papadima E, Xiromerisiou G, Balasopoulou A, Charalampidou K, Galani V, Zafeiri KV, Dardiotis E, Ralli S, Deretzi G, John A, Kydonopoulou K, Papadopoulou E, di Pardo A, Akcimen F, Loizedda A, Dobricic V, Novakovic I, Kostic VS, Mizzi C, Peters BA, Basak N, Orru S, Kiskinis E, Cooper DN, Gerou S, Drmanac R, Bartsakoulia M, Tsermpini EE, Hadjigeorgiou GM, Ali BR, Katsila T, Patrinos GP (2017) Genomic variants in the FTO gene are associated with sporadic amyotrophic lateral sclerosis in Greek patients. Hum Genomics 11(1):30. CrossRefGoogle Scholar
  38. 38.
    Pan LS, Deng XB, Wang Z, Leng HL, Zhu XP, Ding D (2016) Lack of association between the Angiogenin (ANG) rs11701 polymorphism and amyotrophic lateral sclerosis risk: a meta-analysis. Neurol Sci 37(5):655–662. CrossRefGoogle Scholar
  39. 39.
    Pehar M, Beeson G, Beeson CC, Johnson JA, Vargas MR (2014) Mitochondria-targeted catalase reverts the neurotoxicity of hSOD1G(9)(3)A astrocytes without extending the survival of ALS-linked mutant hSOD1 mice. PLoS One 9(7):e103438. CrossRefGoogle Scholar
  40. 40.
    Rosen DR, Siddique T, Patterson D, Figlewicz DA, Sapp P, Hentati A, Donaldson D, Goto J, O'Regan JP, Deng HX, Rahmani Z, Krizus A, McKenna-Yasek D, Cayabyab A, Gaston SM, Berger R, Tanzi RE, Halperin JJ, Herzfeldt B, van den Bergh R, Hung WY, Bird T, Deng G, Mulder DW, Smyth C, Laing NG, Soriano E, Pericak–Vance MA, Haines J, Rouleau GA, Gusella JS, Horvitz HR, Brown RH (1993) Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature 362(6415):59–62. CrossRefGoogle Scholar
  41. 41.
    Carri MT, Valle C, Bozzo F, Cozzolino M (2015) Oxidative stress and mitochondrial damage: importance in non-SOD1 ALS. Front Cell Neurosci 9:41. Google Scholar
  42. 42.
    Rabdano SO, Izmailov SA, Luzik DA, Groves A, Podkorytov IS, Skrynnikov NR (2017) Onset of disorder and protein aggregation due to oxidation-induced intermolecular disulfide bonds: case study of RRM2 domain from TDP-43. Sci Rep 7(1):11161. CrossRefGoogle Scholar
  43. 43.
    Stefanidis I, Vainas A, Dardiotis E, Giannaki CD, Gourli P, Papadopoulou D, Vakianis P, Patsidis E, Eleftheriadis T, Liakopoulos V, Pournaras S, Sakkas GK, Zintzaras E, Hadjigeorgiou GM (2013) Restless legs syndrome in hemodialysis patients: an epidemiologic survey in Greece. Sleep Med 14(12):1381–1386. CrossRefGoogle Scholar
  44. 44.
    Paganoni S, Cudkowicz M, Berry JD (2014) Outcome measures in amyotrophic lateral sclerosis clinical trials. Clin Investig 4(7):605–618CrossRefGoogle Scholar
  45. 45.
    Siokas V, Aloizou AM, Tsouris Z, Michalopoulou A, Mentis AA, Dardiotis E (2018) Risk factor genes in patients with dystonia: a comprehensive review. Tremor Other Hyperkinet Mov (N Y) 8:559. Google Scholar
  46. 46.
    Dardiotis E, Koutsou P, Zamba-Papanicolaou E, Vonta I, Hadjivassiliou M, Hadjigeorgiou G, Cariolou M, Christodoulou K, Kyriakides T (2009) Complement C1Q polymorphisms modulate onset in familial amyloidotic polyneuropathy TTR Val30Met. J Neurol Sci 284(1–2):158–162. CrossRefGoogle Scholar
  47. 47.
    Anastasiou CA, Yannakoulia M, Kosmidis MH, Dardiotis E, Hadjigeorgiou GM, Sakka P, Arampatzi X, Bougea A, Labropoulos I, Scarmeas N (2017) Mediterranean diet and cognitive health: initial results from the Hellenic longitudinal investigation of ageing and diet. PLoS One 12(8):e0182048. CrossRefGoogle Scholar
  48. 48.
    Dardiotis E, Aloizou AM, Siokas V, Tsouris Z, Rikos D, Marogianni C, Aschner M, Kovatsi L, Bogdanos DP, Tsatsakis A (2019) Paraoxonase-1 genetic polymorphisms in organophosphate metabolism. Toxicology 411:24–31. CrossRefGoogle Scholar
  49. 49.
    Hadjigeorgiou GM, Malizos K, Dardiotis E, Aggelakis K, Dardioti M, Zibis A, Dimitroulias A, Scarmeas N, Tsezou A, Karantanas A (2007) Paraoxonase 1 gene polymorphisms in patients with osteonecrosis of the femoral head with and without cerebral white matter lesions. J Orthop Res 25(8):1087–1093. CrossRefGoogle Scholar
  50. 50.
    Castelnovo V, Caminiti SP, Riva N, Magnani G, Silani V, Perani D (2018) Heterogeneous brain FDG-PET metabolic patterns in patients with C9orf72 mutation. Neurol Sci.

Copyright information

© Fondazione Società Italiana di Neurologia 2019

Authors and Affiliations

  • Efthimios Dardiotis
    • 1
    Email author
  • Emmanouil Karampinis
    • 1
  • Vasileios Siokas
    • 1
  • Athina-Maria Aloizou
    • 1
  • Dimitrios Rikos
    • 1
  • Styliani Ralli
    • 1
  • Dimitra Papadimitriou
    • 2
  • Dimitrios P. Bogdanos
    • 3
    • 4
  • Georgios M. Hadjigeorgiou
    • 5
  1. 1.Department of Neurology, University Hospital of Larissa, Laboratory of Neurogenetics, Faculty of MedicineUniversity of ThessalyLarissaGreece
  2. 2.Neurological DepartmentHenry Dunant Hospital CenterAthensGreece
  3. 3.Department of Rheumatology and Clinical Immunology, University General Hospital of Larissa, Faculty of Medicine, School of Health SciencesUniversity of ThessalyLarissaGreece
  4. 4.Cellular Immunotherapy & Molecular Immunodiagnostics, Biomedical SectionCentre for Research and Technology-Hellas (CERTH)- Institute for Research and Technology-Thessaly (IRETETH)LarissaGreece
  5. 5.Department of Neurology, Medical SchoolUniversity of CyprusNicosiaCyprus

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