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Profiling of microRNAs modulating cytomegalovirus infection in astrocytoma patients

  • Ravindra Pramod Deshpande
  • Manas Panigrahi
  • Chandrasekhar Y.B.V.K.
  • Phanithi Prakash Babu
Original Article

Abstract

Astrocytoma is recognized as the most common neoplasm of the brain with aggressive progression. The therapeutic regime for glioblastoma, the most aggressive astrocytoma, often consists of aggressive chemo and radiotherapy. The present holistic approaches, however, have failed to influence the quality life of patients. Therefore, it is necessary to understand the underlying mechanisms of its progression for updated therapeutic evaluation. Human cytomegalovirus (HCMV) is reported to be associated with glioblastoma progression. The hypothesis still remains controversial due to the lack of concrete evidences. Here, we report the profile of miRNAs encoded by human host and the cytomegalovirus (CMV) involved in modulation of CMV infection in surgically resected human astrocytoma tissue samples of various malignancy grades (n = 24). Total RNA from the control brain and tumor tissues was extracted by TriZol reagent. The expression levels of the mature form of miRNA were detected by real-time PCR. Primarily, we found the upregulation of miR-210-3p, miR-155-5p, miR-UL-112-3p, miR-183-5p, and miR-223-5p in high-grade astrocytic tumors as compared with low-grade tumor tissues. miR-214-3p is significantly expressed in control brain tissues and its expression decreased with astrocytoma grade progression. This miRNA was reported to be associated with antiviral proprieties. Among CMV-encoded miRNA, miR-UL-112-3p was significantly upregulated in glioblastoma tissue samples and may be involved in providing immune escape to the virus as well as involved in modulating the immune microenvironment of glioblastoma. Taken together, we conclude the possible involvement of miRNAs in modulating the CMV dependent astrocytoma progression.

Keywords

Glioblastoma Astrocytoma CMV miRNA RT PCR 

Notes

Author contributions

Conception and design: Ravindra Pramod Deshpande

Provision of study material: Manas Panigrahi, Chandrasekhar Y.B.V.K.

Collection and assembly of data: Ravindra Pramod Deshpande

Analysis and interpretation of data: Ravindra Pramod Deshpande, Phanithi Prakash Babu

Manuscript writing: Ravindra Pramod Deshpande, Phanithi Prakash Babu

Final approval: All authors

Funding

Authors thank financial assistance from Department of Science and technology (DST-India) (Grant nos. SB/EMEQ-257/2013, SR/CSRI/196/2016), Department of Biotechnology (DBT-India) (Grant nos. BT/PR18168/MED/29/1064/2016, BT/PR13111/MED/29/149/2009), Indian Council of Medical research (ICMR-India) (30/2/2010-01710/SIC/PI/N/162) for lab funding. RDP is thankful to Department of Biotechnology (DBT-India) (Award no: DBT JRF/2011-12/95) for student fellowship.

Compliance with ethical standards

Ethical permissions

The authors acknowledge KIMS Foundation Research Center (KFRC) and Institutional Ethical Committee (IEC), University of Hyderabad for granting the ethical permissions. Performed studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflict of interest

All authors declare that there are no conflicts of interest.

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© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biotechnology and Bioinformatics, School of Life SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Krishna Institute of Medical SciencesSecunderabadIndia

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