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Anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. and propolis extracts

  • Yun Kyong Lim
  • So Young Yoo
  • Young Yoon Jang
  • Byoung Cheon Lee
  • Dae Sung Lee
  • Joong-Ki KookEmail author
Article
  • 47 Downloads

Abstract

The purpose of this study was to determine the anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. (mangosteen) and propolis extracts. Immortalized human gingival fibroblasts (hTERT-hNOF) cells were treated with Porphyromonas gingivalis KCOM 2804 lipopolysaccharide followed by treatment with mangosteen and propolis extract alone or in combination. Expression levels of inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay. Effect of mangosteen and/or propolis extracts on mineralization of MG-63 cells was evaluated by alkaline phosphatase activity and alizarin red S staining. Group mangosteen extract complex 1:34 (1 µg/ml mangosteen extract and 34 µg/ml propolis extract) significantly reduced expression levels of IL-6, IL-8, and PGE2. It had higher than other groups in vitro bone formation effect on MG63 cells. These results suggest that mangosteen and propolis extract complex could be used in the prevention and treatment of periodontal disease.

Keywords

Garcinia mangostana L. Propolis Anti-inflammatory In vitro bone formation 

Notes

Acknowledgements

This research was supported by Med Lab Co., Ltd. (2016-0110).

Compliance with ethical standards

Conflict of interest

Dae Sung Lee is a co-register on patent (No. 1018793980000, Korea) entitled “Pharmaceutical composition for enhancing osteogenesis comprising garcinia mangostana extract and propolis extract” and on patent (Nos. US 10,022,412 B2 and JP 6387467 B2) entitled “Composition for preventing or alleviating periodontal diseases, containing, as active ingredients, mangosteen extract or α- or γ-mangosteen. The authors declare that they have no conflict of interest.

Supplementary material

10068_2019_697_MOESM1_ESM.pptx (3.7 mb)
Supplementary Fig. S1. Flow chart for preparing the mangosteen extract used in this study. Supplementary Fig. S2. Certificate of the analysis of the composition of mangosteen extract used in this study. Supplementary Fig. S3. Schematic procedure for preparing the propolis extract used in this study. Supplementary Fig. S4. Certificate of analysis of the composition of the propolis extract used in this study. (PPTX 3771 kb)

References

  1. Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Ozkut MM, Inan S. Systemic propolis stimulates new bone formation at the expanded suture: a histomorphometric study. Angle Orthod. 83: 286-291 (2013)CrossRefPubMedGoogle Scholar
  2. Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F, Ialenti A. Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia 73: 53-63 (2002)CrossRefGoogle Scholar
  3. Chen LG, Yang LL, Wang CC. Anti-inflammatory activity of mangostins from Garcinia mangostana. Food Chem. Toxicol. 46: 688-693 (2008)CrossRefPubMedGoogle Scholar
  4. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents. 26: 343-356 (2005). Erratum in: Int. J. Antimicrob. Agents. 27: 181 (2006)Google Scholar
  5. Feng J, Shi Z, Ye Z. Effects of metabolites of the lignans enterolactone and enterodiol on osteoblastic differentiation of MG-63 cells. Biol. Pharm. Bull. 31: 1067-1070 (2008)CrossRefPubMedGoogle Scholar
  6. Fujita Y, Ito H, Sekino S, Numabe Y. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology 100: 215-221 (2012)CrossRefPubMedGoogle Scholar
  7. Gutierrez-Orozco F, Chitchumroonchokchai C, Lesinski GB, Suksamrarn S, Failla ML. α-Mangostin: anti-inflammatory activity and metabolism by human cells. J. Agric. Food Chem. 61: 3891-3900 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  8. Gutierrez-Orozco F, Failla ML. Biological activities and bioavailability of mangosteen xanthones: a critical review of the current evidence. Nutrient 5: 3163-3183 (2013)CrossRefGoogle Scholar
  9. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 13: 572-584 (2002)CrossRefGoogle Scholar
  10. Idrus E, Bramma K. Mangosteen extract inhibits LPS-induced bone resorption by controlling osteoclast. J. Int. Dent. Med. Res. 9: 362-367 (2016)Google Scholar
  11. Johnson JJ, Petiwala SM, Syed DN, Rasmussen JT, Adhami VM, Siddiqui IA, Kohl AM, Mukhtar H. α-Mangostin, a xanthone from mangosteen fruit, promotes cell cycle arrest in prostate cancer and decreases xenograft tumor growth. Carcinogenesis 33: 413-419 (2012)CrossRefPubMedGoogle Scholar
  12. Kern B, Shen J, Starbuck M, Karsenty G. Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J. Biol. Chem. 276: 7101-7107 (2001)CrossRefPubMedGoogle Scholar
  13. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89: 755-764 (1997)CrossRefPubMedGoogle Scholar
  14. Liu J, Tang X, Li C, Pan C, Li Q, Geng F, Pan Y. Porphyromonas gingivalis promotes the cell cycle and inflammatory cytokine production in periodontal ligament fibroblasts. Arch. Oral Biol. 60: 1153-1161 (2015)CrossRefPubMedGoogle Scholar
  15. Michaluart P, Masferrer JL, Carothers AM. Inhibition effects of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cells and in a rat model of inflammation. Cancer Res. 59: 2347-2352 (1999)PubMedGoogle Scholar
  16. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, de Crombrugghe B. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108: 17-29 (2002)CrossRefGoogle Scholar
  17. Nguyen PT, Marquis RE. Antimicrobial actions of α-mangostin against oral streptococci. Can. J. Microbiol. 57: 217-225 (2011)CrossRefPubMedGoogle Scholar
  18. Occhiuto F, Pasquale RD, Guglielmo G, Palumbo DR, Zangla G, Samperi S, Renzo A, Circosta C. Effects of phytoestrogenic isoflavones from red clover (Trifolium pratense L.) on experimental osteoporosis. Phytother. Res. 21: 130-134 (2007)CrossRefPubMedGoogle Scholar
  19. Okada N, Kobayashi M, Mugikura K, Okamatsu Y, Hanazawa S, Kitano S, Hasegawa K. Interleukin-6 production in human fibroblasts derived from periodontal tissues is differentially regulated by cytokines and a glucocorticoid. J. Periodontal Res. 32: 559-569 (1997)CrossRefPubMedGoogle Scholar
  20. Pai MR, Acharya LD, Udupa N. Evaluation of antiplaque activity of Azadirachta indica leaf extract gel—a 6-week clinical study. J. Ethnopharmacol. 90: 99-103 (2004)CrossRefPubMedGoogle Scholar
  21. Palombo EA. Traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases. Evid. Based Complement. Alternat. Med. 2011: 1-15 (2009)CrossRefGoogle Scholar
  22. Puchtler H, Meloan SN, Terry MS. On the history and mechanism of alizarin and alizarin red S stains for calcium. J. Histochem. Cytochem. 17: 110-124 (1969)CrossRefPubMedGoogle Scholar
  23. Ramos AFN, Miranda JL. Propolis: a review of its anti-inflammatory and healing actions. J. Venom. Anim. Toxins Incl. Trop. Dis. 13: 697-710 (2007)CrossRefGoogle Scholar
  24. Raso GM, Meli R, Carlo GD, Pacilio M, Carlo RD. Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A. 1. Life Sci. 68: 921-931 (2001)CrossRefPubMedGoogle Scholar
  25. Reinholz GG, Getz B, Pederson L, Sanders ES, Subramaniam M, Ingle JN, Spelsberg TC. Bisphosphonates directly regulate cell proliferation, differentiation, and gene expression in human osteoblasts. Cancer Res. 60: 6001-6007 (2000)PubMedGoogle Scholar
  26. Sánchez GA, Miozza VA, Delgado A, Busch L. Salivary IL-1β and PGE2 as biomarkers of periodontal status, before and after periodontal treatment. J. Clin. Periodontol. 40: 1112-1117 (2013)CrossRefPubMedGoogle Scholar
  27. Socransky SS, Smith C, Haffajee AD. Subgingival microbial profiles in refractory periodontal disease. J. Clin. Periodontol. 29: 260-268 (2002)CrossRefPubMedGoogle Scholar
  28. Suzuki N, Yoneda M, Hirofuji T. Mixed red-complex bacterial infection in periodontitis. Int. J. Dent. 2013: 587279 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  29. Tjahjani S, Widowati W, Khiong K, Suhendra A, Tjokropranoto R. Antioxidant properties of Garcinia mangostana L. (Mangosteen) Rind. Procedia Chem. 13: 198-203 (2014)CrossRefGoogle Scholar
  30. Tredwin C, Scully C, Bagan-Sebastian J. Drug-induced disorders of teeth. J. Dental Res. 84: 596-602 (2005)CrossRefGoogle Scholar
  31. van den Dolder J, Bancroft GN, Sikavitsas VI, Spauwen PH, Jansen JA, Mikos AG. Flow perfusion culture of marrow stromal osteoblasts in titanium fiber mesh. J. Biomed. Mater. Res. A. 64: 235-241 (2003)CrossRefPubMedGoogle Scholar
  32. Yiemwattana I, Chaisomboon N, Jamdee K. Antibacterial and anti-inflammatory potential of Morus alba stem extract. Open Dent. J. 12: 265-274 (2018)CrossRefPubMedPubMedCentralGoogle Scholar
  33. Yiemwattana I, Ruchadaporn, K. Alpha-mangostin suppresses IL-6 and IL-8 expression in LPS-stimulated human gingival fibroblasts. Odontology 103: 348-355 (2015)CrossRefPubMedGoogle Scholar
  34. Zhao J, Tan L, Lin L, Wang H, Pan C, Pan Y. Local and peripheral cytokines profiling on Porphyromonas gingivalis induced rat periodontitis model. Int. J. Clin. Exp. Med. 9: 5996-6004 (2016)Google Scholar

Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  1. 1.Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of DentistryChosun UniversityGwangjuRepublic of Korea
  2. 2.Medi Bio Lab Co., Ltd.SeoulRepublic of Korea
  3. 3.Jong Kun Dang PharmacySeoulRepublic of Korea

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