Versatile biotechnological applications of amylosucrase, a novel glucosyltransferase
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Amylosucrase (AS; EC 22.214.171.124) is an enzyme that has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize α-1,4-glucans, like amylose, from sucrose as a sole substrate, but importantly, it can also utilize various other molecules as acceptors. In addition, AS produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. Furthermore, AS produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by AS forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences. This review not only compares the gene and enzyme characteristics of microbial AS, studied to date, but also focuses on the applications of AS in the biotechnology and food industries.
KeywordsAmylosucrase Transglycosylation Enzymatically modified starch Turanose Amylose
This research was supported by the National Research Foundation of Korea (NRF) Grant (No. 2018R1C1B6001459) funded by the Korea government (MSIT).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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