Anti-dermatitic effect of fermented ginseng extract including rich compound K through inhibiting activation of macrophage
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The purpose of this study is to investigate the effect of fermented ginseng extract by Lactobacillus brevis (FGE) on lipopolysaccharide (LPS)-activated macrophages and 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated dermatitis in mice. FGE showed better anti-inflammatory activities than ginseng extract on the formation of nitric monooxide, IL-6, TNF-α, and IL-10 within non-cytotoxicity range in LPS-activated RAW264.7 cells. In addition, FGE reduced the expression of cyclooxygenase-2 and inducible nitric oxide synthase through inhibiting nuclear translocation of NF-κB. Consistent with in vitro experiments, FGE dose-dependently suppressed ear edema, and formation of TNF-α and IL-6, and it (50 mg/mL) significantly enhanced IL-10 level in ear tissues of TPA-treated mice. In conclusions, FGE has anti-dermatitic activity through inhibiting the activation of macrophages. Such effects of FGE are associated with suppressing nuclear translocation of NF-κB. Therefore, the features of FGE may provide the information for its application for therapy and prevention of dermatitis.
KeywordsCompound K Contact dermatitis Fermented ginseng Lactobacillus brevis Macrophage
Extract of fermented ginseng fine root
Extract of ginseng fine root
Inducible nitric oxide synthase
Toll-like receptor 4
Tumor necrosis factor-α
This work was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant Number 2017R1D1A3B03027867).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights
All institutional and national guidelines for the care and use of laboratory animals were followed.
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