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High-performance liquid chromatography and gas chromatography to set the analysis method of stearoyl lactylate, a food emulsifier

  • Juhee Park
  • Hyondeog Kim
  • Seungran Hong
  • Hee-Jae Suh
  • Chan LeeEmail author
Article
  • 5 Downloads

Abstract

Stearoyl lactylates (SLs, E481/E482) and their sodium and calcium salts are emulsifiers commonly used as food additives. This study was performed to set the analytical method of SLs in Korea using high-performance liquid chromatography (HPLC) and gas chromatography (GC). A new HPLC method using C18 column with water and methanol as the eluents, and a new GC method using DB-1 column with He as the carrier gas, were developed and were compared with previously reported analytical methods. The new HPLC and GC methods indicated a coefficient of determination (r2) of 0.999 in the calibration curves. The detection and quantification limits are 0.26 and 0.78 μg/kg for the HPLC method, respectively, and 16.54 and 50.12 μg/kg for the GC method, respectively. The new HPLC and GC methods indicated precision of 0–2.0%, and accuracy of 92.7–108.5%. Therefore, they can be applied in numerous food samples to determine the level of SLs.

Keywords

Stearoyl lactylates High-performance liquid chromatography (HPLC) analysis Gas chromatography-flame ionization detector analysis Sodium stearoyl lactylate Calcium stearoyl lactylate 

Notes

Acknowledgements

This research was supported by a Grant (17162MFDS021) from the Ministry of Food and Drug Safety in 2017. This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2017.

References

  1. AOAC Peer Verified Methods Advisory Committee. AOAC peer verified methods program. Manual on policies and procedures. AOAC International, Gaithersburg, MD, USA pp. 1–35 (1998)Google Scholar
  2. Brüschweiler H, Hautfenne A. Determination of the ester-emulsifiers components content after hydrolysis and silylation by gas chromatography. Pure Appl. Chem. 62: 781–793 (1990)CrossRefGoogle Scholar
  3. CODEX Alimentarius. GSFA online, food additive group details. Available from: http://www.fao.org/gsfaonline/groups/details.html?id=95 Accessed Dec. 20, 2018.
  4. Cucu T, Shrestha K, De Meulenaer B. Development of a quantitative GC-FID method for the determination of stearoyl-lactylates (E481/482) in foods. Food Addict. Contam. Part A 31: 1929–1938 (2014)CrossRefGoogle Scholar
  5. De Stefanis VA, Ponte JG, Jr. Chung FH, Ruzza NA. Binding of crumb softeners and dough strengtheners during breadmaking. AACC Int. Cereal Chem. 54: 13–24 (1977)Google Scholar
  6. EFSA ANS Panel (EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS)). Scientific opinion on the re-evaluation of sodium stearoyl-2-lactylate (E 481) and calcium stearoyl-2-lactylate (E 482) as food additives. EFSA J. 11: 3144 (2013)Google Scholar
  7. European Commission. Commission regulation (EU) no 257/2010 of 25 March 2010 setting up a programme for the re-evaluation of approved food additives in accordance with regulation (EC) no. 1333/2008 of the European Parliament and of the Council on Food Additives. Official Journal of the European Union, L 80/19 (2010)Google Scholar
  8. European Commission (EC). Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed. Supersedes Document No. SANTE/11945/2015 (2017)Google Scholar
  9. FDA (Food and Drug Administration). Guidance for industry: Q2B validation of analytical procedures: methodology. Rockville, MD, USA (1996)Google Scholar
  10. FSA (Food Standards Agency). Development of methods for the determination of emulsifiers and polyphosphates in foods. FSA Project (2004)Google Scholar
  11. Gómez M, Del Real S, Rosell CM, Ronda F, Blanco CA, Caballero PA. Functionality of different emulsifiers on the performance of breadmaking and wheat bread quality. Eur. Food Res. Technol. 219(2): 145–150 (2004)CrossRefGoogle Scholar
  12. Gonzalez AG, Herrador MA. A practical guide to analytical method validation, including measurement uncertainty and accuracy profiles. Trends Analyt. Chem. 26: 227–238 (2007)CrossRefGoogle Scholar
  13. Ishidate Jr M, Sofuni T, Yoshikawa K, Hayashi M, Nohmi T, Sawada M, Matsuoka A. Primary mutagenicity screening of food additive currently used in Japan. Food Chem. Toxicol. 22: 623–636 (1984)CrossRefGoogle Scholar
  14. JECFA. Toxicological evaluation of some food additives including anticaking agents, antimicrobials, antioxidants, emulsifiers and thickening agents 539. Stearoyl Lactylic Acid, Calcium and Sodium Salts. Series 5. Wld Hlth Org. (1974)Google Scholar
  15. Lamb J, Hentz K, Schmitt D, Tran N, Jonker D, Junker K. A one-year oral toxicity study of sodium stearoyl lactylate (SSL) in rats. Food Chem. Toxicol. 48: 2663–2669 (2010)CrossRefGoogle Scholar
  16. Loco JV, Elskens M, Croux C, Beernaert H. Linearity of calibration curves: use and misuse of the correlation coefficient. Accred. Qual. Assur. 7: 281–285 (2002)CrossRefGoogle Scholar
  17. Mikawa T, Kubota H, Ozeki Y, Yoshida M, Nakanishi T, Sato K, Akiyama H. Determination of sodium stearoyl lactylates in foods using HPLC after derivatization with 2-nitrophenyl hydrazine. Jpn. J. Food Chem. Saf. 19: 178–184 (2012)Google Scholar
  18. National Health and Family Planning Commission (NHFPC). Chinese Standards for Food Additives – GB2760-2014. Available online: http://www.jlfsstd.net/db/files/Std4_7673135842462310.pdf (2018).
  19. Shabir GA, Lough WJ, Arain SA, Bradshaw TK. Evaluation and application of best practice in analytical method validation. J. Liq. Chromatogr. Relat. Technol. 30: 311–333 (2007)CrossRefGoogle Scholar
  20. Sudraud G, Coustard JM, Retho C. Analytical and structural study of some food emulsifiers by high-performance liquid chromatography and off-line mass spectrometry. J Chromatogr. A. 204: 397–406 (1981)CrossRefGoogle Scholar
  21. Suman M, Silva G, Catellani D, Bersellini U, Caffarra V, Careri M. Determination of food emulsifiers in commercial additives and food products by liquid chromatography/atmospheric-pressure chemical ionisation mass spectrometry. J. Chromatogr. A. 1216: 3758–3766 (2009)CrossRefGoogle Scholar
  22. Taverniers I, Loose M, Bockstaele EV. Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. Trends Analyt. Chem. 23: 535–552 (2004)Google Scholar
  23. The Japan Food Chemical Research Foundation (JFCRF). Standards for use of food additives. Standards for use, according to use categories. Available online: https://www.ffcr.or.jp/en/upload/90a214f8b8b1b90ec5acff9db0a39c194d8df7ae.pdf (2018)
  24. Thewlis BH. The fate of stearoyl lactylates when used in breadmaking. J. Sci. Food Agric. 32: 125–128 (1981)CrossRefGoogle Scholar
  25. Wheeler EL. Quantitative determination of sodium stearoyl-2-lactylate in soy-fortified wheat-flour blends. AACC Int. Cereal Chem. 56: 236–239 (1979)Google Scholar
  26. Whitehurst RJ. Emulsifiers in food technology. 1st ed. Blackwell Publishing Ltd, Northampton, U.K. pp. 206–225 (2004)CrossRefGoogle Scholar
  27. WHO Expert Committee on Specifications for Pharmaceutical Preparations, & World Health Organization. WHO Expert Committee on Specifications for Pharmaceutical Preparations (1993)Google Scholar
  28. WHO Food Additives Series, No. 1. Fifteenth report of the Joint FAO/WHO Expert Committee on Food Additives, World Health Organization (1972)Google Scholar
  29. Yukawa M, Hanada J. Determination of calcium stearoyl lactate in bread. Yukagaku 31: 958–959 (1982)Google Scholar

Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  • Juhee Park
    • 1
  • Hyondeog Kim
    • 1
  • Seungran Hong
    • 1
  • Hee-Jae Suh
    • 2
  • Chan Lee
    • 1
    Email author
  1. 1.Advanced Food Safety Research Group, BrainKorea21 Plus, Department of Food Science and TechnologyChung-Ang UniversityAnseongRepublic of Korea
  2. 2.Department of Food ScienceSun Moon UniversityAsan-SiRepublic of Korea

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