Headspace volatiles influenced by infusion matrix and their release persistence: a case study of oolong tea

  • Jie Lin
  • Yuanxu Shi
  • Chunwang Dong
  • Xiaochang WangEmail author


The perceived aroma of oolong tea is primarily and directly affected by its infusion matrix, and the release persistence of headspace volatiles can better illustrate the persistent aroma. Headspace solid phase microextraction coupled with gas chromatography–mass spectrometry was performed to analyze the headspace constituents of oolong tea. The presence of the infusion matrix seemed to prevent the headspace release of certain odorants. The release of indole, nerolidol, and α-farnesene was also remarkably enhanced or depressed (2.70, 1.56, and 0.69-fold in tea infusion versus in dry leaves). Moreover, the amount of volatile species gradually decreased with increased water ratio. Eight odorants were determined to be stable and persistent during continuous infusion, whereas six were determined to be less persistent (gradually decreased or stopped releasing). The volatile dilution test further confirmed the persistent release of nerolidol.


Volatiles Oolong tea Infusion matrix Aroma persistence Headspace solid phase microextraction (HS-SPME) 



This research was supported by National Natural Science Foundation of China under Grant No. 31800582, and Zhejiang Provincial Natural Science Foundation of China under Grant No. LQ14C160003.

Supplementary material

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Supplementary material 1 (DOCX 27 kb)
10068_2019_587_MOESM2_ESM.docx (26 kb)
Supplementary material 2 (DOCX 26 kb)


  1. Baldermann S, Yang Z, Katsuno T, Tu VA, Mase N, Nakamura Y, Watanabe N. Discrimination of green, oolong, and black teas by GC–MS analysis of characteristic volatile flavor compounds. Am. J. Anal. Chem. 05: 620–632 (2014)CrossRefGoogle Scholar
  2. Caprioli G, Cortese M, Cristalli G, Maggi, F, Odello L, Ricciutelli M, Sagratini G, Sirocchi V, Tomassoni G, Vittori S. Optimization of espresso machine parameters through the analysis of coffee odorants by HS-SPME–GC/MS. Food Chem. 135: 1127–1133 (2012)CrossRefGoogle Scholar
  3. Chen YJ, Kuo PC, Yang ML, Li FY, Tzen JTC. Effects of baking and aging on the changes of phenolic and volatile compounds in the preparation of old Tieguanyin oolong teas. Food Res. Int. 53: 732–743 (2013)CrossRefGoogle Scholar
  4. Cheng Y, Huynh-Ba T, Blank I, Robert F. Temporal changes in aroma release of Longjing tea infusion: interaction of volatile and nonvolatile tea components and formation of 2-butyl-2-octenal upon aging. J. Agric. Food Chem. 56: 2160–2169 (2008)CrossRefGoogle Scholar
  5. Dufour C, Bayonove CL. Interactions between wine polyphenols and aroma substances. An insight at the molecular level. J. Agric. Food Chem. 47: 678–684 (1999)CrossRefGoogle Scholar
  6. Elmore JS, Erbahadir A, Mottram DS. Comparison of dynamic headspace concentration on Tenax with solid phase microextraction for the analysis of aroma volatiles. J. Agric. Food Chem. 45: 2638–2641 (1997)CrossRefGoogle Scholar
  7. Fanaro GB, Duarte RC, Santillo AG, Pinto e Silva MEM, Purgatto E, Villavicencio ALCH. Evaluation of γ-radiation on oolong tea odor volatiles. Radiat. Phys. Chem. 81: 1152–1156 (2012)CrossRefGoogle Scholar
  8. Ho CT, Zheng X, Li S. Tea aroma formation. Food Sci. Hum. Wellness 4: 9–27 (2015)CrossRefGoogle Scholar
  9. Ito Y, Sugimoto A, Kakuda T, Kubota K. Identification of potent odorants in Chinese jasmine green tea scented with flowers of Jasminum sambac. J. Agric. Food Chem. 50: 4878–4884 (2002)CrossRefGoogle Scholar
  10. Jelen HH, Wlazly K, Wasowicz E, Kaminski E. Solid-phase microextraction for the analysis of some alcohols and esters in beer: comparison with static headspace method. J. Agric. Food Chem. 46: 1469–1473 (1998)CrossRefGoogle Scholar
  11. Katsuno T, Kasuga H, Kusano Y, Yaguchi Y, Tomomura M, Cui J, Yang Z, Baldermann S, Nakamura Y, Ohnishi T, Mase N, Watanabe N. Characterisation of odorant compounds and their biochemical formation in green tea with a low temperature storage process. Food Chem. 148: 388–395 (2014)CrossRefGoogle Scholar
  12. Kausar T, Akram K, Kwon JH. Comparative effects of irradiation, fumigation, and storage on the free amino acids and sugar contents of green, black and oolong teas. Radiat. Phys. Chem. 86: 96–101 (2013)CrossRefGoogle Scholar
  13. Kumazawa K, Masuda H. Identification of potent odorants in different green tea varieties using flavor dilution technique. J. Agric. Food Chem. 50: 5660–5663 (2002)CrossRefGoogle Scholar
  14. Lin J, Zhang P, Pan Z, Xu H, Luo Y, Wang X. Discrimination of oolong tea (Camellia sinensis) varieties based on feature extraction and selection from aromatic profiles analysed by HS-SPME/GC–MS. Food Chem. 141: 259–265 (2013)CrossRefGoogle Scholar
  15. Ma C, Qu Y, Zhang Y, Qiu B, Wang Y, Chen X. Determination of nerolidol in teas using headspace solid phase microextraction–gas chromatography. Food Chem. 152: 285–290 (2014)CrossRefGoogle Scholar
  16. Macleod AJ, Panchasara SD. Volatile aroma components, particularly glucosinolate products, of cooked edible mushroom (Agaricus bisporus) and cooked dried mushroom. Phytochemistry. 22: 705–709 (1983)CrossRefGoogle Scholar
  17. Macleod AJ, Pieris NM. Volatile aroma constituents of Sri Lankan ginger. Phytochemistry. 23: 353–359 (1984)CrossRefGoogle Scholar
  18. Mei Y, Wang Z. Investigation report on national production and marketing of Oolong Tea in 2016. Guangdong Tea Industry 1–8 (2017) (in Chinese)Google Scholar
  19. Ogawa K, Moon JH, Guo W, Yagi A, Watanabe N, Sakata K. A study on tea aroma formation mechanism: alcoholic aroma precursor amounts and glycosidase activity in parts of the tea plant. Z. Naturforsch. C Biosci. 50: 493–498 (1995)CrossRefGoogle Scholar
  20. Qin Z, Pang X, Chen D, Cheng H, Hu X, Wu J. Evaluation of Chinese tea by the electronic nose and gas chromatography–mass spectrometry: correlation with sensory properties and classification according to grade level. Food Res. Int. 53: 864–874 (2013)CrossRefGoogle Scholar
  21. Riu-Aumatell M, Castellari M, Lopez-Tamames E, Galassi S, Buxaderas S. Characterisation of volatile compounds of fruit juices and nectars by HS/SPME and GC/MS. Food Chem. 87: 627–637 (2004)CrossRefGoogle Scholar
  22. Robinson AL, Ebeler SE, Heymann H, Boss PK, Solomon PS, Trengove RD. Interactions between wine volatile compounds and grape and wine matrix components influence aroma compound headspace partitioning. J. Agric. Food Chem. 57: 10313–10322 (2009)CrossRefGoogle Scholar
  23. Rodríguez RM, Pando RB, Suárez BV. Production and characterization of aroma compounds from apple pomace by solid-state fermentation with selected yeasts. LWT Food Sci. Technol. 64: 1342–1353 (2015)CrossRefGoogle Scholar
  24. Romeo V, Ziino M, Giuffrida D, Condurso C, Verzera A. Flavour profile of capers (Capparis spinosa L.) from the Eolian Archipelago by HS-SPME/GC–MS. Food Chem. 101: 1272–1278 (2007)CrossRefGoogle Scholar
  25. Schuh C, Schieberle P. Characterization of the key aroma compounds in the beverage prepared from Darjeeling black tea:  quantitative differences between tea leaves and infusion. J. Agric. Food Chem. 54: 916–924 (2006)CrossRefGoogle Scholar
  26. Shimoda M, Shigematsu H, Shiratsuchi H, Osajima Y. Comparison of the odor concentrates by SDE and adsorptive column method from green tea infusion. J. Agric. Food Chem. 43: 1616–1620 (1995)CrossRefGoogle Scholar
  27. Togari N, Kobayashi A, Aishima T. Relating sensory properties of tea aroma to gas chromatographic data by chemometric calibration methods. Food Res. Int. 28: 485–493 (1995)CrossRefGoogle Scholar
  28. Wang K, Liu F, Liu Z, Huang J, Xu Z, Li Y, Chen J, Gong Y, Yang X. Analysis of chemical components in oolong tea in relation to perceived quality. Int. J. Food Sci. Technol. 45: 913–920 (2010)CrossRefGoogle Scholar
  29. Wiklund S, Johansson E, Sjöström L, Mellerowicz EJ, Edlund U, Shockcor JP, Gottfries J, Moritz T, Trygg J. Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal. Chem. 80: 115–122 (2008)CrossRefGoogle Scholar
  30. Yang Z, Baldermann S, Watanabe N. Recent studies of the volatile compounds in tea. Food Res. Int. 53: 585–599 (2013)CrossRefGoogle Scholar
  31. Yang DS, Lee KS, Jeong OY, Kim KJ, Kays SJ. Characterization of volatile aroma compounds in cooked black rice. J. Agric. Food Chem. 56: 235–240 (2008)CrossRefGoogle Scholar
  32. Zeng L, Zhou Y, Gui J, Fu X, Mei X, Zhen Y, Ye T, Du B, Dong F, Watanabe N, Yang Z. Formation of volatile tea constituent indole during the oolong tea manufacturing process. J. Agric. Food Chem. 64: 5011–5019 (2016)CrossRefGoogle Scholar
  33. Zeng L, Liao Y, Li J, Zhou Y, Tang J, Dong F, Yang Z. α-Farnesene and ocimene induce metabolite changes by volatile signaling in neighboring tea (Camellia sinensis) plants. Plant Sci. 264: 29–36 (2017a)CrossRefGoogle Scholar
  34. Zeng L, Zhou Y, Fu X, Mei X, Cheng S, Gui J, Dong F, Tang J, Ma S, Yang Z. Does oolong tea (Camellia sinensis) made from a combination of leaf and stem smell more aromatic than leaf-only tea? Contribution of the stem to oolong tea aroma. Food Chem. 237: 488–498 (2017b)CrossRefGoogle Scholar
  35. Zhu J, Chen F, Wang L, Niu Y, Xiao Z. Evaluation of the synergism among volatile compounds in Oolong tea infusion by odour threshold with sensory analysis and E-nose. Food Chem. 221: 1484–1490 (2017)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  • Jie Lin
    • 1
  • Yuanxu Shi
    • 2
  • Chunwang Dong
    • 3
  • Xiaochang Wang
    • 2
    Email author
  1. 1.The Key Laboratory for Quality Improvement of Agricultural Product of Zhejiang ProvinceZhejiang A&F UniversityHangzhouPeople’s Republic of China
  2. 2.Institute of Tea ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Tea Research InstituteChinese Academy of Agricultural SciencesHangzhouPeople’s Republic of China

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