Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum
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To facilitate Ganoderma lucidum submerged cultivation and achieve high productivity, four fine powder solid substrates incorporated with different nitrogen-rich supplements were utilized to grow the fungus and as solid seed for its submerged culture. Of the four solid seeds, the soybean meal solid seed gave the highest biomass (10.73 g/L) and exopolysaccharide (EPS) (1.22 g/L), higher than those (8.36 g/L biomass and 0.44 g/L EPS) obtained with mycelial liquid seed. The optimal level of soybean meal supplementation was 20% (w/w) for production of the solid seed. Following single factor experiments, levels of three selected process variables were optimized as: the moisture content of solid seed, 70%; inoculum size, 0.8 g/flask; and rotary speed, 160 rpm. These conditions were validated experimentally with improved EPS yield of 1.33 g/L. The developed solid seed can be conveniently used for G. lucidum submerged culture with improved EPS productivity.
KeywordsGanoderma lucidum Submerged culture Solid seed Exopolysaccharide Response surface methodology
The authors would like to thank the financial support by the Program for Young and Middle School Teachers of Education Department of Fujian Province (Grant No. JA15558), Ningde Normal University (Grant No. 2013F22), and the Industry Guidance Program of the Department of Science and Technology of Fujian Province (Grant No. 2015N0032).
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Conflict of interest
The authors declare that there is no conflict of interest.
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