Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora

  • Sepideh Shariati
  • Davood ZareEmail author
  • Saeed Mirdamadi


The production of many secondary metabolites such as carotenoids is influenced by the type of carbon and nitrogen sources and C:N ratio applied in culture medium. The present study discusses the role of C:N ratio and screening of carbon and nitrogen sources using mixture analysis design in carotenoids production by Blakeslea trispora. The C:N ratios of 20, 40, and 60 with six nitrogen sources were evaluated. Results indicated that limitation of nitrogen source (C:N of 60) could improve carotenoids production. Six nitrogen and carbon sources were then screened using mixture analysis design. The most effective nitrogen and carbon sources were soybean powder and glucose, respectively. The productivity of carotenoids (983.8 ± 31.5 mg/L) based on consumed nitrogen and carbon source was 189.10 mg/g soybean powder and 19.66 mg/g glucose. Mixture analysis design indicated single carbon and nitrogen source were more effective than a mixture of sources for carotenoids production.


Mixture analysis design Carotenoid β-carotene Blakeslea trispora C:N ratio 



The study was financed by a research Grant (No. 94/S/43928) awarded to D. Zare by the Iran National Science Foundation (INSF), Iran.


None of the authors of this study has any financial interest or conflict with industries or parties.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Sepideh Shariati
    • 1
  • Davood Zare
    • 2
    Email author
  • Saeed Mirdamadi
    • 2
  1. 1.Pharmaceutical Sciences BranchIslamic Azad UniversityTehranIslamic Republic of Iran
  2. 2.Department of BiotechnologyIranian Research Organization for Science and Technology (IROST)TehranIslamic Republic of Iran

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