Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora
- 41 Downloads
The production of many secondary metabolites such as carotenoids is influenced by the type of carbon and nitrogen sources and C:N ratio applied in culture medium. The present study discusses the role of C:N ratio and screening of carbon and nitrogen sources using mixture analysis design in carotenoids production by Blakeslea trispora. The C:N ratios of 20, 40, and 60 with six nitrogen sources were evaluated. Results indicated that limitation of nitrogen source (C:N of 60) could improve carotenoids production. Six nitrogen and carbon sources were then screened using mixture analysis design. The most effective nitrogen and carbon sources were soybean powder and glucose, respectively. The productivity of carotenoids (983.8 ± 31.5 mg/L) based on consumed nitrogen and carbon source was 189.10 mg/g soybean powder and 19.66 mg/g glucose. Mixture analysis design indicated single carbon and nitrogen source were more effective than a mixture of sources for carotenoids production.
KeywordsMixture analysis design Carotenoid β-carotene Blakeslea trispora C:N ratio
The study was financed by a research Grant (No. 94/S/43928) awarded to D. Zare by the Iran National Science Foundation (INSF), Iran.
None of the authors of this study has any financial interest or conflict with industries or parties.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Human and animal rights
This article does not contain any studies with human or animal subjects performed by the any of the authors.
- Casas López JL, Sánchez Pérez JA, Fernández Sevilla JM, Acién Fernández FG, Molina Grima E, Chisti Y. Production of lovastatin by Aspergillus terreus: effects of the C:N ratio and the principal nutrients on growth and metabolite production. Enzyme Microb. Tech. 33: 270–277 (2003)CrossRefGoogle Scholar
- Lira GM, Queijeiro Lopez AM, Oliveira Firmino G, Diniz Santos S, Souza Bezerra R. Total carotenoids and antioxidant activity of fillets and shells (in natura or cooked) of “Vila Franca” shrimp (Litopenaeus schmitti) in different intervals of storage under freezing. Ciênc. Agrotec. 41 (1): 94–103 (2017)CrossRefGoogle Scholar
- Roukas T. The role of oxidative stress on carotene production by Blakeslea trispora in submerged fermentation. Crit. Rev. Biotechnol. 1–10 (2015).Google Scholar