Enzymatic characteristics of polyphenoloxidase from shrimp (Penaeus vannamei) and its inhibition by a novel hydroxypyridinone derivative
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Melanosis developed in shrimp (Penaeus vannamei) is mainly initiated by polyphenoloxidase (PPO), thus understanding of the characteristics of PPO in shrimp is important for controlling the melanosis of shrimp. The shrimp cephalothorax turns black most rapidly amongst all the tissues during the chilled storage. Crude PPO extracted from this cephalothorax has an optimal pH of 6.0 and an optimal temperature of 50 °C. PPO is relatively stable under neutral and weak alkaline conditions (pH 5.5–9.0) and the temperature range of 25–35 °C. The kinetic parameters Km and Vmax were recorded as 3.02 mM and 54.3 U/mg of protein, respectively, using L-Dopa as a substrate. The molecular weight of PPO was estimated as 200–220 kDa by an activity staining test. A hydroxypyridinone derivative, 5-hydroxy-1-octyl-4-oxo-1,4-dihydropyridine-2-carbaldehyde O-ethyl oxime, was demonstrated to efficiently inhibit the PPO, indicating that this compound might find application as a shrimp preservative.
KeywordsPolyphenoloxidase Penaeus vannamei Hydroxypyridinone Enzymatic characteristics Inhibitory kinetics
This research work was financially supported by the Natural Science Foundation of Zhejiang Province (No. LY17B020001), and Food Science and Engineering-the most important discipline of Zhejiang Province (2017SIAR216). .
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Conflict of interest
The authors declare that they have no conflict of interest.
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