Food Science and Biotechnology

, Volume 27, Issue 2, pp 581–590 | Cite as

Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441

  • Yoonjung Ju
  • Kwang-Hee Son
  • Chunzhi Jin
  • Byung Soon Hwang
  • Dong-Jin Park
  • Chang-Jin Kim
Article
  • 68 Downloads

Abstract

The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici.

Keywords

Streptomyces rimosus AG-P1441 Phytophthora capsici Biocontrol Medium optimization 

Notes

Acknowledgements

This research was supported by a Grant (10045326) from the R&D Program of MOTIE/KEIT of Republic of Korea, the KRIBB Research Initiative Program, Republic of Korea and a Grant (NRF-2013M3A9A5076601) from a study on the strategies of improving the value of microbial resources funded by Ministry of Science, ICT and Future Planning of the Korea Government.

Supplementary material

10068_2017_257_MOESM1_ESM.docx (445 kb)
Supplementary material 1 (DOCX 445 kb)

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Yoonjung Ju
    • 1
  • Kwang-Hee Son
    • 1
  • Chunzhi Jin
    • 1
    • 2
  • Byung Soon Hwang
    • 1
  • Dong-Jin Park
    • 1
  • Chang-Jin Kim
    • 1
    • 2
  1. 1.Industrial Biomaterial Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  2. 2.University of Science and TechnologyDaejeonKorea

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