Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441
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The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici.
KeywordsStreptomyces rimosus AG-P1441 Phytophthora capsici Biocontrol Medium optimization
This research was supported by a Grant (10045326) from the R&D Program of MOTIE/KEIT of Republic of Korea, the KRIBB Research Initiative Program, Republic of Korea and a Grant (NRF-2013M3A9A5076601) from a study on the strategies of improving the value of microbial resources funded by Ministry of Science, ICT and Future Planning of the Korea Government.
- 4.Zohara F, Akanda AM, Paul NC, Rahman M, Islam T. Inhibitory effects of Pseudomonas spp. on plant pathogen Phytophthora capsici in vitro and in planta. Biocatal. Agric. Biotechnol. 5: 69–77 (2016)Google Scholar
- 7.Crawford DL, Linch JM, Whipps JM and Ousley MA. Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl. Environ. Microbiol. 59: 3899–3905 (1993)Google Scholar
- 10.Kim CJ, Park DJ, Lee JC, Ju YJ, Yoon BS. Development of biocontrol agent from bioactive compounds of microbial origin. Annual report of Rural Development Administration. (2013)Google Scholar
- 12.Grove and Randall. Assay methods of antibiotics medical encyclopedia. Inc. New York, NY, USA. (1955)Google Scholar
- 15.Box G, Hunter W, Hunter J. Statistics for experiments. Wiley, New York, USA. (1958)Google Scholar
- 16.Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi CK. Strategies for Fermentation Medium Optimization: An In-Depth Review. Front. Microbiol. Jan 6;7:2087 (2017)Google Scholar
- 28.Sissi C, Palumbo M. Effects of magnesium and related divalent metal ions in topoisomerase structure and function. Nucleic Acids Res. Feb;37(3): 702–711 (2009)Google Scholar
- 29.Cowan JA. Introduction to the biological chemistry of magnesium ion. The biological chemistry of magnesium. VCH. New York, 1–23 (1995)Google Scholar
- 30.Box, GEP, Wilson, KB. On the experimental attainment of optimum conditions. J. Roy. Stat. Soc. (Ser. B) 13: 1–45 (1951)Google Scholar