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Alterations in peripheral T cell and B cell subsets in patients with osteoarthritis

  • Wanshou Zhu
  • Xi Zhang
  • Yutong Jiang
  • Xiaolian Liu
  • Linyan Huang
  • QiuJing Wei
  • Yefei Huang
  • Weilong Wu
  • Jieruo GuEmail author
Original Article

Abstract

Objectives

Our study objective was to explore whether abnormalities in the subtypes of T cells and B cells were present in peripheral blood of patients with osteoarthritis (OA) and healthy controls (HCs).

Method

Demographic and clinical variables and blood were collected. OA severity was measured using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionnaire. Flow cytometry was used to establish the frequencies of lineage subsets. Monoclonal antibodies against 21 surface markers were used to distinguish and evaluate T cells’ and B cells’ subpopulation. The proportion of each subset was compared and correlations between age, immune cells, and clinical data were analyzed.

Results

A total of 30 OA patients (male/female = 9/21) and 45 HCs (male/female = 14/31) were included. Median WOMAC pain was 3.0 (2.0). There was no difference in the proportion of T cells, CD8+ T cells, and B cells (p > 0.05). The proportion of CD4+ T cells was higher in OA groups, together with an increased CD4 to CD8 ratio (p = 0.016). CD8+CD45RA+ T cells were reduced after adjustment for age, while CD8+CD45RA− T cells were elevated in OA (p < 0.05). CD4+CD45RA−CCR7+ T cells and CD4+CD45RA−CCR7− T cells were increased (p < 0.004). The proportion of T helper (Th) 17 and T follicular helper (Tfh) 2 cells was higher, but cytotoxic T (Tc) 17 cells were fewer in OA (p < 0.05). CD3−CD19+IgD−IgM−CD27+CD38+ B cells were decreased in OA (p ≤ 0.001). The WOMAC pain score correlated with CD3+CD4+CXCR5−PD-1+ T cells positively (B = 0.404, p = 0.027). CD3−CD19+CD27−IgD+ cells correlated negatively with erythrocyte sedimentation rate (ESR) (B = −0.550, p = 0.005).

Conclusions

The T cell and B cell profiles were proved to have alteration suggesting that acquired immune system may play a substantial role in the pathogenesis of OA.

Key Points

• The T cell and B cell profiles were proved to have alteration suggesting that acquired immune system may play a substantial role in the pathogenesis of OA.

• The WOMAC pain score correlated with CD3+CD4+CXCR5−PD-1+ T cells and T helper 17 cells positively.

• Memory T cells were increased in OA patients, suggesting they could play an important role in OA.

Keywords

B cell Lymphocyte Osteoarthritis T cell 

Notes

Acknowledgments

We owed special thanks to Professor Zhinan Yin from Jinan University for his assistance in the study.

Funding information

The study was financially supported by the Municipal Healthcare Joint-Innovation Major Project of Guangzhou (No. 201604020013) and Prevention and Treatment of Multiple Immune Diseases and Allergic Diseases (No. 82000-41070001).

Compliance with ethical standards

The study was approved by the Ethics Commitee of the Third Affiliated Hospital of Sun Yat-sen University ([2018]-02-349-01) and all participants gave a written informed consent

Disclosures

None.

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Copyright information

© International League of Associations for Rheumatology (ILAR) 2019

Authors and Affiliations

  • Wanshou Zhu
    • 1
  • Xi Zhang
    • 2
  • Yutong Jiang
    • 2
  • Xiaolian Liu
    • 1
  • Linyan Huang
    • 1
  • QiuJing Wei
    • 2
  • Yefei Huang
    • 2
  • Weilong Wu
    • 1
  • Jieruo Gu
    • 2
    Email author
  1. 1.Department of RheumatologyGaozhou People’s HospitalGaozhouChina
  2. 2.Department of RheumatologyThe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouPeople’s Republic of China

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