MiR-5571-3p and miR-135b-5p, derived from analyses of microRNA profile sequencing, correlate with increased disease risk and activity of rheumatoid arthritis

  • Cailong Liu
  • Axiao Pan
  • Xiaowei Chen
  • Jianxin Tu
  • Xiaoru XiaEmail author
  • Li SunEmail author
Original Article



This study aimed to investigate microRNA (miRNA) expression profiles in synovium tissues of rheumatoid arthritis (RA) patients by RNA sequencing and to evaluate the values of dysregulated miRNAs in RA diagnosis and monitoring.


Thirty RA patients who underwent knee arthroscopy and 30 controls with knee trauma who underwent surgery were consecutively recruited, and synovium tissue samples of both groups were obtained during surgeries. In the exploration part, miRNA and mRNA expression profiles of 3 RA samples and 3 control samples were detected using RNA sequencing then followed by bioinformatic analyses. In the validation part, 5 candidate miRNA levels were detected by quantitative polymerase chain reaction (qPCR) in 30 RA patients and 30 control patients.


In the exploration part, 78 miRNAs and 1582 mRNAs were upregulated while 40 miRNAs and 1295 mRNAs were downregulated in synovium tissue samples of RA patients compared with those of controls. Furthermore, enrichment analyses revealed that these dysregulated miRNAs and mRNAs were mainly implicated in immune activities and inflammatory diseases such as leukocyte migration, complement activation, and RA. In the validation part, qPCR assay revealed that miR-5571-3p and miR-135b-5p expressions were increased in RA patients compared with those in controls and disclosed good predictive values for RA risk with high area under the curves (AUCs). Besides, both miR-5571-3p and miR-135b-5p levels were positively correlated with disease activity and inflammation level of RA.


Analyses of miRNA expression profiles by sequencing indicate that miR-5571-3p and miR-135b-5p correlate with increased RA risk and activity.


Expression profiles MicroRNA Rheumatoid arthritis RNA sequencing Synovium tissue 


Funding information

This study was approved by the Ethics Review Board of The First Affiliated Hospital of Wenzhou Medical University, and written informed consents of all participants were obtained before enrollment.

Compliance with ethical standards

This study was approved by the Ethics Review Board of The First Affiliated Hospital of Wenzhou Medical University, and all participants provided written informed consents before enrollment.



Supplementary material

10067_2018_4417_MOESM1_ESM.pdf (121 kb)
Supplementary Table 1 (PDF 120 kb)


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Copyright information

© International League of Associations for Rheumatology (ILAR) 2019

Authors and Affiliations

  1. 1.Department of Orthopaedic Sports MedicineThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina
  2. 2.Department of Rheumatology and ImmunologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina

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