, Volume 22, Issue 6, pp 1308–1324 | Cite as

Nitrogen Retention of Terricolous Lichens in a Northern Alberta Jack Pine Forest

  • Adam BirdEmail author
  • Shaun A. Watmough
  • Michael A. Carson
  • Nathan Basiliko
  • Andrew McDonough


The Athabasca Oil Sands in Alberta, Canada, is one of the largest point sources of nitrogen oxides in Canada. There are concerns that elevated nitrogen (N) deposition will adversely impact forest ecosystems located downwind of emission sources. The role of the forest floor in regulating these potential eutrophication effects was investigated following a 5-year enrichment study in which N was applied as NH4NO3 above the canopy of a jack pine (Pinus banksiana Lamb) stand in northern Alberta close to Fort McMurray at rates ranging from 5 to 25 kg N ha−1 y−1 in addition to background deposition of approximately 2 kg N ha−1 y−1. Chemical analysis of lichen mats revealed that the N concentration in the apical (upper) lichen tissue and necrotic tissue increased with treatment. When expressed as a N pool, the fibric–humic material held the largest quantity of N across all treatments due to its relatively large mass (172–214 kg N ha−1), but there was no significant treatment effect. Soil net N mineralization and net nitrification rates did not differ among N treatments after five years of application. A 15N tracer applied to the forest floor showed that N is initially absorbed by the apical lichen (16.6% recovery), FH material (29.4% recovery), and the foliage of the vascular plant Vaccinium myrtilloides (31.7% recovery) in particular. After 2 years, the FH 15N pool size was elevated and all other measured pools were depleted, indicating a slow transfer of N to the FH material. Applied 15N was not detectable in mineral soil. The microbial functional gene ammonia monooxygenase (amoA) responsible for catalyzing the first step in nitrification was undetectable using PCR screening of mineral soil microbial communities in all treatments, and broad fungal/bacterial qPCR assays revealed a weak treatment effect on fungal: bacterial ratios in mineral soil with decreasing relative fungal abundance under higher N deposition. This work suggests that terricolous lichen mats, which form the majority of ground cover in upland jack pine systems, have a large capacity to effectively retain elevated N deposition in soil humus.


nitrogen oil sands eutrophication lichens nitrogen saturation 



This work was funded by CEMA (Cumulative Environmental Management Association). The authors would like to thank our laboratory and field staff at Trent University: Sylvie Danse, Katie Mitchell, Eric Grimm, Liam Murray, Liana Orlovskaya, and Jaqueline London. We would also like to thank our close collaborators Shanti Berryman and Justin Straker of Integral Ecology Group.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Adam Bird
    • 1
    Email author
  • Shaun A. Watmough
    • 2
  • Michael A. Carson
    • 3
  • Nathan Basiliko
    • 3
  • Andrew McDonough
    • 1
  1. 1.Environmental and Life Sciences Graduate ProgramTrent UniversityPeterboroughCanada
  2. 2.Trent School of The EnvironmentTrent UniversityPeterboroughCanada
  3. 3.Department of Biology, Vale Living with Lakes CentreLaurentian UniversitySudburyCanada

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