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Comparison of histomorphometry and microCT after sinus augmentation using xenografts of different particle sizes in rabbits

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Abstract

Purpose

The aim of the present experiment was to compare the data on new bone formation measured histologically and microtomographically in maxillary sinuses augmented with a xenograft with higher density and higher mineral content compared with the natural bone. The hypothesis was that histomorphometric and micro-computed tomography (microCT) analyses do not yield similar outcomes when a xenograft with higher density and mineral content compared with the natural bone is used.

Methods

In 18 rabbits, the maxillary sinus was augmented bilaterally using deproteinized bovine bone mineral (DBBM) xenograft granules of either 0.125–1 mm or 1–2 mm of dimensions. The rabbits were euthanized after 2, 4, and 8 weeks of healing. Comparisons were performed between microCT and histological analyses.

Results

After 2 weeks of healing, higher contents of bone were found at the histological compared with the microCT analyses in both sinuses, especially in the middle regions of the grafted sinus. Between 2 and 8 weeks of healing, new bone increased of about 21% at the histological analyses while, at the microCT, increased only about 4%. In the same period, the xenograft proportion decreased from 51.6 ± 4.9 to 45.3 ± 3.3% at the histological analyses while, at the microCT, the xenograft appeared to increase in percentages.

Conclusion

Histological and microCT analyses yielded different outcomes when a xenograft with higher density and higher mineral content compared with the natural bone was used.

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Acknowledgments

The authors thank the support provided by Erick Ricardo Silva (ERS) for the surgical procedures, Dr. Adriana Luisa Gonçalves de Almeida for the micro CT processing, Dr. Karol Alí Apaza Alccayhuaman (KAAA) for the time spent for the histological and micro CT analyses. We acknowledge the contribution of Mr. Sebastião Blanco for the histological processing.

Funding

ARDEC Academy, Ariminum Odontologica s.r.l., Rimini, Italy provided the economical and scientific support for the experiment.

Author information

Correspondence to Daniele Botticelli.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. The protocol for this study was approved by the Ethical Committee of the Faculty of Dentistry in Ribeirão Preto of the University of São Paulo (USP, SP-Brazil; 2017.1.278.58.9). The study followed the ARRIVE guidelines, as well as the guidelines for animal care used in Brazil.

Informed consent

This study does not contain any experiment with human participants.

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Place where the study was performed: Faculty of Dentistry in Ribeirão Preto of the University of São Paulo

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Iida, T., Baba, S., Botticelli, D. et al. Comparison of histomorphometry and microCT after sinus augmentation using xenografts of different particle sizes in rabbits. Oral Maxillofac Surg 24, 57–64 (2020). https://doi.org/10.1007/s10006-019-00813-x

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Keywords

  • Animal study
  • Sinus floor elevation
  • Bone healing
  • Xenograft
  • MicroCT analysis