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Extremophiles

, Volume 23, Issue 6, pp 649–657 | Cite as

Distinct roles of an ionic interaction holding an alpha-helix with catalytic Asp and a beta-strand with catalytic His in a hyperthermophilic esterase EstE1 and a mesophilic esterase rPPE

  • VinayKumar Dachuri
  • Ngoc Truongvan
  • Quynh DangThu
  • Sei-Heon Jang
  • ChangWoo LeeEmail author
Original Paper
  • 96 Downloads

Abstract

An ionic interaction that holds an α-helix and a β-strand on which catalytic Asp and His residues are located, respectively, is conserved in a hyperthermophilic esterase EstE1 (optimum temperature 70 °C) and a mesophilic esterase rPPE (optimum temperature 50 °C). We investigated the role of an ionic interaction between E258 and R275 in EstE1 and that between E263 and R280 in rPPE in active-site stability of serine esterases adapted to different temperatures. Ala substitutions caused a 5–10 °C decrease in the optimum temperature of both EstE1 and rPPE mutants. Surprisingly, disruption of the ionic interaction caused larger effects on the conformational flexibility of EstE1 mutants despite their rigid structures, whereas the disruption had fewer effects on the thermal stability of EstE1 mutants at 60–70 °C, as the structure of EstE1 was adapted to high temperatures. In contrast, mesophilic rPPE mutants showed dramatic decreases in thermal stability at 40–50 °C, but less changes in conformational flexibility because of their inherently flexible structures. The results of this study suggest that the ionic interaction between the α-helix with catalytic Asp and the β-strand with catalytic His plays an important role in the active-site conformation of EstE1 and rPPE, with larger effects on the conformational flexibility of hyperthermophilic EstE1 and the thermal stability of mesophilic rPPE.

Keywords

Catalytic-site thermal stability Conformational flexibility Ionic interaction Mesophilic esterase Thermophilic esterase 

Abbreviations

pNPA

p-Nitrophenyl acetate

pNPH

p-Nitrophenyl hexanoate

WT

Wild type

Notes

Acknowledgements

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A3B07048635 to C.L.).

Supplementary material

792_2019_1115_MOESM1_ESM.docx (1.7 mb)
Supplementary material 1 (DOCX 1762 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • VinayKumar Dachuri
    • 1
    • 2
  • Ngoc Truongvan
    • 1
  • Quynh DangThu
    • 1
  • Sei-Heon Jang
    • 1
  • ChangWoo Lee
    • 1
    Email author
  1. 1.Department of Biomedical Science and Center for Bio-NanomaterialsDaegu UniversityGyeongsanSouth Korea
  2. 2.Research Center for Herbal Convergence on Liver DiseaseDaegu Haany UniversityGyeongsanSouth Korea

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