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Extremophiles

, Volume 23, Issue 4, pp 461–466 | Cite as

Toward a rapid method for the study of biodiversity in cold environments: the characterization of psychrophilic yeasts by MALDI-TOF mass spectrometry

  • Joseph J. DallugeEmail author
  • Elizabeth C. Brown
  • Laurie B. Connell
Original Paper

Abstract

To investigate the potential of matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) as a platform to support biodiversity and phylogenetic studies of psychrophilic yeasts in cold environments, the technique was employed to rapidly characterize and distinguish three psychrophilic yeasts (Rhodotorula mucilaginosa, Naganishia vishniacii, and Dioszegia cryoxerica) from three mesophilic counterparts (Saccharomyces cerevisiae Cry Havoc, S. cerevisiae California V Ale, and S. pastorianus). A detailed workflow for providing reproducible mass spectral fingerprints of low molecular weight protein/peptide features specific to the organisms studied is presented. The potential of this approach as a tool in the study of biodiversity, systematics, and phylogeny of psychrophilic microorganisms is highlighted.

Keywords

Biodiversity Fungi Arctic Antarctic Yeasts Phylogeny Systematics 

Notes

Acknowledgements

The authors thank Ms. Leslie Astbury for her work in growing the microorganisms for this project.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest.

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Joseph J. Dalluge
    • 1
    Email author
  • Elizabeth C. Brown
    • 1
  • Laurie B. Connell
    • 2
  1. 1.Department of ChemistryUniversity of MinnesotaMinneapolisUSA
  2. 2.School of Marine SciencesUniversity of MaineOronoUSA

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