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Extremophiles

, Volume 22, Issue 6, pp 931–941 | Cite as

Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure

  • Ida Romano
  • Annalisa De Angelis
  • Annarita Poli
  • Pietro Ragni
  • Laura Lilla
  • Gianluigi Zito
  • Barbara Nicolaus
  • Anna Chiara De Luca
  • Paola Di Donato
Original Paper
  • 101 Downloads

Abstract

Spores of the genus Bacillus are able to resist ionizing radiations and therefore they are a suitable biological model for studies in Astrobiology, i.e. the multidisciplinary approach to the study of the origin and evolution of life on Earth and in the universe. The resistance to γ-radiation is an important issue in Astrobiology in relation to the search for bacterial species that could adapt to life in space. This study investigates the resistance of spores of the thermophilic bacteria Parageobacillus thermantarcticus to γ-rays. The analysis of spores’ response to irradiation at a molecular level is performed by means of Raman spectroscopy that allows to get insights in the sequence of events taking place during inactivation. The role of the γ-rays’ dose in the inactivation of spores is also investigated, allowing to highlight the mechanism(s) of inactivation including DNA damage, protein denaturation and calcium dipicolinate levels.

Keywords

Astrobiology Raman spectroscopy Thermophiles γ-rays resistance Bacillus 

Notes

Acknowledgements

Annalisa De Angelis and Ida Romano contributed equally to this work.

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Ida Romano
    • 1
  • Annalisa De Angelis
    • 2
  • Annarita Poli
    • 1
  • Pietro Ragni
    • 3
  • Laura Lilla
    • 3
  • Gianluigi Zito
    • 2
  • Barbara Nicolaus
    • 1
  • Anna Chiara De Luca
    • 2
  • Paola Di Donato
    • 1
    • 4
  1. 1.Institute of Biomolecular ChemistryNational Research Council of ItalyPozzuoli, NaplesItaly
  2. 2.Institute of Protein BiochemistryNational Research Council of ItalyNaplesItaly
  3. 3.Institute of Chemical MethodologiesResearch Area of Rome, National Council Research of ItalyMonterotondo S.Italy
  4. 4.Department of Science and TechnologyUniversity of Naples “Parthenope”, Centro Direzionale Isola C4NaplesItaly

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