Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure
- 55 Downloads
Spores of the genus Bacillus are able to resist ionizing radiations and therefore they are a suitable biological model for studies in Astrobiology, i.e. the multidisciplinary approach to the study of the origin and evolution of life on Earth and in the universe. The resistance to γ-radiation is an important issue in Astrobiology in relation to the search for bacterial species that could adapt to life in space. This study investigates the resistance of spores of the thermophilic bacteria Parageobacillus thermantarcticus to γ-rays. The analysis of spores’ response to irradiation at a molecular level is performed by means of Raman spectroscopy that allows to get insights in the sequence of events taking place during inactivation. The role of the γ-rays’ dose in the inactivation of spores is also investigated, allowing to highlight the mechanism(s) of inactivation including DNA damage, protein denaturation and calcium dipicolinate levels.
KeywordsAstrobiology Raman spectroscopy Thermophiles γ-rays resistance Bacillus
Annalisa De Angelis and Ida Romano contributed equally to this work.
- Coorevits A, Dinsdale AE, Halket G, Lebbe L, De Vos P, Van Landschoot A, Logan NA (2012) Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly ‘thermoglucosidasius’); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov. Int J Syst Evol Microbiol 62(Pt 7):1470–1485CrossRefPubMedGoogle Scholar
- Di Cristo C, Di Donato P, Palumbo A, d’Ischia M, Paolucci M, Di Cosmo A (2010) Steroidogenesis in the brain of Sepia officinalis and Octopus vulgaris. Front Biosci Elite 2:673–683Google Scholar
- Ghosal D, Omelchenko MV, Gaidamakova EK, Matrosova VY, Vasilenko A, Venkateswaran A, Zhai M, Kostandarithes HM, Brim H, Makarova KS, Wackett LP, Fredrickson JK, Daly MJ (2005) How radiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiol Rev 29(2):361–375PubMedGoogle Scholar
- Horneck G, Walter N, Westall F, Grenfell JL, Martin WF, Gomez F, Leuko S, Lee N, Onofri S, Tsiganis K, Saladino R, Pilat-Lohinger E, Palomba E, Harrison J, Rull F, Muller C, Strazzulla G, Brucato JR, Rettberg P, Capria MT (2016) AstRoMap European astrobiology roadmap. Astrobiology 16:201–243CrossRefPubMedPubMedCentralGoogle Scholar
- Ito H, Watanabe H, Takehisa M, Iizuka H (1983) Isolation and identification of radiation-resistant cocci belonging to the genus Deinococcus from sewage sludges and animal feeds. Agric Biol Chem 47:1239–1247Google Scholar
- Managò S, Migliaccio N, Terracciano M, Napolitano M, Martucci NM, De Stefano L, Rendina I, De Luca AC, Lamberti A, Rea I (2018) Internalization kinetics and cytoplasmic localization of functionalized diatomite nanoparticles in cancer cells by Raman imaging. J Biophotonics 11(4):e201700207. https://doi.org/10.1002/jbio.201700207 CrossRefPubMedGoogle Scholar
- Moeller R, Reitz G, Li Z, Klein S, Nicholson WL (2012) Multifactorial resistance of Bacillus subtilis spores to high-energy proton radiation: role of spore structural components and the homologous recombination and non-homologous end joining DNA repair pathways. Astrobiology 12(11):1069–1077CrossRefPubMedPubMedCentralGoogle Scholar
- Nicholson WL, Schuerger AC (2005) Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia. Astrobiology 5(4):536–544CrossRefPubMedGoogle Scholar
- Nickerson K, Bulla L, Mounts T (1975) Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis. J Bacteriol 124(3):1256–1262PubMedPubMedCentralGoogle Scholar
- Nicolaus B, Lama L, Esposito E, Manca MC, Gambacorta A, di Prisco G (1996) “Bacillus thermoantarcticus” sp. nov., from mount Melbourne, Antarctica: a novel thermophilic species. Polar Biol 16(2):101–104Google Scholar