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Extremophiles

, Volume 22, Issue 6, pp 895–902 | Cite as

Effects of cadmium exposure on expression of glutathione synthetase system genes in Acidithiobacillus ferrooxidans

  • Chunli Zheng
  • Li Zhang
  • Minjie Chen
  • Xue Qiang Zhao
  • Yizhuo Duan
  • Ye Meng
  • Xuefeng Zhang
  • Ren Fang Shen
Original Paper
  • 148 Downloads

Abstract

The glutathione synthetase system (GSS) is an important pathway of glutathione synthesis and plays a key role in heavy metal resistance. In this work, the response of Acidithiobacillus ferrooxidans to extracellular Cd2+ was investigated, and the interplay between Cd2+ resistance and the expression of GSS related-genes was analyzed by reverse-transcription quantitative PCR (RT-PCR). During growth in the presence of 5, 15 and 30 mM Cd2+, the transcript levels of eight GSS pathway genes were affected between 0.81- and 7.12-fold. Increased transcription was also reflected in increased enzyme activities: with those of glutathione reductase (GR) increased by 1.10-, 2.26- and 1.54-fold in the presence of 5, 15 and 30 mM Cd2+, respectively. In contrast, the activities of catalase (CAT) and superoxide dismutase (SOD) were decreased in the presence of Cd2+. At the metabolite level, intracellular methane dicarboxylic aldehyde (MDA) content was increased 1.97-, 3.31- and 1.92-fold in the presence of 5, 15 and 30 mM Cd2+, respectively. These results suggest that Cd2+ directly inhibits the activities of CAT and SOD, breaks the redox balance of the cells, which leads to the activation of the other antioxidant pathway of GSS. Resistance of A. ferrooxidans to Cd2+ may involve modulation of expression levels of glutathione S-transferase (GST), GR, and glutathione synthetase, which may protect against oxidative damage.

Keywords

Acidithiobacillus ferrooxidans Cadmium Glutathione Gene expression 

Abbreviations

CAT

Catalase

Cys

Cysteine

DTNB

5,5-dithiobis (2-nitrobenzoic acid)

γ-ECS

Glutamate-cysteine ligase

GR

Glutathione reductase

GS

Glutathione synthetase

GSH

Glutathione

GSS

Glutathione synthetase system

GS-SG

Glutathione disulfide

GST

Glutathione S-transferase

MDA

Methane dicarboxylic aldehyde

ROS

Reactive oxygen species

RT-PCR

Reverse-transcription quantitative PCR

SAP

Sulfur assimilation pathway

SOD

Superoxide dismutase

TBA

Thiobarbituric acid

Notes

Acknowledgements

The project is supported by National Natural Science Foundation of China (51264029, 41561094), Inner Mongolia Natural Science Foundation of China (2017MS0401), Program for Young Talents of Science and Technology in Universities of Inner Mongolia (NJYT-14-B12), Inner Mongolia talent development fund, Inner Mongolia science and technology project (201501083), and Young Innovation Foundation of Inner Mongolia.

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Chunli Zheng
    • 1
    • 2
    • 3
  • Li Zhang
    • 2
  • Minjie Chen
    • 2
  • Xue Qiang Zhao
    • 1
  • Yizhuo Duan
    • 2
  • Ye Meng
    • 2
  • Xuefeng Zhang
    • 2
  • Ren Fang Shen
    • 1
    • 4
  1. 1.State Key Laboratory of Soil and Sustainable AgricultureInstitute of Soil Science, Chinese Academy of SciencesNanjingChina
  2. 2.School of Energy and EnvironmentInner Mongolia University of Science and TechnologyBaotouPeople’s Republic of China
  3. 3.Key Laboratory of Integrated Exploitation of Bayan Obo Multi-Metal ResourcesBaotouPeople’s Republic of China
  4. 4.University of Chinese Academy of SciencesBeijingChina

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