, Volume 22, Issue 6, pp 865–875 | Cite as

Potential of Lentibacillus sp. NS12IITR for production of lipids with enriched branched-chain fatty acids for improving biodiesel properties along with hydrocarbon co-production

  • Noopur Singh
  • Bijan ChoudhuryEmail author
Original Paper


Hypersaline environment is inhabited by array of microbes which have the potential to produce industrially important products. This study explored biomass and lipid production potential of the halophilic bacterium, strain NS12IITR which was isolated from Sambhar Lake, Rajasthan. Sequencing and phylogenetic analysis revealed that the bacterium belonged to genus Lentibacillus. The salient feature of the isolate is its ability to accumulate total cellular lipid up to 18.9 ± 0.45% of dry cell weight. In addition, trans-esterification of extracted lipid yielded 77.6 ± 5.56% of total esters as methyl ester of branched-chain fatty acids (BCFAs). To assess the nature of extracted lipid, lipid sample was fractionated on the silicic acid column, which demonstrated that 49.03 ± 1.35% of the total lipids was neutral in nature. Trans-esterification of the neutral lipid fraction yielded 60.62 ± 4.88% of total esters as methyl ester of BCFAs. Methyl esters of BCFAs were present in trans-esterified products of neutral as well as polar lipid fractions. Furthermore, the isolate produced hydrocarbons both extracellularly (C10–C30) and intra-cellularly (C15–C28). The concentration of extracellular hydrocarbon (21.11 ± 0.78 mg/L) synthesized by strain NS12IITR is in close agreement with the yield reported from other hydrocarbon producing bacteria. This is hereby a first report on the co-production of lipids and hydrocarbon from a halophilic bacterium. The production of neutral lipid with high percentage of BCFAs and co-production of hydrocarbons makes the isolate NS12IITR a potential claimant for biofuel production.


Lentibacillus Branched-chain fatty acids Hydrocarbon Anteiso-pentadecanoic acid Halophiles Biodiesel 



Branched-chain fatty acid


Straight-chain fatty acid 


Sodium chloride


Potassium chloride


Magnesium sulfate


Potassium dihydrogen phosphate


Ferrous sulfate


Fatty acid methyl ester


Hydrochloric acid


Gas chromatography and mass spectrometry


Micro liter






Bacterial acid methyl ester


Micro gram


Gram per liter


Milligram per liter


Saturated fatty acid


Neutral lipid


Glycolipid and sphingolipid




Thin-layer chromatography


Manganese chloride




Distilled water


Sulphuric acid





1,2 DAGs

1,2 diacylglycerols

1,3 DAGs

1,3 diacylglycerols


Free fatty acid


Cholesterol esters


High altitude Andean lake


Tris acetate EDTA


Ethylene diamine tetraacetic acid


Potential of hydrogen




Fatty acid acyl ester


Polymerase chain reaction



The authors gratefully acknowledge the financial support IIT Roorkee and Govt. of India for providing research fellowship.


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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyIIT RoorkeeRoorkeeIndia

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