Microbial community composition and dolomite formation in the hypersaline microbial mats of the Khor Al-Adaid sabkhas, Qatar
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The Khor Al-Adaid sabkha in Qatar is among the rare extreme environments on Earth where it is possible to study the formation of dolomite—a carbonate mineral whose origin remains unclear and has been hypothetically linked to microbial activity. By combining geochemical measurements with microbiological analysis, we have investigated the microbial mats colonizing the intertidal areas of sabhka. The main aim of this study was to identify communities and conditions that are favorable for dolomite formation. We inspected and sampled two locations. The first site was colonized by microbial mats that graded vertically from photo-oxic to anoxic conditions and were dominated by cyanobacteria. The second site, with higher salinity, had mats with an uppermost photo-oxic layer dominated by filamentous anoxygenic photosynthetic bacteria (FAPB), which potentially act as a protective layer against salinity for cyanobacterial species within the deeper layers. Porewater in the uppermost layers of the both investigated microbial mats was supersaturated with respect to dolomite. Corresponding to the variation of the microbial community’s vertical structure, a difference in crystallinity and morphology of dolomitic phases was observed: dumbbell-shaped proto-dolomite in the mats dominated by cyanobacteria and rhombohedral ordered-dolomite in the mat dominated by FAPB.
KeywordsExtreme environment Microbial mat Dolomite precipitation Extracellular polymeric substances Sabkha
This publication was made possible by NPRP Grant 7-443-1-083 from the Qatar National Research Fund (a member of Qatar Foundation). MD was supported by the National Sciences and Engineering Research Council of Canada (NSERC Discovery Grant) and the Canada Foundation for Innovation and Ontario Research Fund (Leaders Opportunity Fund, Grant Number 22404). The statements made herein are solely the responsibility of the authors. The authors would like to acknowledge Oleksandra Kaskun for performing alkalinity measurements, Dr. K. Tait at the Royal Ontario Museum for use of XRD and Sal Boccia at the Ontario Centre for the Characterizations of Advanced Materials (OCCAM) for the assistance with SEM imaging.
All authors contributed to the conception and design of the research. MD, TB, and ZD were responsible for fieldwork. CP participated in fieldwork in March 2016. ZD was responsible for writing the first draft of the manuscript and conducting the laboratory work, and ZD and MD collected the field data. All authors were involved in the interpretation of the data, contributing to sections of the manuscript, as well as revising and approving the final version for submission. All authors agree to be accountable for all aspects of the work and ensuring that questions related to accuracy or integrity of the work are appropriately addressed.
Compliance with ethical standards
Conflict of interest
All of the involved authors have no conflict of interest that would influence the research conducted or conclusions drawn in this manuscript.
All datasets generated or analyzed will be made available by request in a timely manner to any qualified researcher. 16S rRNA sequences were submitted for individual samples to the NCBI SRA database and will be available under accession number SRP159889 upon publication.
- Anderson KL, Tayne TA, Ward DM (1987) Formation and fate of fermentation products in hot spring cyanobacterial mats. Appl Environ Microbiol 53:2343–2352Google Scholar
- Bontognali TR, Al Disi ZA, Mckenzie JA, Strohmenger CJ, Rivers JM, Dittrich M, Sadooni F, Al-Kuwari HAS (2016) Microbial mats from the Khor Al-Adaid sabkha, Qatar: morphotypes and association with authigenic minerals. In: Qatar Foundation Annual Research Conference Proceedings, vol 1. HBKU Press, Qatar, p EEPP2895Google Scholar
- Canfield DE, Kristensen E, Thamdrup B (2005) Carbon fixation and phototrophy. Advances in marine biology, vol 48. Elsevier, New York, pp 95–127Google Scholar
- Edgar RC (2016a) UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing. BioRxiv:081257Google Scholar
- Edgar R (2016b) SINTAX: a simple non-Bayesian taxonomy classifier for 16S and ITS sequences. BioRxiv:074161Google Scholar
- Edgar RC (2018) Updating the 97% identity threshold for 16S ribosomal RNA OTUs. Bioinformatics 1:5Google Scholar
- Hammer Ø, Harper D, Ryan P (2001) PAST-palaeontological statistics. http://www.uves/~pardomv/pe/2001_1/past/pastprog/past.pdf, acessadoem, p 25
- Illing LV, Taylor JC (1993) Penecontemporaneous dolomitization in Sabkha Faishakh, Qatar; evidence from changes in the chemistry of the interstitial brines. J Sediment Res 63:1042–1048Google Scholar
- Illing L, Wells A, Taylor J (1965) Penecontemporary dolomite in the Persian Gulf. In: SEPM special publications 13: Dolomitization and Limestone DiagenesisGoogle Scholar
- Imhoff JF, Hiraishi A, Süling J (2015) Anoxygenic phototrophic purple bacteria. In: Bergey’s manual of systematics of archaea and bacteria, p 1–23Google Scholar
- Javor BJ (2012) Hypersaline environments: microbiology and biogeochemistry. Springer, BerlinGoogle Scholar
- Jørgensen BB, Revsbech NP, Blackburn TH, Cohen Y (1979) Diurnal cycle of oxygen and sulfide microgradients and microbial photosynthesis in a cyanobacterial mat sediment. Appl Environ Microbiol 38:46–58Google Scholar
- Keppen OI, Tourova TP, Kuznetsov BB, Ivanovsky RN, Gorlenko VM (2000) Proposal of Oscillochloridaceae fam. nov. on the basis of a phylogenetic analysis of the filamentous anoxygenic phototrophic bacteria, and emended description of Oscillochloris and Oscillochloris trichoides in comparison with further new isolates. Int J Syst Evol Microbiol 50:1529–1537CrossRefGoogle Scholar
- McKenzie JA (1991) The dolomite problem: an outstanding controversy controversies in modern geology: evolution of geological theories. Sedimentology. Academic Press, London, pp 37–54Google Scholar
- Overmann J (2008) Green nonsulfur bacteria. Encyclopedia of life sciences. Wiley, London, pp 1–10Google Scholar
- Parkhurst DL, Appelo C (2013) Description of input and examples for PHREEQC version 3–a computer program for speciation, batch-reaction, one-dimensional transport, and inverse geochemical calculationsGoogle Scholar
- Pierson BK (2001) Phylum BVI. Chloroflexiphy. nov. Family I. “Chloroflexaceae”. Bergey’s manual of systematic bacteriology, 2nd edn. Springer, Berlin, pp 427–429Google Scholar
- Strohmenger CJ, Al-Mansoori A, Al-Jeelani O, Al-Shamry A, Al-Hosani I, Al-Mehsin K, Shebl H (2010) The sabkha sequence at Mussafah Channel (Abu Dhabi, United Arab Emirates): facies stacking patterns, microbial-mediated dolomite and evaporite overprint. GeoArabia 15:49–90Google Scholar
- Strohmenger CJ, Shebl H, Al-Mansoori A, Al-Mehsin K, Al-Jeelani O, Al-Hosani I, Al-Shamry A, Al-Baker S (2011) Facies stacking patterns in a modern arid environment: a case study of the Abu Dhabi sabkha in the vicinity of Al-Qanatir Island, United Arab Emirates. In: Alsharhan A, Kendall CGC (eds) Quaternary carbonate and evaporite sedimentary facies and their ancient analogues: a tribute to Douglas James Shearman. International Association of Sedimentologists, Special Publication, Al-Qanatir, pp 149–182Google Scholar
- Stumm W, Morgan JJ (1996) Aquatic chemistry: chemical equilibria and rates in natural waters. Wiley, New YorkGoogle Scholar
- Trichet J, Defarge C (1995) Non-biologically supported organomineralization. Bulletin-Institut Oceanographique, Momaco-Numero, pp 203–236Google Scholar
- Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer K-H, Whitman WB (2011a) Bergey’s manual of systematic bacteriology: volume 2: the proteobacteria. Springer, BerlinGoogle Scholar
- Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer K-H, Whitman WB (2011b) Bergey’s manual of systematic bacteriology: volume 3: the firmicutes. Springer, BerlinGoogle Scholar
- Ward DM, Ferris MJ, Nold SC, Bateson MM (1998) A natural view of microbial biodiversity within hot spring cyanobacterial mat communities. Microbiol Mol Biol Rev 62:1353–1370Google Scholar
- Ward DM, Klatt CG, Wood J, Cohan FM, Bryant DA (2012) Functional genomics in an ecological and evolutionary context: maximizing the value of genomes in systems biology. Functional genomics and evolution of photosynthetic systems. Springer, Dordecht, pp 1–16Google Scholar
- Warren JK (2016) Hydrocarbons and evaporites. Evaporites. Springer, Berlin, pp 959–1079Google Scholar
- Zempolich WG, Baker PA (1993) Experimental and natural mimetic dolomitization of aragonite ooids. J Sediment Res 63:596–606Google Scholar