Improving bonding to eroded dentin by using collagen cross-linking agents: 2 years of water storage
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The aim of this study was to investigate the effects of collagen cross-linking agents on nanomechanical and bonding properties of eroded dentin (ED), 24 h and 2 years after water storage.
Materials and methods
Human molar dentin surfaces, eroded by soft drinks or citric acid, were acid-etched and treated with primers containing proanthocyanidin (PA) and riboflavin (RI) or were untreated (control) and tested after 24 h and 2 years. After acid etching and adhesive application (Prime&Bond Elect (PBE); Scotchbond Universal (SBU); Tetric n-bond Universal (TEU)), specimens were sectioned into beams and tested for microtensile bond strength (μTBS) and silver nitrate deposition (NL) after 24 h and 2 years. The beams were used to evaluate the 24-h in situ conversion of degree (DC). Nanohardness (NH) and Young’s modulus (YM) were evaluated via resin-bonded dentin slices after 24 h and 2 years. A three-way ANOVA and Tukey’s test were used for statistical analysis (5%).
For both storage times, ED with citric acid resulted in lower μTBS, NH, and YM and higher NL for each adhesive system than soft drink ED (p < 0.05). After 2 years of water storage, cross-linking primers maintained the μTBS, NH, and YM (p < 0.05) when compared with the control group. Althougth, the NL values decreased for all groups after 2 years of water storage, PA and RI treatments showed NI values lower than control group (p < 0.001). No significant differences were observed between PA and RI treatments (p > 0.05). Cross-linking primers maintain or improve DC (p < 0.03). In general, TEU and SBU yielded higher μTBS, DC, NH, and YM and lower NL than PBE.
Cross-linking agents improved the results and maintained the resin-ED interface bonding and nanomechanical properties, without jeopardizing adhesive polymerization.
Cross-linking agents are a viable alternative for improving and maintaining resin-ED interface bonding and nanomechanical properties.
KeywordsProanthocyanidins Tooth erosion Riboflavin Adhesive systems Microtensile bond strength Hardness tests
This study was performed by Fabiana Siqueira as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 303332/2017-4 and 304105/2013-9 and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Also, authors are grateful for the support provided by Cental de Laboratórios Multiusuários/Universidade Estadual de Ponta Grossa (CLABMU/UEPG).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants are in accordance with the ethical standards of the State University of Ponta Grossa research committee. This study was approved by the State University of Ponta Grossa ethics committee.
Informed consent was not obtained because there were no human participants included in the study.
- 6.Meurman JH, Drysdale T, Frank RM (1991) Experimental erosion of dentin. Scand J Dent Res 99:457–462Google Scholar
- 21.Francisconi-dos-Rios LF, Calabria MP, Casas-Apayco LC, Honorio HM, Carrilho MR, Pereira JC, Wang L (2015) Chlorhexidine does not improve but preserves bond strength to eroded dentin. Am J Dent 28:28–32Google Scholar
- 24.Hass V, de Paula AM, Parreiras S, Gutierrez MF, Luque-Martinez I, de Paris Matos T, Bandeca MC, Loguercio AD, Yao X, Wang Y, Reis A (2016) Degradation of dentin-bonded interfaces treated with collagen cross-linking agents in a cariogenic oral environment: an in situ study. J Dent 49:60–67. https://doi.org/10.1016/j.jdent.2016.02.009 CrossRefGoogle Scholar
- 25.Hass V, Luque-Martinez IV, Gutierrez MF, Moreira CG, Gotti VB, Feitosa VP, Koller G, Otuki MF, Loguercio AD, Reis A (2016) Collagen cross-linkers on dentin bonding: stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition. Dent Mater 32:732–741. https://doi.org/10.1016/j.dental.2016.03.008 CrossRefGoogle Scholar
- 31.Cruz JB, Bonini G, Lenzi TL, Imparato JC, Raggio DP (2015) Bonding stability of adhesive systems to eroded dentin. Braz Oral Res 29:1–6. https://doi.org/10.1590/1807-3107BOR-2015.vol29.0088 CrossRefGoogle Scholar
- 32.Forgerini TV, Ribeiro JF, Rocha RO, Soares FZ, Lenzi TL (2017) Role of etching mode on bonding longevity of a universal adhesive to eroded dentin. J Adhes Dent. https://doi.org/10.3290/j.jad.a37723
- 41.Reis A, Loguercio AD, Azevedo CL, de Carvalho RM, da Julio Singer M, Grande RH (2003) Moisture spectrum of demineralized dentin for adhesive systems with different solvent bases. J Adhes Dent 5:183–192Google Scholar
- 43.Hass V, Dobrovolski M, Zander-Grande C, Martins GC, Gordillo LA, Rodrigues Accorinte Mde L, Gomes OM, Loguercio AD, Reis A (2013) Correlation between degree of conversion, resin-dentin bond strength and nanoleakage of simplified etch-and-rinse adhesives. Dent Mater 29:921–928. https://doi.org/10.1016/j.dental.2013.05.001 CrossRefGoogle Scholar
- 45.Urabe I, Nakajima S, Sano H, Tagami J (2000) Physical properties of the dentin-enamel junction region. Am J Dent 13:129–135Google Scholar
- 48.Pashley DH, Ciucchi B, Sano H, Horner JA (1993) Permeability of dentin to adhesive agents. Quintessence Int 24:618–631Google Scholar
- 50.Frassetto A, Breschi L, Turco G, Marchesi G, Di Lenarda R, Tay FR, Pashley DH, Cadenaro M (2016) Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability--a literature review. Dent Mater 32:e41–e53. https://doi.org/10.1016/j.dental.2015.11.007 CrossRefGoogle Scholar
- 52.Daood U, Swee Heng C, Neo Chiew Lian J, Fawzy AS (2015) In vitro analysis of riboflavin-modified, experimental, two-step etch-and-rinse dentin adhesive: Fourier transform infrared spectroscopy and micro-Raman studies. Int J Oral Sci 7:110–124. https://doi.org/10.1038/ijos.2014.49 CrossRefGoogle Scholar
- 61.Hagerman AE, Klucher KM (1986) Tannin-protein interactions. Prog Clin Biol Res 213:67–76Google Scholar
- 73.Platt JA, Almeida J, Gonzalez-Cabezas C, Rhodes B, Moore BK (2001) The effect of double adhesive application on the shear bond strength to dentin of compomers using three one-bottle adhesive systems. Oper Dent 26:313–317Google Scholar