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Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

  • Priyadarshni BindalEmail author
  • Nareshwaran Gnanasegaran
  • Umesh Bindal
  • Nazmul Haque
  • Thamil Selvee Ramasamy
  • Wen Lin Chai
  • Noor Hayaty Abu Kasim
Original Article
  • 41 Downloads

Abstract

Objective

In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-rich plasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.

Materials and methods

Lipopolysaccharide (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated with different concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay. Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array. Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.

Results

Viability of iDPSCs was significantly (p < 0.05) higher in 20% HPL-supplemented media compared to iDPSCs. Expression of 10 out of 12 selected angiogenic genes, four out of seven adhesion molecules, and seven out of nine cytokine-producing genes were significantly (p < 0.05) higher in cells maintained in 20% HPL-supplemented media compared to that in FBS-supplemented media. Furthermore, expression of all the selected growth factors was significantly higher (p < 0.05) in the supernatants from 20% HPL media at 12 and 24 h post-incubation.

Conclusion

This study suggests that 20% HPL could be optimum to stimulate angiogenesis-related factors in iDPSCs while maintaining their viability.

Clinical relevance

This data may suggest the potential use of 20% HPL for expanding DPSCs scheduled for clinical trials for regenerative therapies including dental pulp regeneration.

Keywords

Cytokines Growth factors Platelet lysate Platelet-rich plasma Viability 

Notes

Funding

This study was funded by High Impact Research MOHE Grant UM.C/HIR/MOHE/DENT/05 from the Ministry of Higher Education Malaysia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Priyadarshni Bindal
    • 1
    • 2
    Email author
  • Nareshwaran Gnanasegaran
    • 2
    • 3
  • Umesh Bindal
    • 4
  • Nazmul Haque
    • 5
  • Thamil Selvee Ramasamy
    • 6
  • Wen Lin Chai
    • 2
  • Noor Hayaty Abu Kasim
    • 2
  1. 1.Centre for Rural HealthSchool of Health Sciences University of TasmaniaLauncestonAustralia
  2. 2.Department of Restorative Dentistry, Faculty of DentistryUniversity of MalayaKuala LumpurMalaysia
  3. 3.Institute of Molecular and Cell Biology (IMCB)Agency for Science Technology and Research (A*STAR)ProteosSingapore
  4. 4.Faculty of Health and Medical SciencesTaylor’s UniversitySubang JayaMalaysia
  5. 5.Faculty of DentistryMAHSA UniversityJenjaromMalaysia
  6. 6.Department of Molecular Medicine, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia

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