Yes-associated protein (YAP) molecule expression in odontogenic epithelium of ameloblastoma

  • Rahul AnandEmail author
  • Sachin C. Sarode
  • Gargi S. Sarode
  • Ladu Singh Rajpurohit
  • Shankargouda Patil
Original Article



YAP (Yes-associated protein molecule) augments multiple processes known to be important for tumor progression and metastasis. Hence, the application of YAP as a targeted therapy for several malignancies has been documented in the literature. Ameloblastoma being a locally invasive odontogenic lesion causes tissue destruction and presents with a great chance of recurrence. Based on these qualities, we hypothesized that YAP could exhibit increased expression in the odontogenic epithelium of ameloblastoma. Therefore, the objective of the present study was to evaluate the immunohistochemical expression of YAP in ameloblastoma and compare the immunoreactivity of YAP in ameloblastoma and dental follicle.

Materials and methods

Twenty-four paraffin-embedded tissue blocks of ameloblastoma and dental follicle each were retrieved from the departmental archives. Immunohistochemical (IHC) staining was performed employing YAP antibody on 5-μm thick sections. The expression of YAP was reviewed on the peripheral lining of ameloblast-like cells and centrally placed stellate reticulum–like cells and was further graded as negative, weak, or strong. For data analysis, Chi-square test and Fisher’s exact test were applied.


In ameloblastoma, peripheral/basal cells exhibited strong expression for YAP in 12/24 cases and weak expression in the other 12 cases. Whereas, 20 cases of dental follicle presented negative expression and only four cases exhibited weak expression (p < 0.001). On statistical analysis, the differences in expression of YAP in stellate cells of ameloblastoma and dental follicle were found to be significant (p < 0.001).


YAP expression was strongly seen in ameloblastoma as compared to the epithelial islands of dental follicle. The neoplastic nature of ameloblastoma could be attributed to YAP molecule and it may play a possible role in tumor invasiveness.

Clinical relevance

Positive YAP expression in ameloblastoma and negative expression in the odontogenic epithelial islands of dental follicle insinuate that YAP could be responsible for the aggressiveness and local tissue invasion commonly encountered with ameloblastoma.


Ameloblastoma Dental follicle Immunohistochemistry Odontogenic tumor Yes-associated protein 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

For the present study, informed consent is not required.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and HospitalDr. D.Y. Patil VidyapeethPuneIndia
  2. 2.Department of Public Health Dentistry, Dr. D. Y. Patil Dental College and HospitalDr. D.Y. Patil VidyapeethPuneIndia
  3. 3.Department of Diagnostic Sciences, Division of Oral Pathology, College of DentistryJazan UniversityJazanKingdom of Saudi Arabia

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