Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats
To evaluate intermittent teriparatide therapy’s influence on the alveolar healing process in rats with induced osteoporosis.
Materials and methods
Fifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42 days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p < 0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496 mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p < 0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p > 0.05).
Treatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals.
Cases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features.
KeywordsOsteoporosis Orchiectomy Teriparatide Men Wnt proteins Alkaline phosphatase
The work was supported by the São Paulo Research Foundation (FAPESP), process number: 2015/14688-0 and 2015/20203-9.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed, process number: 706/2015 of the Brazilian College of Animal Experimentation – COBEA.
For this type of study, formal consent is not required.
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