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Clinical Oral Investigations

, Volume 23, Issue 3, pp 1197–1208 | Cite as

Zn-containing polymer nanogels promote cervical dentin remineralization

  • Manuel Toledano
  • Inmaculada Cabello
  • Estrella Osorio
  • Fátima S. Aguilera
  • Antonio Luis Medina-Castillo
  • Manuel Toledano-OsorioEmail author
  • Raquel Osorio
Original Article
  • 220 Downloads

Abstract

Objective

Nanogels designing for effective treatment of eroded cervical dentin lesions.

Materials and methods

Polymethylmetacrylate-based nanoparticles (NPs) were doxycycline (D), calcium, or zinc loaded. They were applied on eroded cervical dentin. Treated surfaces were characterized morphologically by atomic force and scanning electron microscopy, mechanically probed by a nanoindenter to test nanohardness and Young’s modulus, and chemically analyzed by Raman spectroscopy at 24 h and 7 days of storage. Data were submitted to ANOVA and Student-Newman-Keuls multiple comparisons tests.

Results

Dentin treated with Zn-NPs attained the highest nanomechanical properties, mineralization, and crystallinity among groups. Nanoroughness was lower in Zn-treated surfaces in comparison to dentin treated with undoped gels. Dentin treated with Ca-NPs created the minimal calcification at the surface and showed the lowest Young’s modulus at peritubular dentin. Intertubular dentin appeared remineralized. Dentinal tubules were empty in samples treated with D-NPs, partially occluded in cervical dentin treated with undoped NPs and Ca-NPs, and mineral covered when specimens were treated with Zn-NPs.

Conclusions

Zn-loaded NPs permit functional remineralization of eroded cervical dentin. Based on the tested nanomechanical and chemical properties, Zn-based nanogels are suitable for dentin remineralization.

Clinical relevance

The ability of zinc-loaded nanogels to promote dentin mineralization may offer new strategies for regeneration of eroded cervical dentin and effective treatment of dentin hypersensitivity.

Keywords

Remineralization Calcium Zinc Nanoparticles Cervical dentin 

Notes

Funding

This study was funded by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER), grant numbers MAT2014-52036-P and MAT2017-85999-P. Authors do not have a financial relationship with the organization that sponsored the research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in the present study, involving human participants, were in accordance with the ethical standards of the institutional research committee (405/CEIH/2017) and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Bartlett DW, Shah P (2006) A critical review of non-carious cervical (wear) lesions and the role of abfraction, erosion, and abrasion. J Dent Res 85:306–312.  https://doi.org/10.1177/154405910608500405 CrossRefGoogle Scholar
  2. 2.
    West N, Seong J, Davies M (2014) Dentine hypersensitivity. Monogr Oral Sci 25:108–122.  https://doi.org/10.1159/000360749 CrossRefGoogle Scholar
  3. 3.
    Marshall GW, Marshall SJ, Kinney JH, Balooch M (1997) The dentin substrate: structure and properties related to bonding. J Dent 25:441–458.  https://doi.org/10.1016/S0300-5712(96)00065-6 CrossRefGoogle Scholar
  4. 4.
    Kinney JH, Marshall SJ, Marshall GW (2003) The mechanical properties of human dentin: a critical review and re-evaluation of the dental literature. Crit Rev Oral Biol Med 14:13–29CrossRefGoogle Scholar
  5. 5.
    Lussi A, Schlueter N, Rakhmatullina E, Ganss C (2011) Dental erosion—an overview with emphasis on chemical and histopathological aspects. Caries Res 45:2–12.  https://doi.org/10.1159/000325915 CrossRefGoogle Scholar
  6. 6.
    Buzalaf MA, Charone S, Tjäderhane L (2015) Role of host-derived proteinases in dentine caries and erosion. Caries Res 49:30–37.  https://doi.org/10.1159/000380885 CrossRefGoogle Scholar
  7. 7.
    Kato MT, Leite AL, Hannas AR, Buzalaf MA (2010) Gels containing MMP inhibitors prevent dental erosion in situ. J Dent Res 89:468–472.  https://doi.org/10.1177/0022034510363248 CrossRefGoogle Scholar
  8. 8.
    Gandolfi MG, Iacono F, Pirani C, Prati C (2012) The use of calcium-silicate cements to reduce dentine permeability. Arch Oral Biol 57:1054–1061.  https://doi.org/10.1016/jarchoralbio201202024 CrossRefGoogle Scholar
  9. 9.
    Wang Z, Jiang T, Sauro S, Pashley DH, Toledano M, Osorio R, Liang S, Xing W, Sa Y, Wang Y (2011) The dentine remineralization activity of a desensitizing bioactive glass-containing toothpaste: an in vitro study. Aust Dent J 56:372–381.  https://doi.org/10.1111/j1834-7819201101361x CrossRefGoogle Scholar
  10. 10.
    Gandolfi MG, Taddei P, Siboni F, Modena E, De Stefano ED, Prati C (2011) Biomimetic remineralization of human dentin using promising innovative calcium-silicate hybrid “smart” materials. Dent Mater 27:1055–1069.  https://doi.org/10.1016/jdental201107007 CrossRefGoogle Scholar
  11. 11.
    Osorio R, Alfonso-Rodríguez CA, Medina-Castillo AL, Alaminos M, Toledano M (2016) Bioactive polymeric nanoparticles for periodontal therapy. PLoS One 11:e0166217.  https://doi.org/10.1371/journal.pone.0166217 CrossRefGoogle Scholar
  12. 12.
    Smith AJ, Scheven BA, Takahashi Y, Ferracane JL, Shelton RM, Cooper PR (2012) Dentine as a bioactive extracellular matrix. Arch Oral Biol 57:109–121.  https://doi.org/10.1016/jarchoralbio201107008 CrossRefGoogle Scholar
  13. 13.
    Niu LN, Zhang W, Pashley DH, Breschi L, Mao J, Chen JH, Tay FR (2014) Biomimetic remineralization of dentin. Dent Mater 30:77–96.  https://doi.org/10.1016/jdental201307013 CrossRefGoogle Scholar
  14. 14.
    Cartwright RB (2014) Dentinal hypersensitivity: a narrative review. Community Dent Health 31:15–20.  https://doi.org/10.1922/CDH_3287Cartwright06 Google Scholar
  15. 15.
    Toledano M, Osorio R, Osorio E, Medina-Castillo AL, Toledano-Osorio M, Aguilera FS (2017) Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface. J Mech Behav Biomed Mater 68:62–79.  https://doi.org/10.1016/jjmbbm201701026 CrossRefGoogle Scholar
  16. 16.
    Mayer I, Apfelbaum F, Featherstone JD (1994) Zinc ions in synthetic carbonated hydroxyapatites. Arch Oral Biol 39:87–90.  https://doi.org/10.1016/0003-9969(94)90040-X CrossRefGoogle Scholar
  17. 17.
    Agarwal A, Bhattacharya HS, Srikanth G, Singh A (2013) Comparative evaluation of decalcified freeze dried bone allograft with and without local doxycycline in non-contained human periodontal infrabony defects. J Indian Soc Periodontol 17:490–494.  https://doi.org/10.4103/0972-124X.118322 CrossRefGoogle Scholar
  18. 18.
    Balooch M, Habelitz S, Kinney JH, Marshall SJ, Marshall GW (2008) Mechanical properties of mineralized collagen fibrils as influenced by demineralization. J Struct Biol 162:404–410.  https://doi.org/10.1016/jjsb200802010 CrossRefGoogle Scholar
  19. 19.
    Poon B, Rittel D, Ravichandran G (2008) An analysis of nanoindentation in linearly elastic solids. Int J Solids Struct 45:6018–6033.  https://doi.org/10.1016/jijsolstr200807021 CrossRefGoogle Scholar
  20. 20.
    Kunstar A, Leijten J, van Leuveren S, Hilderink J, Otto C, van Blitterswijk CA, Karperien M, van Apeldoorn AA (2012) Recognizing different tissues in human fetal femur cartilage by label-free Raman microspectroscopy. J Biomed Opt 116012:17.  https://doi.org/10.1117/1JBO1711116012 Google Scholar
  21. 21.
    Toledano M, Aguilera FS, Osorio E, Cabello I, Toledano-Osorio M, Osorio R (2015) Self-etching zinc-doped adhesives improve the potential of caries-affected dentin to be functionally remineralized. Biointerphases 031002:10.  https://doi.org/10.1116/14926442 Google Scholar
  22. 22.
    Wang L, Zhao Y, Mei L, Yu H, Muhammad I, Pan Y, Huang S (2017) Effect of application time of maleic acid on smear layer removal and mechanical properties of root canal dentin. Acta Odontol Scand 75:59–66.  https://doi.org/10.1080/0001635720161248789 CrossRefGoogle Scholar
  23. 23.
    Medina-Castillo AL, Fernandez-Sanchez JF, Segura-Carretero A, Fernandez-Gutierrez A (2010) Micrometer and submicrometer particles prepared by precipitation polymerization: thermodynamic model and experimental evidence of the relation between flory’s parameter and particle size. Macromolecules 43:5804–5813.  https://doi.org/10.1021/ma100841c CrossRefGoogle Scholar
  24. 24.
    Osorio R, Cabello I, Medina-Castillo AL, Osorio E, Toledano M (2016) Zinc-modified nanopolymers improve the quality of resin-dentin bonded interfaces. Clin Oral Investig 20:2411–2420.  https://doi.org/10.1007/s00784-016-1738-y CrossRefGoogle Scholar
  25. 25.
    Saeki K, Marshall GW, Gansky SA, Parkinson CR, Marshall SJ (2016) Strontium effects on root dentin tubule occlusion and nanomechanical properties. Dent Mater 32:240–251.  https://doi.org/10.1016/j.dental.2015.11.020 CrossRefGoogle Scholar
  26. 26.
    Ryou H, Niu LN, Dai L, Pucci CR, Arola DD, Pashley DH, Tay FR (2011) Effect of biomimetic remineralization on the dynamic nanomechanical properties of dentin hybrid layers. J Dent Res 90:1122–1128.  https://doi.org/10.1177/0022034511414059 CrossRefGoogle Scholar
  27. 27.
    Toledano M, Sauro S, Cabello I, Watson T, Osorio R (2013) A Zn-doped etch-and-rinse adhesive may improve the mechanical properties and the integrity at the bonded-dentin interface. Dent Mater 29:e142–e152.  https://doi.org/10.1016/j.dental.2013.04.024 CrossRefGoogle Scholar
  28. 28.
    Han L, Grodzinsky AJ, Ortiz C (2011) Nanomechanics of the cartilage extracellular matrix. Annu Rev Mater Res 41:133–168.  https://doi.org/10.1146/annurev-matsci-062910-100431 CrossRefGoogle Scholar
  29. 29.
    Oliver WC, Pharr GM (1992) An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. J Mater Res 7:1564–1583.  https://doi.org/10.1557/JMR19921564 CrossRefGoogle Scholar
  30. 30.
    Ager JW, Nalla RK, Breeden KL, Ritchie RO (2005) Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone. J Biomed Opt 034012:10.  https://doi.org/10.1117/11924668 Google Scholar
  31. 31.
    Timlin JA, Carden A, Morris MD, Rajachar RM, Kohn DH (2000) Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone. Anal Chem 72:2229–2236.  https://doi.org/10.1021/ac9913560 CrossRefGoogle Scholar
  32. 32.
    Toledano M, Osorio R, Osorio E, García-Godoy F, Toledano-Osorio M, Aguilera FS (2016) Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface. J Mech Behav Biomed Mater 62:247–267.  https://doi.org/10.1016/j.jmbbm.2016.05.013 CrossRefGoogle Scholar
  33. 33.
    McKee MD, Nakano Y, Masica DL, Gray JJ, Lemire I, Heft R, Whyte MP, Crine P, Millán JL (2011) Enzyme replacement therapy prevents dental defects in a model of hypophosphatasia. J Dent Res 90:470–476.  https://doi.org/10.1177/0022034510393517 CrossRefGoogle Scholar
  34. 34.
    Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Marshall GW (2009) Biomechanical perspective on the remineralization of dentin. Caries Res 43:70–77.  https://doi.org/10.1159/000201593 CrossRefGoogle Scholar
  35. 35.
    Zurick KM, Qin C, Bernards MT (2013) Mineralization induction effects of osteopontin, bone sialoprotein, and dentin phosphoprotein on a biomimetic collagen substrate. J Biomed Mater Res A 101:1571–1581.  https://doi.org/10.1002/jbma34462 CrossRefGoogle Scholar
  36. 36.
    Jena A, Shashirekha G (2015) Comparison of efficacy of three different desensitizing agents for in-office relief of dentin hypersensitivity: a 4 weeks clinical study. J Conserv Dent 18:389–393.  https://doi.org/10.4103/0972-0707164052 CrossRefGoogle Scholar
  37. 37.
    Yoshizaki KT, Francisconi-Dos-Rios LF, Sobral MA, Aranha AC, Mendes FM, Scaramucci T (2017) Clinical features and factors associated with non-carious cervical lesions and dentin hypersensitivity. J Oral Rehabil 44:112–118.  https://doi.org/10.1111/joor12469 CrossRefGoogle Scholar
  38. 38.
    Lynch E, Brauer DS, Karpukhina N, Gillam DG, Hill RG (2012) Multi-component bioactive glasses of varying fluoride content for treating dentin hypersensitivity. Dent Mater 28:168–178.  https://doi.org/10.1016/jdental201111021 CrossRefGoogle Scholar
  39. 39.
    Salehi H, Terrer E, Panayotov I, Levallois B, Jacquot B, Tassery H, Cuisinier F (2013) Functional mapping of human sound and carious enamel and dentin with Raman spectroscopy. J Biophotonics 6:765–774.  https://doi.org/10.1002/jbio201200095 Google Scholar
  40. 40.
    Schwartz AG, Pasteris JD, Genin GM, Daulton TL, Thomopoulos S (2012) Mineral distributions at the developing tendon enthesis. PLoS One e48630:7.  https://doi.org/10.1371/journalpone0048630 Google Scholar
  41. 41.
    Wang C, Wang Y, Huffman NT, Cui C, Yao X, Midura S, Midura RJ, Gorski JP (2009) Confocal laser Raman microspectroscopy of biomineralization foci in UMR 106 osteoblastic cultures reveals temporally synchronized protein changes preceding and accompanying mineral crystal deposition. J Biol Chem 284:7100–7113.  https://doi.org/10.1074/jbcM805898200 CrossRefGoogle Scholar
  42. 42.
    Osorio R, Osorio E, Cabello I, Toledano M (2014) Zinc induces apatite and scholzite formation during dentin remineralization. Caries Res 48:276–290.  https://doi.org/10.1159/000356873 CrossRefGoogle Scholar
  43. 43.
    Xu C, Wang Y (2012) Collagen cross linking increases its biodegradation resistance in wet dentin bonding. J Adhes Dent 14:11–18.  https://doi.org/10.3290/jjada21494 Google Scholar
  44. 44.
    Bakland LK, Andreasen JO (2012) Will mineral trioxide aggregate replace calcium hydroxide in treating pulpal and periodontal healing complications subsequent to dental trauma? A review. Dent Traumatol 28:25–32.  https://doi.org/10.1111/j1600-9657201101049x CrossRefGoogle Scholar
  45. 45.
    Hannas AR, Zarella BL, Charone S, Taioki V, Kato MT, Tjäderhane L, Buzalaf MAR (2014) Dentin erosion prevention by matrix metalloproteinase and cysteine cathepsin inhibition. Dent Mater 30:e133–e134.  https://doi.org/10.1016/jdental201408275 CrossRefGoogle Scholar
  46. 46.
    Osorio R, Yamauti M, Osorio E, Román JS, Toledano M (2011) Zinc-doped dentin adhesive for collagen protection at the hybrid layer. Eur J Oral Sci 119:401–410.  https://doi.org/10.1111/j1600-0722201100853x CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Dentistry, Dental Materials SectionUniversity of GranadaGranadaSpain
  2. 2.NanoMyP, Spin-Off EnterpriseUniversity of GranadaArmillaSpain

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