Clinical Oral Investigations

, Volume 22, Issue 7, pp 2663–2668 | Cite as

Induced surface proteins of Streptococcus epidermidis adhering to titanium implant substrata

  • R. BürgersEmail author
  • C. Morsczeck
  • O. Felthaus
  • M. Gosau
  • H.C. Beck
  • T. E. Reichert
Short Communication



Staphylococcus epidermidis, as a primary colonizer, is strongly associated with infections of (dental) implants (i.e., peri-implantitis), but little is known about the surface proteome of this bacterium. For the identification of bacterial adhesins, this study investigated the surface proteome of S. epidermidis adhering directly to titanium implant substrata.

Materials and methods

S. epidermidis strain ATTC 35984 was cultured either planktonically or on titanium implant specimens. The surface proteomes were isolated by mutanolysin digestion, and proteins were separated by 2D gel electrophoreses to reveal highly expressed proteins only. Protein spots were visualized by silver staining and proteins were identified by mass spectrometry.


Surface proteome analyses of S. epidermidis on titanium identified six expressed proteins. Three proteins were highly expressed on the titanium implants including accumulation-associated protein Q8CQD9. These specific proteins could be potential pathogenicity factors of bacteria in peri-implant biofilms.


For the first time, our study identified S. epidermidis surface proteins, which are expressed after adhesion to titanium implant materials.

Clinical relevance

Our study reveals possible candidates for a newly protein-based vaccine against peri-implantitis.


Peri-implantitis Staphylococcus epidermidis Surface proteome Titanium implants 



The work was supported by a grant of the Deutsche Forschungsgemeinschaft (DFG GO2297/1-1 & BU2697/2-1).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. No human participants were involved in the study.

Informed consent

For this type of study, formal consent is not required.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ProsthodonticsUniversity Medical Center GöttingenGöttingenGermany
  2. 2.Department of Oral and Maxillofacial SurgeryUniversity Hospital RegensburgRegensburgGermany
  3. 3.Department of Clinical Biochemistry and Pharmacology, Centre for Clinical ProteomicsOdense University HospitalOdenseDenmark

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