Sensitivity of caries pathogens to antimicrobial peptides related to caries risk
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Antimicrobial peptides (AMPs) represent important facets of the immune system controlling infectious diseases. However, pathogens show varying susceptibilities to AMPs. This study investigates the susceptibilities of strains of Streptococcus mutans (SM), Actinomyces naeslundii (AN), and Lactobacillus spp. (LB) towards AMPs and if there are correlations between the appearance of such high-risk strains and clinical caries status.
Material and methods
Plaque samples were collected from patients along with clinical examinations. Bacterial strains were identified via selective media, matrix-assisted laser desorption/ionization analysis-time of flight (MALDI-TOF), and arbitrary-primed-PCR (AP-PCR). Each strain was tested for susceptibility to LL-37, HBD-2, HNP-1, and HNP-3 or phosphate-buffered saline as negative control in a biofilm model on hydroxylapatite discs. Survival rates and resulting risk classification for each strain were determined. Correlations were calculated between the number of high-risk strains (all/S. mutans) appearing in patients and their clinical caries status.
Forty-seven patients were included with mean DMFT values of 11.4 ± 8.7. A total of 8 different SM, 30 LB, and 47 AN strains were detected. One-way ANOVA indicated that type/concentration of AMPs had major influence on reductions of Lactobacilli and Actinomyces. Seventeen strains of AN, 2 of SM, and 6 of LB had low susceptibilities to AMPs. The number of such strains in patients showed significant positive correlations to the DMFT values (all p = 0.001; r = 0.452; S. mutans p < 0.0001, r = 0.558).
The occurrence of low susceptible strains to AMPs seems to correlate with the individual caries status.
The results may lead to new ways to identify individuals with increased caries risk.
KeywordsAntimicrobial peptides Caries Biofilm Susceptibility
The authors would like to thank all participants who were included in this study. Further, we want to thank the team of the Department of Medical Microbiology of the Max-von-Pettenkofer-Institute for their help with the MALDI-TOF analysis.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
- 1.Jepsen S, Blanco J, Buchalla W, Carvalho JC, Dietrich T, Dörfer C, Eaton KA, Figuero E, Frencken JE, Graziani F, Higham SM, Kocher T, Maltz M, Ortiz-Vigon A, Schmoeckel J, Sculean A, Tenuta LM, van der Veen MH, Machiulskiene V (2017) Prevention and control of dental caries and periodontal diseases at individual and population level: consensus report of group 3 of joint EFP/ORCA workshop on the boundaries between caries and periodontal diseases. J Clin Periodontol 44 Suppl 18:S85–S93. https://doi.org/10.1111/jcpe.12687 CrossRefPubMedGoogle Scholar
- 2.Jordan AR, Micheelis W (2016) Fünfte Deutsche Mundgesundheitsstudie [DMS V]. Institut der Deutschen Zahnärzte [IDZ], KölnGoogle Scholar
- 4.Vilas Boas LC, de Lima LM, Migliolo L, Mendes GD, de Jesus MG, Franco OL, Silva PA (2017) Linear antimicrobial peptides with activity against herpes simplex virus 1 and Aichi virus. Biopolymers 108(2). https://doi.org/10.1002/bip.22871
- 5.Lima SMF, Freire MS, Gomes ALO, Cantuária APC, Dutra FRP, Magalhães BS, Sousa MGC, Migliolo L, Almeida JA, Franco OL, Rezende TMB (2017) Antimicrobial and immunomodulatory activity of host defense peptides, clavanins and LL-37, in vitro: an endodontic perspective. Peptides 95:16–24. https://doi.org/10.1016/j.peptides.2017.07.005 CrossRefPubMedGoogle Scholar
- 11.Harder J, Meyer-Hoffert U, Teran LM, Schwichtenberg L, Bartels J, Maune S, Schröder JM (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22(6):714–721. https://doi.org/10.1165/ajrcmb.22.6.4023 CrossRefPubMedGoogle Scholar
- 16.Lee SI, Kang SK, Jung HJ, Chun YH, Kwon YD, Kim EC (2015) Muramyl dipeptide activates human beta defensin 2 and pro-inflammatory mediators through toll-like receptors and NLRP3 inflammasomes in human dental pulp cells. Clin Oral Investig 19(6):1419–1428. https://doi.org/10.1007/s00784-014-1361-8 CrossRefPubMedGoogle Scholar
- 18.Dommisch H, Winter J, Götz W, Miesen J, Klein A, Hierse L, Deschner J, Jäger A, Eberhard J, Jepsen S (2015) Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing. Clin Oral Investig 19(2):209–220. https://doi.org/10.1007/s00784-014-1239-9 CrossRefPubMedGoogle Scholar
- 20.Ouhara K, Komatsuzawa H, Shiba H, Uchida Y, Kawai T, Sayama K, Hashimoto K, Taubman MA, Kurihara H, Sugai M (2006) Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells. Infect Immun 74(9):5211–5220. https://doi.org/10.1128/IAI.00056-06 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Nishimura E, Eto A, Kato M, Hashizume S, Imai S, Nisizawa T, Hanada N (2004) Oral streptococci exhibit diverse susceptibility to human β-dedensin-2: antimicrobial effects of hBD-2 on oral streptococci. Curr Microbiol 48:85–87Google Scholar
- 27.Ouhara K, Komazsuzawa H, Yamaa S, Shiba H, Fujiwara T, Ohara M, Sayama K, Hashimoto K, Kurihara H, Sugai M (2005) Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, β-defensins and LL-37, produced by human epithelial cells. J Antimicrob Chemother 55:888–896CrossRefPubMedGoogle Scholar
- 28.Phattarataratip E, Olson B, Broffitt B, Qian F, Brogden KA, Drake DR, Levy SM, Banas JA (2011) Streptococcus mutans strains recovered from caries-active or caries-free individuals differ in sensitivity to host anti-microbial peptides. Mol Oral Microbiol 26(3):187–199. https://doi.org/10.1111/j.2041-1014.2011.00607.x CrossRefPubMedPubMedCentralGoogle Scholar
- 29.Tao R, Jurevic RJ, Coulton KK, Tsutsui MT, Roberts MC, Kimball JR, Wells N, Berndt J, Dale BA (2005) Salivary antimicrobial peptide expression and dental caries experience in children. Antimicrob Agents Chemother 49(9):3883–3888. https://doi.org/10.1128/AAC.49.9.3883-3888.2005 CrossRefPubMedPubMedCentralGoogle Scholar
- 32.Wan AK, Seow WK, Walsh LJ, Bird PS (2002) Comparison of five selective media for the growth and enumeration of Streptococcus mutans. Aust Dent J 47(1):21–26. https://doi.org/10.1111/j.1834-7819.2002.tb00298.x CrossRefPubMedGoogle Scholar
- 37.Al Shukairy H, Alamoudi N, Farsi N, Al Mushayt A, Masoud I (2006) A comparative study of Streptococcus mutans and lactobacilli in mothers and children with severe early childhood caries (SECC) versus a caries free group of children and their corresponding mothers. Clin Pediatr Dent 31:80–85CrossRefGoogle Scholar
- 39.Gorr SU, Abdolhosseini M (2011) Antimicrobial peptides and periodontal disease. J Clin Periodontol 39:1028–1032Google Scholar