Clinical Oral Investigations

, Volume 22, Issue 5, pp 2069–2079 | Cite as

Therapeutic effect of andiroba oil (Carapa guianensis Aubl.) against oral mucositis: an experimental study in golden Syrian hamsters

  • Ana Márcia Viana Wanzeler
  • Sergio Melo Alves Júnior
  • Jessica Teixeira Gomes
  • Eduardo Henrique Herbster Gouveia
  • Higor Yuri Bezerra Henriques
  • Rosa Helena Chaves
  • Bruno Moreira Soares
  • Hugo Leonardo Crisóstomo Salgado
  • Alberdan Silva Santos
  • Fabrício Mesquita Tuji
Original Article



The aim of this study was to investigate the healing activity of andiroba (Carapa guianensis Aubl.) against oral mucositis (OM) induced by 5-fluorouracil in golden Syrian hamsters.

Materials and methods

A total of 122 animals were randomized and divided into six groups: andiroba oil 100%, andiroba oil 10%, andiroba oil 10% refined, no treatment group, all n = 28; and negative control (NC) and cyclophosphamide (CPA) groups, both n = 5. OM was induced by intraperitoneal administration of 60 mg/kg 5-FU on days 0, 5 and 10 followed by mechanical trauma on the oral mucosa on days 1 and 2. From day 1 to day 15, the animals of the andiroba group were treated three times a day. On days 4, 8, 12 and 15, the mucosa was photographed and removed for clinical and histopathological analysis. The bone marrow of the femur was removed and the micronucleus test was performed to evaluate the cytotoxicity and genotoxicity. The data were subjected to analysis of variance, followed by the Tukey and Bonferroni test.


Treatment with 100% andiroba oil reduced the degree of OM compared to that reported in the other groups (p < 0.05). Andiroba oil at both concentrations was not cytotoxic, but treatment with 100% andiroba oil showed a genotoxic potential (p < 0.001).


Frequent administration of andiroba oil accelerated the healing process in an experimental model of 5-fluorouracil-induced OM. However, the genotoxicity of andiroba in other cell systems and under other conditions are being tested.

Clinical relevance

The use of andiroba in topical form may be associated with reduced intensity of OM. Seek therapeutic alternatives to minimize the pain and suffering that these side effects cause cancer patients is an important scientific step.


Oral mucositis Cancer Oncology 5-fluorouracil Carapa guianensis Phytotherapy 



The authors thank the Experimental Research Group and the Laboratory of Histopathology of the University of the State of Pará (CESUPA) for the technical assistance provided by the Laboratory of Systematic Research in Biotechnology and Molecular Biodiversity of the Federal University of Pará (LabISisBio/UFPA) and by the Laboratory of Human Cytogenetics of the Federal University of Pará (LCH/UFPA).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies involving human participants performed by any of the authors. All procedures performed in studies with animals were in accordance with the ethical standards of the institutional and/or national research committee and all the care and use of animals were followed.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Ana Márcia Viana Wanzeler
    • 1
    • 2
  • Sergio Melo Alves Júnior
    • 1
  • Jessica Teixeira Gomes
    • 1
  • Eduardo Henrique Herbster Gouveia
    • 3
  • Higor Yuri Bezerra Henriques
    • 3
  • Rosa Helena Chaves
    • 3
  • Bruno Moreira Soares
    • 4
  • Hugo Leonardo Crisóstomo Salgado
    • 5
  • Alberdan Silva Santos
    • 5
  • Fabrício Mesquita Tuji
    • 1
  1. 1.Department of OdontologyFederal University of Pará, BelémBelémBrazil
  2. 2.BelémBrazil
  3. 3.Department of MedicineUniversity of the State of ParáBelémBrazil
  4. 4.Department of GeneticsHuman Cytogenetics Laboratory of the Federal University of ParáBelémBrazil
  5. 5.Department of ChemistrySystematic Research in Biotechnology and Molecular Diversity Laboratory of the Federal University of ParáBelémBrazil

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