Prognostic implication of NOTCH1 in early stage oral squamous cell cancer with occult metastases
The objective of this study was to explore the prognostic value of cancer stem cell markers, namely CD133, NANOG, and NOTCH1, in early stage oral squamous cell carcinoma (OSCC).
Materials and methods
One hundred forty-four patients with early stage (cT1T2N0) OSCC were identified from a pre-existing database of patients with oral cancer. We examined the impact of the immunohistochemical expression of CD133, NANOG, and NOTCH1 in OSCC. Overall survival (OS) curves were calculated using the Kaplan–Meier method. Predictors of outcome were identified using multivariate analysis.
We found that CD133, NANOG, and NOTCH1 were significantly associated with lymph node metastasis, and NOTCH1 was also significantly associated with depth of invasion and locoregional recurrence.
NOTCH1 was identified as an independent prognostic factor for OS.
NOTCH1 might prove to be a useful indicator for high-risk patients with occult metastases from early stage OSCC.
KeywordsCD133 NANOG NOTCH1 Oral squamous cell carcinoma Early stage
This work was supported by grants from the Doctoral Program Foundation of First Affiliated Hospital of Harbin Medical University in China (no. 2016B015).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the First Affiliated Hospitals of Harbin Medical University Institutional Research Board and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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