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Visualization of hydrogen polysulfides in living cells and in vivo via a near-infrared fluorescent probe

  • Xiaoqing WangEmail author
  • Qian Sun
  • Liming Zhao
  • Shuwen Gong
  • Li XuEmail author
Original Paper

Abstract

Hydrogen polysulfides (H2Sn, n > 1), as the oxidized forms of H2S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H2Sn in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1, with “turn-on” response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H2Sn. The probe was successfully applied to the visualizing of endogenous H2Sn in living cells. Moreover, it can be used for near-infrared in vivo imaging of H2Sn in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H2Sn in living systems.

Keywords

Fluorescent sensing and imaging Hydrogen polysulfides BODIPY Near-infrared 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21971115, 21501085) and Key University Science Research Project of Jiangsu Province (17KJA150004).

Supplementary material

775_2019_1718_MOESM1_ESM.pdf (976 kb)
Supplementary material 1 (PDF 976 kb)

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Copyright information

© Society for Biological Inorganic Chemistry (SBIC) 2019

Authors and Affiliations

  1. 1.College of ScienceNanjing Forestry UniversityNanjingChina
  2. 2.School of Chemistry and Chemical EngineeringLiaocheng UniversityLiaochengChina
  3. 3.Institute of Material Physics and ChemistryNanjing Forestry UniversityNanjingChina

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