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Effect of Crotalus basiliscus snake venom on the redox reaction of myoglobin

  • Ticyano P. de Souza
  • Dieric S. Abreu
  • Marta S. P. Carepo
  • Maria A. S. Silva
  • Dávila Zampieri
  • Marcos N. Eberlin
  • Tércio F. Paulo
  • Eduardo H. S. Sousa
  • Elisane Longhinotti
  • Izaura C. N. DiógenesEmail author
Original Paper
  • 172 Downloads

Abstract

In this work, we have studied the effect of Crotalus basiliscus snake venom on the redox reaction of myoglobin (Mb), and by means of electrochemical techniques, we have shown that this reaction is undoubtedly affected following the interaction with the venom. Surface plasmon resonance, electrophoresis, UV–Vis, and circular dichroism showed that the interaction involves the attachment of some constituent of the venom to the protein, although not affecting its first and secondary structures. Mass spectra support this suggestion by showing the appearance of signals assigned to the Mb dimer and to a new species resulting from the interaction between Mb and the venom proteins. In addition, the mass spectra suggest the aromatic amino acids of myoglobin, mainly tryptophan and phenylalanine, are more exposed to the solvent medium upon the exposure to the venom solution. The results altogether indicate that the harmful effects of the venom of Crotalus basiliscus snake are likely connected to the blocking of the redox site of Mb.

Keywords

Electrochemistry Modified electrode Myoglobin Snake venom Surface plasmon resonance 

Notes

Acknowledgements

I.C.N. Diógenes (# 307078/2017-5), T. F. Paulo (#428741/2016-9), E. Longhinotti (#306305/2015-1), and E.H.S. Sousa (# 312030/2015-0, #403866/2016-2) are thankful to CNPq and FUNCAP (PRONEX/2015 PR2-0101-00030.01.00/15 SPU No.: 3265612/2015) for the Grants and financial support. The authors are all grateful to P.V. Bernhardt (Queensland University) for his valuable comments and suggestions.

Supplementary material

775_2019_1636_MOESM1_ESM.pdf (527 kb)
Supplementary material 1 (PDF 527 kb)

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Copyright information

© Society for Biological Inorganic Chemistry (SBIC) 2019

Authors and Affiliations

  1. 1.Departamento de Química Orgânica e InorgânicaUniversidade Federal do CearáFortalezaBrazil
  2. 2.Departamento de FísicaUniversidade Federal do CearáFortalezaBrazil
  3. 3.LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparicaPortugal
  4. 4.ThoMSon Mass Spectrometry Laboratory, Institute of ChemistryUniversity of CampinasCampinasBrazil
  5. 5.Departamento de Química Analítica e Físico-QuímicaUniversidade Federal do CearáFortalezaBrazil

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