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A hydrogen peroxide biosensor based on the direct electrochemistry of hemoglobin modified with quantum dots

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Abstract

Direct electron transfer of hemoglobin modified with quantum dots (QDs) (CdS) has been performed at a normal graphite electrode. The response current is linearly dependent on the scan rate, indicating the direct electrochemistry of hemoglobin in that case is a surface-controlled electrode process. UV–vis spectra suggest that the conformation of hemoglobin modified with CdS is little different from that of hemoglobin alone, and the conformation changes reversibly in the pH range 3.0–10.0. The hemoglobin in a QD film can retain its bioactivity and the modified electrode can work as a hydrogen peroxide biosensor because of its peroxidase-like activity. This biosensor shows an excellent response to the reduction of H2O2 without the aid of an electron mediator. The catalytic current shows a linear dependence on the concentration of H2O2 in the range 5 × 10−7–3 × 10−4 M with a detection limit of 6 × 10−8 M. The response shows Michaelis–Menten behavior at higher H2O2 concentrations and the apparent Michaelis–Menten constant is estimated to be 112 μM.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (nos. 30370397, 60571042 and 20575046), Specialized Research Fund for the Doctoral Program of Higher Education (20050486026) and the Science Fund for Creative Research Groups (no. 20621502), NSFC.

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yanxia Xu, Jiangong Liang, Chengguo Hu, Fang Wang, Shengshui Hu & Zhike He

  2. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Yanxia Xu & Shengshui Hu

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  1. Yanxia Xu
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  2. Jiangong Liang
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  3. Chengguo Hu
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  4. Fang Wang
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Correspondence to Shengshui Hu or Zhike He.

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Xu, Y., Liang, J., Hu, C. et al. A hydrogen peroxide biosensor based on the direct electrochemistry of hemoglobin modified with quantum dots. J Biol Inorg Chem 12, 421–427 (2007). https://doi.org/10.1007/s00775-006-0198-2

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  • DOI: https://doi.org/10.1007/s00775-006-0198-2

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