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Maternal 25-hydroxycholecalciferol during lactation improves intestinal calcium absorption and bone properties in sow-suckling piglet pairs

  • Lianhua Zhang
  • Jiangxu Hu
  • Miao Li
  • Qinghui Shang
  • Sujie Liu
  • Xiangshu PiaoEmail author
Original Article

Abstract

Lower maternal vitamin D status during lactation is a common health problem. The objectives of this study were to investigate the effects of maternal 25-hydroxycholecalciferol (25-OH-D3) supplementation during lactation on maternal and neonatal bone health in a sow model. 32 Large White × Landrace sows were assigned randomly to one of two diets supplemented with 2000 IU/kg vitamin D3 (ND) or 50 μg/kg 25-OH-D3 (25-D). The experiment began on day 107 of gestation and continued until weaning on day 21 of lactation. Maternal 25-OH-D3 supplementation significantly decreased milk n-6:n-3 PUFA ratio, which supported bone formation of piglets. Supplementation with 25-OH-D3 altered bone turnover rate of sows and piglets, as evidenced by higher bone-specific alkaline phosphatase (BALP) concentration in serum. 25-D sows had significantly higher bone density and mechanical properties of tibias and femurs than ND sows. Calcium (Ca) absorption rate was higher in 25-D sows than ND sows, which was caused partially by the increased mRNA expressions of renal 1α-hydroxylase (CYP27B1) and duodenal vitamin D receptor (VDR), transient receptor potential vanilloid 6 (TRPV6), and calcium-binding protein D9k (CaBP-D9k). Maternal 25-OH-D3 supplementation increased tibial and femoral Ca content by up-regulating Ca-related gene expression in kidney (CYP27B1), ileum (VDR and claudin-2), and colon (VDR and CaBP-D9k), thus, activating 1,25-dihydroxyvitamin D3 [1,25-(OH)2-D3]-dependent Ca transport in piglets. In conclusion, improved milk fatty acids and higher mRNA expressions of calcitropic genes triggered by maternal 25-OH-D3 supplementation would be the potential mechanism underlying the positive effects of 25-OH-D3 on maternal and neonatal bone health.

Keywords

Maternal 25-hydroxycholecalciferol Milk fatty acids Calcium absorption Bone Piglets 

Notes

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (No. 31772612). We gratefully acknowledge Haineng Bioengineering Co., Ltd. (Rizhao, China) who provides us with experimental product and scientific funding used in this study.

Author contributions

The authors’ contributions are as follows: XP and LZ designed research; LZ carried out the experiment design and wrote the draft of manuscript; LZ, JH, and ML were responsible for the data analysis; LZ, QS, and SL reviewed and revised the manuscript; XP had primary responsibility for final content. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

All authors have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

774_2019_1020_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 21 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Animal Nutrition, College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina

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